Red LED irradiation impacts the cytotoxic response of murine breast cancer cells to ionizing radiation
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INTRODUCTION
Breast cancer is a disease of worldwide importance since it is considered
the 5th leading cause of cancer deaths. Triple-negative breast cancer
(TNBC) is a molecular subtype that presents resistance to conventional
radiotherapy, demanding high doses of ionizing radiation (IR) for a
prolonged period of treatment. On the other hand, low-level light irradiation
(LLLI) has been studied to sensitize cells before IR exposure.
However, the literature is poor regarding the association of both techniques
in TNBC cells.
OBJECTIVES
Thus, we aimed to assess the effect of LLLI before IR exposure on two
TNBC cell lineages.
MATERIALS AND METHODS
MDA-MB-231 (human TNBC) and 4T1 (murine TNBC) were cultivated,
seeded at a density of 2.5 x 10 5 cells/cm², and maintained in an
incubator (37ºC, 5% of CO2) overnight. LLLI was performed with a
red LED (λ= 660 ± 11 nm, 38.2 mW/cm²) delivering energies of 1.2 J
and 6.0 J. One-h after LLLI, the cells were submitted to both 2.5 and
5.0 Gy doses from a 60 CO source. After 24-h, mitochondrial activity
(MA) was quantified by MTT assay with n= 9/group.
DISCUSSION AND RESULTS
Our data showed that 4T1 cells exposed to LLLI at 1.2 J exhibited higher
MA than cells exposed to IR2.5. In contrast, cells exposed to 6 J of LLLI
showed lower MA than IR5. Concerning MDA-MB231 cells, no statistically
significant differences were noticed among groups regardless of IR
and LLLI doses.
CONCLUSION
These findings indicate that LLLI before IR could sensitize only murine
breast cancer. Besides, an appropriate combination of IR and LLLI doses
seems to play a role to kill TNBC cells.
Como referenciar
PINTO, MAYARA S.; SILVA, CAMILA R.; SALVEGO, CAMILA de A.; SIMOES, MARTHA R. Red LED irradiation impacts the cytotoxic response of murine breast cancer cells to ionizing radiation. Biophysical Reviews, v. 13, n. 6, p. 1385-1385, 2021. DOI: 10.1007/s12551-021-00845-2. Disponível em: http://repositorio.ipen.br/handle/123456789/32888. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.