Hepatic bioavailability of Total Mercury (THg) in Cichla sp from Tapajós River Region, Brazilian Amazon
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2014
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ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th
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Resumo
Most of the Hg occurring in the Amazon is of natural origin. However, the intense
gold mining activity in the region has increased the levels of this toxic metal,
especially in the middle section of the Tapajós River. Specimens of the Cichla
genus have great economic and ecological significance, as they are the most
consumed fish in the Brazilian Amazon region and are at the top of the Amazon
food chain. In addition, they are a good indicator of Hg bioaccumulation because
the concentrations of Hg in their tissues tend to reflect the accumulation process of
successive exposure. A total of 26 adult specimens of Cichla sp were obtained by
fisheries in the Tapajós River region’s contaminated gold mining area. In the
laboratory, morphometric data of each fish was taken, for instance total length, total
weight and sex. The liver tissue was removed from each specimen, washed with
distilled water, packed in polyethylene identified bags and kept at - 20ºC for
subsequent total mercury (THg) analysis. Assays were carry out in the total liver
and in the cytosol hepatic fraction obtained by ultracentrifugation of the tissue in
buffer Tris-HCl 10 mM, pH 7.4. The analyses were performed by flow injection
Cold Vapor Atomic Absorption Spectrometry (FIA-CV AAS) with detection (DL)
and quantification limits (QL) of 0.527 and 2.585 μg Kg-1, respectively. Individuals
of C. sp had active gonadal maturation, including spawning and post-spawning
individuals. The average length and total weight was 395 ± 51 mm and 840 ± 418 g,
respectively. High levels of THg were obtained in the total liver ranging from 1.7
mg Kg-1 to 3.8 mg Kg-1. However, only 0.2% of the THg were available to the
cytosol hepatic fraction. A significant and positive correlation (rs = 0.65) was found
between the concentration of the THg in the total tissue and in the hepatic cytosol
fraction. These data are a preliminary study with respect to the bioavailability of
total mercury (THg) in different compartments of the hepatic tissue of C. sp in order
to understand some aspects of the detoxification mechanism of this toxic metal in
bioindicator species from the Amazon region. In fact, it was possible to observe
differences in the transference of this important toxic metal into the cell and its linkage in the compartments.
Como referenciar
AZEVEDO, J.D.; SARKIS, J.E.; HORTELLANI, M.A. Hepatic bioavailability of Total Mercury (THg) in Cichla sp from Tapajós River Region, Brazilian Amazon. In: ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th, May 11-15, 2014, Basel, Switzerland. Abstract... p. 360-361. Disponível em: http://repositorio.ipen.br/handle/123456789/27656. Acesso em: 26 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.