Sandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70)

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dc.contributor.authorNEGAHDARY, MASOUDpt_BR
dc.contributor.authorHIRATA, MARIO H.pt_BR
dc.contributor.authorSAKATA, SOLANGE K.pt_BR
dc.contributor.authorCICONELLI, ROZANA M.pt_BR
dc.contributor.authorBASTOS, GISELE M.pt_BR
dc.contributor.authorBORGES, JESSICA B.pt_BR
dc.contributor.authorTHUROW, HELENA S.pt_BR
dc.contributor.authorSILVEIRA JUNIOR, ALCEU T.pt_BR
dc.contributor.authorSAMPAIO, MARCELO F.pt_BR
dc.contributor.authorGUIMARAES, LARISSA B.pt_BR
dc.contributor.authorMAEDA, BRUNO S.pt_BR
dc.contributor.authorANGNES, LUCIOpt_BR
dc.coverageInternacional
dc.date.accessioned2023-04-14T15:49:56Z
dc.date.available2023-04-14T15:49:56Z
dc.date.issued2023pt_BR
dc.description.abstractIn this research, by using aptamer-conjugated gold nanoparticles (aptamer-AuNPs) and a modified glassy carbon electrode (GCE) with reduced graphene oxide (rGO) and Acropora-like gold (ALG) nanostructure, a sandwich-like system provided for sensitive detection of heat shock protein 70 kDa (HSP70), which applied as a functional biomarker in diagnosis/prognosis of COVID-19. Initially, the surface of the GCE was improved with rGO and ALG nanostructures, respectively. Then, an aptamer sequence as the first part of the bioreceptor was covalently bound on the surface of the GCE/rGO/ALG nanostructures. After adding the analyte, the second part of the bioreceptor (aptamer-AuNPs) was immobilized on the electrode surface to improve the diagnostic performance. The designed aptasensor detected HSP70 in a wide linear range, from 5 pg mL−1 to 75 ng mL−1, with a limit of detection (LOD) of ∼2 pg mL−1. The aptasensor was stable for 3 weeks and applicable in detecting 40 real plasma samples of COVID-19 patients. The diagnostic sensitivity and specificity were 90% and 85%, respectively, compared with the reverse transcription-polymerase chain reaction (RT-PCR) method.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDFAPESP: 19/27021-4; 17/13137-5; 14/50867-3pt_BR
dc.description.sponsorshipIDCNPq: 311847/2018-8; 465389/2014-7pt_BR
dc.format.extent1-10pt_BR
dc.identifier.citationNEGAHDARY, MASOUD; HIRATA, MARIO H.; SAKATA, SOLANGE K.; CICONELLI, ROZANA M.; BASTOS, GISELE M.; BORGES, JESSICA B.; THUROW, HELENA S.; SILVEIRA JUNIOR, ALCEU T.; SAMPAIO, MARCELO F.; GUIMARAES, LARISSA B.; MAEDA, BRUNO S.; ANGNES, LUCIO. Sandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70): application in diagnosis/prognosis of coronavirus disease 2019 (COVID-19). <b>Analytica Chimica Acta</b>, v. 1242, p. 1-10, 2023. DOI: <a href="https://dx.doi.org/10.1016/j.aca.2022.340716">10.1016/j.aca.2022.340716</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/33975.
dc.identifier.doi10.1016/j.aca.2022.340716pt_BR
dc.identifier.issn0003-2670
dc.identifier.orcid0000-0001-6072-5853pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6072-5853
dc.identifier.percentilfi90.1
dc.identifier.percentilfiCiteScore87.50
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/33975
dc.identifier.vol1242pt_BR
dc.relation.ispartofAnalytica Chimica Acta
dc.rightsopenAccesspt_BR
dc.subjectgold
dc.subjectnanomaterials
dc.subjectcoronaviruses
dc.subjectelectrochemistry
dc.subjectelectrodes
dc.subjectgraphene
dc.titleSandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70)pt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorSOLANGE KAZUMI SAKATA
ipen.codigoautor7038
ipen.contributor.ipenauthorSOLANGE KAZUMI SAKATA
ipen.date.recebimento23-04
ipen.identifier.fi5.7
ipen.identifier.fiCiteScore10.4
ipen.identifier.ipendoc29599
ipen.identifier.iwosWoSpt_BR
ipen.range.fi4.500 - 5.999
ipen.range.percentilfi75.00 - 100.00
ipen.subtituloapplication in diagnosis/prognosis of coronavirus disease 2019 (COVID-19)pt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublicationfb62f288-104d-4352-aed0-a18e0da21a1b
relation.isAuthorOfPublication.latestForDiscoveryfb62f288-104d-4352-aed0-a18e0da21a1b
sigepi.autor.atividadeSAKATA, SOLANGE K.:7038:220:Npt_BR
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