Evaluation of the toxicity of gold nanoparticles produced by green nanotechnology in Zebrafish (Danio rerio)
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2018
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WORKSHOP ON ENVIRONMENTAL NANOTECHNOLOGY, 3rd
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Gold nanoparticles (AuNPs) of different sizes and shapes have been extensively studied by researchers and
laboratories around the world. Several studies have demonstrated the applicability of gold nanoparticles in the
treatment and diagnosis of cancer, in the treatment of chronic inflammation, infections, degenerative diseases
and autoimmune diseases [1]. The synthesis of AuNPs generally involves reducing agents which present
problems related to toxicity. In order to address this issue, metabolites present in various plant extracts have
been exploited for the preparation of different nanoparticles. The methods that use phytochemicals to reduce
metal ions provide a green approach to nanotechnology, known as green nanotechnology [2]. Researchers have
shown that some phytochemicals, such as mangiferin (MGF) and epigallocatechin-gallate (EGCG), in addition to
reducing and stabilizing the gold nanoparticles, are able to functionalize them. These molecules have chemical
groups that allow binding to overexpressed receptors on some types of tumor cells [3]. The objective of this
study was to evaluate the level of toxicity of the gold nanoparticles, reduced and stabilized with
epigallocatechin-gallate (EGCG-AuNPs) in Zebrafish embryos (Danio rerio), as an indication of a possible
environmental effect. To assess the developmental impact of embryos, organisms were exposed to different
dilutions of the EGCG-AuNPs suspension for a 96-hour period according to OECD Protocol 236 (Fish Embryo
Acute Toxicity Test-FET). Zebrafish is an established vertebrate model for the study of development, disease
and is being increasingly used for both pre-clinical studies and toxicological applications due to a range of
favorable traits [4]. EGCG-AuNPs demonstrated toxicity, with organ lethality being less than 33% at all
concentrations used. The work provided a contribution on the toxicity of AuNPs synthesized and stabilized with
the epigallocatechin-gallate reducing agent and using Zebrafish embryos as an animal.
Como referenciar
CAVALCANTE, A.K.; BATISTA, J.G.S.; MAZIERO, J.S.; DAMASCENO, K.C.; ORMENIO, M.B.; CAVALCANTE, B.K.; ROGERO, S.O.; ROGERO, J.R.; LUGAO, A.B. Evaluation of the toxicity of gold nanoparticles produced by green nanotechnology in Zebrafish (Danio rerio). In: WORKSHOP ON ENVIRONMENTAL NANOTECHNOLOGY, 3rd, October 5-8, 2018, Sorocaba, SP. Abstract... p. 28-28. Disponível em: http://repositorio.ipen.br/handle/123456789/30122. Acesso em: 03 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.