Increased adiposity by feeding growing rats a high-fat diet results in iron decompartmentalisation
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British Journal of Nutrition
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The present study reports the effects of a high-fat (HF) diet of over 8 weeks on the Fe status of growing rats. Tissue Fe levels were analysed by
atomic absorption spectrophotometry, and whole-body adiposity was measured by dual-energy X-ray absorptiometry. Histopathology and
morphometry of adipose tissue were performed. Liver homogenates were used for measuring ferroportin-1 protein levels by immunoblotting,
and transcript levels were used for Fe genes measured by real-time PCR. Tissue Fe pools were fit to a compartmental biokinetic model in which
Fe was assessed using fourteen compartments and twenty-seven transfer constants (kj,i from tissue ‘i’ to tissue ‘j’) adapted from the International
Commission on Radiological Protection (ICRP) 69. Ten kj,i were calculated from the experimental data using non-linear regression, and seventeen
were estimated by allometry according to the formula ki;j ¼ a ×Mb. Validation of the model was carried out by comparing predicted and
analysed Fe pool sizes in erythrocytes, the liver and the spleen. Body adiposity was negatively associated with serum Fe levels and positively
associated with liver Fe stores. An inferred increase in Fe transfer from bone marrow to the liver paralleled higher hepatic Fe concentrations and
ferritin heavy-chain mRNA levels in the HF diet-fed animals, suggesting that liver Fe accumulation occurred at least in part due to a favoured liver
erythrocyte uptake. If this feeding condition was to be prolonged, impaired Fe decompartmentalisation may occur, ultimately resulting in
dysmetabolic Fe overload.
Como referenciar
LOBO, ALEXANDRE R.; GAIEVSKI, EDUARDO H.S.; MESQUITA, CARLOS H. de; DE CARLI, EDUARDO; TEIXEIRA, PRYSCILA D.S.; PEREIRA, ROSA M.R.; BORELLI, PRIMAVERA; SÁ, LILIAN R.M. de; COLLI, CELIA. Increased adiposity by feeding growing rats a high-fat diet results in iron decompartmentalisation. British Journal of Nutrition, v. 123, n. 10, p. 1094-1108, 2020. DOI: 10.1017/S0007114519002320. Disponível em: http://repositorio.ipen.br/handle/123456789/31329. Acesso em: 24 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.