Experimental design of size variation in albumin nanoparticles synthesized by electron beam
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ARYEL HEITOR FERREIRA
CASSIA PRISCILA CUNHA DA CRUZ
LUCAS FREITAS DE FREITAS
ADEMAR BENEVOLO LUGAO
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SSRN Electronic Journal
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Protein-based nanoparticles are of great interest for theragnostic applications such as cancer
treatment and nuclear medicine. Beyond that, plasma proteins are particularly attractive as they
can circumvent the rapid clearance of synthetic particles. Human serum albumin (HSA) is already
used for diagnosis purposes, such as lymphoscintigraphy and sentinel lymph node detection,
and in cancer treatment to deliver therapeutic agents in which the nanoparticulated form is
employed. Among the techniques adopted for protein nanoparticle synthesis, ionizing radiation
can provide good size control and preserve protein three-dimensional structure. Depending on
the reaction precursor, pH, protein concentration, presence of stabilizer, and irradiation dose, it is possible to tailor nanoparticles size and shape. Therefore, this work aimed to find a
correlation between albumin nanoparticle size and reaction parameters such as protein
concentrations, buffer solution, and e-beam irradiation dose. Different syntheses were
performed with varying BSA concentrations, two different buffers, and dose radiation. The
nanoparticles were then evaluated by Dynamic Light Scattering, and the data regarding sizes
were statistically analyzed by SAS studio, SAS JMP, and WEKA software to obtain any correlation
among the synthesis variables. It was mainly possible to observe that smaller protein
concentrations always lead to smaller particles and that using Tris-HCl buffer as media provided
a more proportional growth of particles according to concentration and dose irradiation
increases. Thus, these observations indicate that Tris buffer was more appropriate for BSA
nanoparticle synthesis as the increase of albumin concentration and radiation dose led to more
homogeneous size control.
Como referenciar
FERREIRA, ARYEL H.; LIMA, CAROLINE S.A.; DA CRUZ, CASSIA P.; FREITAS, LUCAS F.; FURLAN, GUSTAVO N.; DE LIMA, ROBSON C.; SARRIES, GABRIEL A.; LUGAO, ADEMAR B. Experimental design of size variation in albumin nanoparticles synthesized by electron beam. SSRN Electronic Journal, p. 1-26, 2023. DOI: 10.2139/ssrn.4631561. Disponível em: https://repositorio.ipen.br/handle/123456789/50066. Acesso em: 30 Jun 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.