Radiolabeling and biological evaluation of the GX1 and RGD-GX1 peptide sequence for angiogenesis teargeting
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Nuclear Medicine and Biology
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Introduction: Aiming to develop a novel 99mTc-labeled imaging agent, for angiogenesis and tumor receptors, two
peptides obtained from phage display library, namely GX1 and the heterodimer RGD-GX1, were synthesized in a
cyclic conformation. They were radiolabeled with 99mTc, employing the HYNIC chelator, for radiochemical evaluation and biological properties.
Methods: Radiolabeling, radiochemical control, plasma protein binding, and partition coefficient were assessed
for both radioconjugates. Biodistribution in healthy Balb/c mice was carried out, in order to evaluate the biological behaviour of the radiocomplexes.
Results: The conjugates displayed a rather similar pharmacokinetic profile. They were prepared with high radiochemical purity (N96%), and both were hydrophilic (log P of −2.25 and −2.51 respectively). Preferential renal
excretion was observed. Kidney uptake (42.31 ± 5.35 %ID/g) for 99mTc-HYNIC-E-[c(RGDfk)-c(GX1)], 1 h postinjection was about three times higher than the uptake of 99mTc-HYNIC-PEG4-c(GX1) (11.92 ± 4.77%ID/g).
Total blood, bone and muscle values revealed a slightly slower clearance for the RGD-GX1 radiocomplex.
Conclusion: The high radiochemical purity achieved, and the similar in vivo profile observed for both
radioconjugates, make them potential candidates for radiopharmaceuticals for tumor imaging. Further investigations of binding affinity, and uptake of GX1 and RGD-GX1 peptides in tumor models, are warranted.
Como referenciar
OLIVEIRA, E.A.; FAINTUCH, B.L. Radiolabeling and biological evaluation of the GX1 and RGD-GX1 peptide sequence for angiogenesis teargeting. Nuclear Medicine and Biology, v. 42, p. 123-130, 2015. DOI: 10.1016/j.nucmedbio.2014.09.004. Disponível em: http://repositorio.ipen.br/handle/123456789/23261. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.