ANGELICA BUENO BARBEZAN

ANGELICA BUENO BARBEZAN

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Synthesis and characterization of Au-198 nanoparticles for radiotherapy
2024 - RIGO, M.; BARBEZAN, A.; TALACIMON, C.; TEODORO, L.; RODRIGUES, P.; ROSERO, W.; NOGUEIRA, T.; ROSTELATO, M.
Optimization of concentration and activity in the production of radioactive gold nanoparticles for cancer treatment
2024 - ALEXANDER, A.R.W.; ANGELICA, B.B.; FERNANDO, A.L.; ELISA, C.M.R.M.
Evaluations of the possible mutagenic and genotoxic effects of 2-ACBs
2024 - BARBEZAN, A.B.; BISPO, A.C.A.; MENDES, B.M.; VIEIRA, D.P.; CARVALHO, L.R.; MACHADO-SANTELLI, G.M.; VILLAVICENCIO, A.L.C.H.
In vivo distribution dynamics of gold nanoparticles
2024 - BARBEZAN, A.B.; ROSERO, W.A.W.; ALMEIDA, L.F.; RIGO, M.E.Z.; SILVA, F.F.A.; ROSTELATO, M.E.C.M.
Review of advances in coating and functionalization of gold nanoparticles
2024 - ROSERO, WILMMER A.A.; BARBEZAN, ANGELICA B.; SOUZA, CARLA D. de; ROSTELATO, MARIA E.C.M.
Nanoparticles, especially gold nanoparticles (Au NPs) have gained increasing interest in biomedical applications. Used for disease prevention, diagnosis and therapies, its significant advantages in therapeutic efficacy and safety have been the main target of interest. Its application in immune system prevention, stability in physiological environments and cell membranes, low toxicity and optimal bioperformances are critical to the success of engineered nanomaterials. Its unique optical properties are great attractors. Recently, several physical and chemical methods for coating these NPs have been widely used. Biomolecules such as DNA, RNA, peptides, antibodies, proteins, carbohydrates and biopolymers, among others, have been widely used in coatings of Au NPs for various biomedical applications, thus increasing their biocompatibility while maintaining their biological functions. This review mainly presents a general and representative view of the different types of coatings and Au NP functionalization using various biomolecules, strategies and functionalization mechanisms.
Radioactive gold nanoparticles coated with BSA
2024 - BARBEZAN, ANGELICA B.; ROSERO, WILMMER A.A.; VIEIRA, DANIEL P.; RIGO, MARIA E.Z.; SILVA, GIOVANA D. da; RODRIGUES, ALEX A.; ALMEIDA, LUIS F. de; SILVA, FABIO F.A. da; RIVERA, ANDY G.; SILVA, NATANAEL G. da; BERNARDES, EMERSON S.; ZEITUNI, CARLOS A.; ROSTELATO, MARIA E.C.M.
Background: Nanotechnology has revolutionized medicine, especially in oncological treatments. Gold nanoparticles (AuNPs) stand out as an innovative alternative due to their biocompatibility, potential for surface modification, and effectiveness in radiotherapeutic techniques. Given that prostate cancer ranks as one of the leading malignancies among men, there's a pressing need to investigate new therapeutic approaches. Methods: AuNPs coated with bovine serum albumin (BSA) were synthesized and their cytotoxicity was assessed against prostate tumor cell lines (LNCaP and PC-3), healthy prostate cells (RWPE-1), and endothelial control cells (HUVEC) using the MTS/PMS assay. For in vivo studies, BALB/C Nude mice were employed to gauge the therapeutic efficacy, biodistribution, and hematological implications post-treatment with BSA-coated AuNPs. Results: The BSA-coated AuNPs exhibited cytotoxic potential against PC-3 and LNCaP lines, while interactions with RWPE-1 and HUVEC remain subjects for further scrutiny. Within animal models, a diverse therapeutic response was observed, with certain instances indicating complete tumor regression. Biodistribution data emphasized the nanoparticles' affinity towards particular organs, and the majority of hematological indicators aligned with normative standards. Conclusions: BSA-coated AuNPs manifest substantial promise as therapeutic tools in treating prostate cancer. The present research not only accentuates the nanoparticles' efficacy but also stresses the imperative of optimization to ascertain both selectivity and safety. Such findings illuminate a promising trajectory for avant-garde therapeutic modalities, holding substantial implications for public health advancements.
Gold radioactive nanoparticles for brachytherapy
2023 - ROSERO, WILMMER A.A.; BARBEZAN, ANGELICA B.; ZEITUNI, CARLOS A.; ROSTELATO, MARIA E.C.M.
The development of new materials emerges as an alternative to the treatment of cancer, Nanobrachytherapy is born from the union of nanotechnology and brachytherapy being a modality of radiotherapy in which the nanosources are placed close to or in contact with the region to be treated. In nanoparticle synthesis, a key role is played by coatings, certain sizes of uncoated nanoparticles tend to accumulate in tumors due to vessel irregularities. When coated with gum arabic, the size of the nanoparticles is controlled, allowing the particles to properly penetrate the vasculature. The choice of radionuclide depends on the radiobiology of the cancer and the dose deposited in the tissue, which takes into account the type of decay (beta particles penetrate less into the tissue than X-rays and gamma rays), energy and half-life time (which influence the deposited dose per duration of radioisotope). In this work, 198Au will be used, which presents Beta as the main type of emission with an energy of 314.55 KeV, gamma 411.8 KeV, and a half-life of 2.7 days. The synthesis of radioactive nanoparticles is carried out in a closed chemical reactor, developed during the tests. The chemical reactor gold nanoparticles and precursor chloroauric acid are obtained. The morphology of nanoparticles and their size is guaranteed cold by TEM and radioactive by DLS. With an average diameter (TEM) of 5 nm, gum arabic nanoparticles have a curious property, when solution they form stable agglomerates of 45 nm (DLS), optimal size for in vitro and in vivo tests. They were tested with cancer cells of prostate and mice. In one of the evaluated LNCaP strains, 11% cytotoxicity was observed at the lowest concentration (0.9 μCi/well). At the concentration of 1.8 μCi/well, there was cell proliferation and at the highest concentration (2.7 μCi/well) it showed 32% cytotoxicity. After statistical analysis, the results revealed that although none of the animals showed regression of tumor mass. Tumor growth was slower at treated animals when compared to the tumor growth of the control animals (zero dose). Signaling us then that there is a positive effect on therapeutic efficacy, but these data suggest that the activity used was relatively low to achieve the regression of this mass. In the next experiments we will increase the injected radioactive activity to confirm these results and evaluate its real therapeutic potential.
Development of radioactive nanoparticles functionalized with gum arabic to be used in nanobrachytherapy
2023 - ROSERO, WILMMER A.A.; BARBEZAN, ANGELICA B.; RIGO, MARIA E.Z.; RODRIGUES, PRISCILA S.; TEODORO, LARA E.H.; TALACIMON, CRISTHIAN F.; ZEITUNI, CARLOS A.; ROSTELATO, MARIA E.C.M.
The development of new materials emerges as an alternative to the treatment of cancer, from the combination of nanotechnology and brachytherapy a new area of research was born, Nanobrachytherapy, which through the properties of nanometric materials can achieve better results in the fight against cancer. The objective of this work is classified as radiotherapy, which consists of the use of ionizing radiation to destroy or inhibit the growth of abnormal cells that form a tumor. [1] The ability to integrate NPsAu into biological systems is due to the nanometric dimensions of NPsAu probes which facilitate their incorporation into biological systems, as well as their bioconjugation and non-cytotoxic potential. [2] Taking into account the previous objective, gold was selected as the base element for obtaining nanometric systems, which due to its chemical richness and especially due to the intrinsic properties of one of its radioisotopes, which would allow us in theory to meet the stated objective. . Initially, it was based on the knowledge of the literature, and non-radioactive nanometric systems were obtained, and after a series of stability, characterization and application tests, the radioactive nanometric systems were obtained. Working with radioactive systems posed a great challenge, and up to now it presents us with situations to solve, but we have managed to create a methodology for obtaining, characterizing and applying radioactive gold nanoparticles, and also obtaining positive results from their application.
Synthesis, activation and application testing of gold nanoparticles for nanobrachytherapy
2023 - ROSERO, WILMMER A.A.; BARBEZAN, ANGELICA B.; ROSTELATO, MARIA E.C.M.; SOUZA, CARLA D. de; NOGUEIRA, BEATRIZ R.; ZEITUNI, CARLOS A.
For more than 50 years, the Energy and Nuclear Research Institute IPEN, has been offering solutions to Brazil through nuclear technology. Thus, one of the main areas where IPEN has contributed assertively is medicine. Reaching the level of 32 radiopharmaceuticals and radioactive sources intended both for therapy and for the diagnosis of several pathologies, including cancer, which are obtained with the help of the two nuclear reactors and two cyclotrons present in the institution. The Institute has a team for the development, production and distribution of radioactive sources for brachytherapy, such as 192 Ir wires and 125 I seeds. Brachytherapy is a cancer treatment technique where the radioactive source is placed close to or in contact with the lesion. The great advantage of the technique is to save healthy tissues. Currently, we are working on obtaining nanometric materials that can be applied in the emerging nano brachytherapy, because of its properties and characteristics at the nanometric level, gold has been the subject of studies and tests. Elemental Au gold can be activated 198 Au inside a nuclear reactor, and has β- decay and a half-life of 2.7 days, which makes it ideal for short-term irradiations. In addition, gold in the form of nanoparticles has a completely different chemistry, with gold nanoparticles (AuNPs) being easily functionalized by a large part of molecular and polymeric binders, which may present favorable characteristics for the studies, and together with AuNPs they are able to work synergistically to achieve greater efficiencies. Currently, AuNPs have been successfully functionalized with gum arabic (GA), a coating widely used in the cosmetic and food industry, which is low cost and along with nanoparticles has shown biocompatibility with different cell groups and has been shown to be very stable over time. The project includes studies regarding the synthesis of nanoparticles, coating, cytotoxicity of AuNPs in vitro "cold" (non-radioactive) and the development of activation protocols in the nuclear reactor. In the next phase, after activation, in the reactor, "hot" tests will be performed in vitro and in vivo.
Characterization of FXG dosimeters with gold nanoparticles for brachytherapy applications
2023 - RODRIGUES, PRISCILA; BURIN, ANA; BARBEZAN, ANGELICA; TALACIMON, CRISTHIAN; ILCA, MEDEIROS; TEODORO, LARA E.H.; RIGO, MARIA; GESSERAME, MAYSA; TAVARES, PAULO; NOGUEIRA, THUANY; ROSERO, WILMMER; RODRIGUES JUNIOR, ORLANDO; ZEITUNI, CARLOS; ROSTELATO, MARIA