CAROLINA BENETTI
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Artigo IPEN-doc 29624 Comparison of two light wavelengths (λ = 660 nm and λ = 780 nm) in the repair process of oral mucositis induced by ionizing radiation2023 - ANDRADE, MAIRA F.; SARDO, ARIANE V.N.; BENETTI, CAROLINA; SICCHIERI, LETICIA B.; CORREA, LUCIANA; ZEZELL, DENISE M.Photobiomodulation (PBM) has been clinically used for the prevention and treatment of oral mucositis (OM). The effect of red and near-infrared wavelengths on OM repair is still misunderstood. The aim of this study was to compare the clinical effect and tissue changes caused by 660 nm and 780 nm exposure in an experimental model of OM. Rats were submitted to gamma irradiation for induction of OM lesions and treated with 660 nm or 780 nm lasers with the same dosimetry parameters (30 mW, 7.5 J/cm2, 10 s, spot size = 0.04 mm, irradiation every two days). Clinical assessment of OM severity and histopathological analyses was performed after 8, 14, and 20 days of the ionizing radiation. OM severity was reduced in the PBM groups, especially when the red laser was used. The histopathological pattern was similar between the PBM groups, showing advanced re-epithelization and more pronounced angiogenesis and collagen deposition compared to the control. The 660 nm group showed a greater collagen matrix area than the 780 nm group at 14 days. In conclusion, PBM at 660 nm and 780 nm improved the repair of ionizing radiation-induced OM. Both wavelengths activated the angiogenesis and collagen deposition, but these tissue effects were more pronounced when 660 nm was used.Artigo IPEN-doc 23188 Descriptive analysis of in vitro cutting of swine mitral cusps: comparison of high-power laser and scalpel blade cutting techniques2017 - PINTO, NATHALI C.; POMERANTZEFF, PABLO M.A.; DEANA, ALESSANDRO; ZEZELL, DENISE; BENETTI, CAROLINA; AIELLO, VERA D.; LOPES, LUCIANA A.; JATENE, FABIO B.; CHAVANTES, M.C.Background and objectives: The most common injury to the heart valve with rheumatic involvement is mitral stenosis, which is the reason for a big number of cardiac operations in Brazil. Commissurotomy is the traditional technique that is still widely used for this condition, although late postoperative restenosis is concerning. This study's purpose was to compare the histological findings of porcine cusp mitral valves treated in vitro with commissurotomy with a scalpel blade to those treated with high-power laser (HPL) cutting, using appropriate staining techniques. Materials and methods: Five mitral valves from healthy swine were randomly divided into two groups: Cusp group (G1), cut with a scalpel blade (n = 5), and Cusp group (G2), cut with a laser (n = 5). G2 cusps were treated using a diode laser (lambda = 980 nm, power = 9.0 W, time = 12 sec, irradiance = 5625 W/cm(2), and energy = 108 J). Results: In G1, no histological change was observed in tissue. A hyaline basophilic aspect was focally observed in G2, along with a dark red color on the edges and areas of lower birefringence, when stained with hematoxylin-eosin, Masson's trichrome, and Sirius red. Further, the mean distances from the cutting edge in cusps submitted to laser application and stained with Masson's trichrome and Sirius red were 416.7 and 778.6 mu m, respectively, never overcoming 1mm in length. Conclusions: Thermal changes were unique in the group submitted to HPL and not observed in the cusp group cut with a scalpel blade. The mean distance of the cusps' collagen injury from the cutting edge was less than 1mm with laser treatment. Additional studies are needed to establish the histological evolution of the laser cutting and to answer whether laser cutting may avoid valvular restenosis better than blade cutting.Resumo IPEN-doc 22904 Semi-quantitative analysis in FTIR: exploring and testing four methods for band area calculation in bone2016 - CASTRO, P.A.A.; BENETTI, C.; DIAS, DERLY A.; ZEZELL, D.M.Artigo IPEN-doc 22449 The use of a high-power laser on swine mitral valve chordae tendineae2016 - PINTO, NATHALI C.; CHAVANTES, M.C.; ZEZELL, DENISE; DEANA, ALESSANDRO; BENETTI, CAROLINA; MARCOS, RODRIGO L.; LOPES, LUCIANA A.; MARTINS, A.B.L.; AIELLO, VERA D.; JATENE, FABIO B.; POMERANTZEFF, PABLO M.A.Artigo IPEN-doc 22334 ATR-FTIR spectroscopy to study the effects of laser irradiation in bone tissue2014 - BENETTI, CAROLINA; SANTOS, MOISES O. dos; ANA, PATRICIA A. da; BACHMANN, LUCIANO; ZEZELL, DENISE M.Artigo IPEN-doc 21611 Mid-infrared spectroscopy analysis of the effects of Erbium, Chromium:Yattrium-Scandium-Gallium-Garnet (Er, Cr:YSGG) laser irradiation on bone mineral and organic components2015 - BENETTI, CAROLINA; ANA, PATRICIA A.; BACHMANN, LUCIANO; ZEZELL, DENISE M.Artigo IPEN-doc 21289 FTIR spectroscopy revealing the effects of laser and ionizing radiation on biological hard tissues2015 - ZEZELL, DENISE M.; BENETTI, CAROLINA; VELOSO, MARCELO N.; CASTRO, PEDRO A.A.; ANA, PATRICIA A.Artigo IPEN-doc 20858 In vitro evaluation of ionizing radiation effects in bone tissue by FTIR spectroscopy2015 - ZEZELL, DENISE M.; VELOSO, MARCELO N.; DIAS, DERLY A.; POLITANO, RODOLFO; BENETTI, CAROLINAArtigo IPEN-doc 20860 Optical properties of human radicular dentin: ATR-FTIR characterization and dentine tubule direction influence on radicular post adhesion2015 - QUINTO JUNIOR, JOSE; ZAMATARO, CLAUDIA B.; BENETTI, CAROLINA; DIAS, DERLY A.; BLAY, ALBERTO; ZEZELL, DENISE M.Artigo IPEN-doc 20857 Biochemical changes in cutaneous squamous cell carcinoma submitted to PDT using ATR-FTIR spectroscopy2015 - LIMA, CASSIO A.; GOULART, VIVIANE P.; CASTRO, PEDRO A.A. de; CORREA, LUCIANA; BENETTI, CAROLINA; ZEZELL, DENISE M.Nonmelanoma skin cancers are the most common form of malignancy in humans. Between the traditional treatment ways, the photodynamic therapy (PDT) is a promising alternative which is minimally invasive and do not requires surgical intervention or exposure to ionizing radiation. The understanding of the cascade of effects playing role in PDT is not fully understood, so that define and understand the biochemical events caused by photodynamic effect will hopefully result in designing better PDT protocols. In this study we investigated the potential of the FTIR spectroscopy to assess the biochemical changes caused by photodynamic therapy after 10 and 20 days of treatment using 5-aminolevulinic acid (ALA) as precursor of the photosensitizer photoporphyrin IX (PpIX). The amplitude values of second derivative from vibrational modes obtained with FTIR spectroscopy showed similar behavior with the morphological features observed in histopathological analysis, which showed active lesions even 20 days after PDT. Thus, the technique has the potential to be used to complement the investigation of the main biochemical changes that photodynamic therapy promotes in tissue.