PEDRO ARTHUR AUGUSTO DE CASTRO
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Artigo IPEN-doc 30700 Differentiation of bone submitted to single dose from fractional of ionizing radiation by ATR-FTIR spectroscopy2024 - GERMANO, G.; CASTRO, P.A.A. de; DIAS, D.A.; VELOSO, M.N.; ZEZELL, D.M.Artigo IPEN-doc 29344 Bone as a biomarker for detecting low dose of ionizing radiation2022 - CASTRO, PEDRO A.A. de; DIAS, DERLY A.; VELOSO, MARCELO N.; ZEZELL, DENISE M.FTIR spectroscopy was able to discriminate bone samples receiving low-dose ionizing radiation doses (0.002 kGy, 0.004 kGy, 0.007 kGy), offering valuable insights in view of the understanding of radiation dose response in biological systems.Artigo IPEN-doc 28871 Assessment of bone dose response using ATR-FTIR spectroscopy2022 - CASTRO, PEDRO A.A. de; DIAS, DERLY A.; DEL-VALLE, MATHEUS; VELOSO, MARCELO N.; SOMESSARI, ELIZABETH S.R.; ZEZELL, DENISE M.The health care application of ionizing radiation has expanded worldwide during the last several decades. While the health impacts of ionizing radiation improved patient care, inaccurate handling of radiation technology is more prone to potential health risks. Therefore, the present study characterizes the bone dose response using bovine femurs from a slaughterhouse. The gamma irradiation was designed into low-doses (0.002, 0.004 and 0.007 kGy) and high-doses (1, 10, 15, 25, 35, 50 and 60 kGy), all samples received independent doses. The combination of FTIR spectroscopy and PLS-DA allows the detection of differences in the control group and the ionizing dose, as well as distinguishing between high and low radiation doses. In this way, our findings contribute to future studies of the dose response to track ionizing radiation effects on biological systems.Resumo IPEN-doc 24937 Biochemical evaluation of bone submitted to ionizing radiation by ATR-FTIR spectroscopy2017 - CASTRO, PEDRO A.A. de; DIAS, DERLY A.; VELOSO, MARCELO N.; ZEZELL, DENISE M.FTIR spectroscopy associated with PC-LDA was able to discriminate bone samples receiving different ionizing radiation doses (0,01 kGy, 1 kGy, 15 kGy), showing potential to the use of phosphate vibrational modes as a dose marker. OCIS codes: (170.4580) Medical optics and biotechnology – Optical diagnostics for medicine; (170.6510) Medical optics and biotechnology – Spectroscopy, tissue diagnostics; (170.1580) Medical optics and biotechnology – Chemometrics.Artigo IPEN-doc 24103 Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra2017 - CASTRO, PEDRO A.A. de; DIAS, DERLY A.; ZEZELL, DENISE M.Ionizing radiation has been used as an important treatment and diagnostic method for several diseases. Optical techniques provides an efficient clinical diagnostic to support an accurate evaluation of the interaction of radiation with molecules. Fourier-transform infrared spectroscopy coupled with attenuated total reflectance (ATR-FTIR) is a label-free and nondestructive optical technique that can recognize functional groups in biological samples. In this work, 30 fragments of bone were collected from bovine femur diaphysis. Samples were cut and polished until 1 cm x 1 cm x 1 mm, which were then stored properly in the refrigerated environment. Samples irradiation was performed with a Cobalt-60 Gammacell Irradiator source at doses of 0.1 kGy, 1 kGy, whereas the fragments exposed to dose of 15 kGy was irradiated in a multipurpose irradiator of Cobalt-60. Spectral data was submitted to principal component analysis followed by linear discriminant analysis. Multivariate analysis was performed with Principal component analysis(PCA) followed by Linear Discriminant Analysis(LDA) using MATLAB R2015a software (The Mathworks Inc., Natick, MA, USA). We demonstrated the feasibility of using ATR-FTIR spectroscopy associated with PCA-LDA multivariate technique to evaluate the molecular changes in bone matrix caused by different doses: 0.1 kGy, 1 kGy and 15 kGy. These alterations between the groups are mainly reported in phosphate region. Our results open up new possibilities for protein monitoring relating to dose responses.Resumo IPEN-doc 22904 Semi-quantitative analysis in FTIR: exploring and testing four methods for band area calculation in bone2016 - CASTRO, P.A.A.; BENETTI, C.; DIAS, DERLY A.; ZEZELL, D.M.Artigo IPEN-doc 20969 Biochemical evaluation of bone submitted to ionizing radiation using ATR-FTIR spectroscopy associated to cluster analysis2015 - DIAS, DERLY A.; VELOSO, MARCELO N.; CASTRO, PEDRO A.A. de; LIMA, CASSIO A.; ZEZELL, DENISE M.