TAIS LIMA DE OLIVEIRA

TAIS LIMA DE OLIVEIRA

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Cycloheximide increases the synthesis of recombinant glycosylated human prolactin (G-hPRL) secreted by CHO cells
2008 - SOARES, C.R.J.; HELLER, S.R.; OLIVEIRA, T.L.; ARTHUSO, F.; GOULART, H.R.; BARTOLINI, P.
The effect of cycloheximide on the glycosylation of recombinant human prolactin synthesized in cho cells
2007 - HELLER, S.R.; OLIVEIRA, T.L.; ARTHUSO, F.S.; SOUSA, J.M.; BARTOLINI, P.; SOARES, C.R.
Bioactive and properly folded prolactin is obtained in E.coli in a pilot bioreactor at 37 graus C by constitutive expression based on the unrepressed ?Psub(L) promoter
2007 - OLIVEIRA, T.L.; HELLER, S.R.; ARTHUSO, F.S.; SOUSA, J.M.; BARTOLINI, P.; SOARES, C.R.
Human prolactin (hPRL) and growth hormone (hGH) distinct behavior under bacteriophage lamdda Psub(L) promoter control
2006 - SOARES, CARLOS R.J.; UEDA, ERIC K.M.; OLIVEIRA, TAIS L.; HELLER, SUSANA R.; BARTOLINI, PAOLO
Distinct human prolactin (hPRL) and growth hormone (hGH) behavior under bacteriophage lambda Psub(L) promoter control: temperature plays a major role in protein yields
2008 - SOARES, C.R.J.; UEDA, E.K.M.; OLIVEIRA, T.L.; GOMIDE, F.I.C.; HELLER, S.R.; BARTOLINI, P.
Synthesis, purification and characterization of recombinant glycosylated human prolactin (G-hPRL) secreted by cycloheximide-treated CHO cells
2010 - HELLER, S.R.; GOULART, H.R.; ARTHUSO, F.S.; OLIVEIRA, T.L.; BARTOLINI, P.; SOARES, C.R.J.
Enhancement of human prolactin synthesis by sodium butyrate addition to serum-free CHO cell culture
2010 - GOULART, HERBERT R.; ARTHUSO, FERNANDA dos S.; CAPONE, MARCOS V.N.; OLIVEIRA, TAIS L. de; BARTOLINI, PAOLO; SOARES, CARLOS R.J.
Sodium butyrate (NaBu) has been used as a productivity enhancer for the synthesis of recombinant proteins in Chinese hamster ovary (CHO) cells. Thus, the influence of NaBu on the production of recombinant human prolactin (hPRL) from CHO cells was investigated for the first time. CHO cell cultures were submitted to a treatment with different concentrations of NaBu (0.25 to 4 mM). Quantitative and qualitative analyses by reverse-phase high-performance liquid chromatography (RP-HPLC) and Western blot or SDS-PAGE, carried out directly on CHO-conditioned medium, showed that the highest hPRL expression was obtained with 1 mM NaBu. In vitro biological assays based on noble rat lymphoma (Nb2) and mouse pro-B lymphoma (Ba/F3-LLP) cells were carried out on purified hPRL. Its bioactivity in the presence of NaBu was not apparently different from that of the First International Reference Reagent of recombinant hPRL (WHO 97/714). Our results show that NaBu increased the synthesis of recombinant hPRL in CHO cells, apparently without compromising either its structure or function.