State of the art and current advances on protein cross-linking by irradiation: protein based nanocarriers and bioactive nanoparticles
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2017
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INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY
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The highlighted role of protein and peptide based delivery systems relies upon the possibility
to develop biocompatible drug carriers featuring site specific delivery, biological affinity
among unique advantages. Recently, a technique for protein nanostructuring by the use
of radiation has been recently reported by our group. Advantages of the use of radiation
over conventional methods are related to the possibility to achieve protein cross-linking
and sterilization in a single step, as well as the capacity to allow the design of nanocarriers
without the need of monomers or toxic cross-linkers. This work reports the use of high
energy irradiation towards the design of size-controlled protein-based nanocarriers and
bioactive nanoparticles, using bovine serum albumin (BSA) and papain as model protein and
protease, respectively, including the state of the art and current advances of the technology.
The technique implies on protein desolvation/solvation techniques followed by cross-linking
by EB radiation or
-irradiation alone, although nanoparticles were also achieved in absence
of the cosolvents. Size-controlled BSA nanocarriers were manufactured up to 80 nm and
papain bioactive nanoparticles up to 12 nm, as determined by dynamic light scattering.
Nanocarrier morphology was evaluated by and negative staining transmission electron
microscopy. Protein cross-linking and changes in aromatic the amino acids were evaluated
by fluorescence measurements. Biocompatibility experimentswere also performed by means
of cytotoxicity and cytokines production. The potential of the systems for the delivery of
radiopharmaceuticals or chemotherapeutic agents were also assayed, using technetium or
Paclitaxel respectively. In conclusion, the technique allowed the production of biocompatible
and bioactive protein nanoparticles suitable for the administration of radiopharmaceuticals
and chemotherapeutic agents.
Como referenciar
VARCA, G.H.C.; MOHAMED, L.B.; FAZOLIN, G.N.; BATISTA, J.G.D.S.; QUEIROZ, R.G.; LUGAO, A.B.; NAVARRO MARQUES, F.A.; FERREIRA, A.H. State of the art and current advances on protein cross-linking by irradiation: protein based nanocarriers and bioactive nanoparticles. In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY, April 24-28, 2017, Vienna, Austria. Abstract... Vienna: IAEA, 2017. p. 171-171. Disponível em: http://repositorio.ipen.br/handle/123456789/27769. Acesso em: 19 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.