Endostatin, an antiangiogenic protein, is expressed in the unilateral ureteral obstruction mice model
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Journal of Nephrology
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Background: Extracellular matrix accumulation, epithelial-to-mesenchymal transition, tubular atrophy and loss
of peritubular capillary network are hallmarks of tubulointerstitial injury in progressive renal diseases. In this
study, we analyzed endostatin expression in kidneys
subjected to unilateral ureteral obstruction (UUO).
Methods: Collagen XVIII mRNA expression was evaluated by real-time polymerase chain reaction (PCR). Endostatin and CD31 protein levels were analyzed by Western
blot and immunohistochemistry. In vitro quantification of
collagen XVIII and fibrosis-related genes in HK2 cells
was performed by real-time PCR.
Results: UUO significantly increased collagen XVIII mRNA expression and released a 30-kDa endostatin fragment. Immunohistochemistry revealed endostatin expression increased in injured tissue, mainly on tubular
cells. Of interest, expression of CD31 was significantly
reduced by UUO. Endostatin administration in vitro did
not modify the expression of genes related to fibrosis development. However, in vitro TGF-b1 administration induced expression of collagen XVIII/endostatin mRNA in
human tubular cells.
Conclusion: Endostatin is expressed during the progression of renal fibrosis in vitro and in vivo, suggesting a
role for endostatin in development of tubulointerstitial injury.
Como referenciar
MACIEL, THIAGO T.; COUTINHO, ENIA L.; SOARES, DEBORA; ACHAR, EDUARDO; SCHOR, NESTOR; BELLINI, MARIA H. Endostatin, an antiangiogenic protein, is expressed in the unilateral ureteral obstruction mice model. Journal of Nephrology, v. 21, p. 753-760, 2008. Disponível em: http://repositorio.ipen.br/handle/123456789/26632. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.