LUCIANA CORRÊA
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Resumo IPEN-doc 29204 Avaliação da área epitelial marcada com CK 10 e CK 14 após fotobiomodulação em mucosite oral induzida por radiação gama2022 - SARDO, A.V.N.; CORRÊA, L.; PRADO, F.C.R.; FIGUEIREDO, A.; ANDRADE, M.F.; ZEZELL, D.M.A mucosite oral (MO) decorre de tratamento quimoterápico e/ou radioterapico em cabeça e pescoço, podendo gerar úlceras que predispõem o paciente a quadros infecciosos e álgicos. A fotobiomodulação (PBM) com laser de baixa intensidade vem sendo destaque nos últimos anos, com bons resultados clínicos. Poucos trabalhos descrevem a ação da PBM na MO em relação ao amadurecimento epitelial. As citoqueratinas 10 e 14 (CK10 e CK14) são exemplos de proteínas que podem auxiliar este raciocínio, sendo a CK 10 característica de queratinócitos com maior grau de diferenciação e a CK14 de queratinócitos em camadas menos diferenciadas. Este trabalho avaliou a extensão da área epitelial marcada por meio de imunohistoquímica, positiva para CK10 e CK14, em MO radioinduzida em língua de ratos e tratada por PBM com laser 660 nm e 789 nm, comparados com grupo não tratado. As amostras foram coletadas nos dias 8 e 20 após a sessão de irradiação. A área expressando CK10 foi estatisticamente maior no grupo tratado com laser vermelho no vigésimo dia do experimento em relação aos demais grupos. Para a CK 14, não houve diferença estatística na quantidade de área marcada, mas sim na distribuição da marcação desta proteína pelo epitélio, que nos grupos tratados com PBM encontrava-se restrita a camada basal e no grupo controle encontrava-se também marcada em camadas mais superiores do epitélio. Conclui-se que a PBM no tratamento de MO com laser 660 nm aumentou a marcação de CK10 e, para ambos os comprimentos de onda, restringiu a marcação da CK14 a extratos basais de epitélio, como é esperado em epitélios normais.Artigo IPEN-doc 22661 Treatment of mucocele of the lower lip with diode laser in pediatric patients: presentation of 2 clinical cases2010 - PEDRON, IRINEU G.; GALLETTA, VIVIAN C.; AZEVEDO, LUCIANE H.; CORREA, LUCIANAMucoceles are common benign lesions of the oral cavity that develop foiiowing extravasation or retention of mucous materiai from salivary glands in the subepitheiial tissue. Most dentai iiterature reports a higher incidence of mucoceie in young patients, with trauma being a leading cause. Treatment may be performed by conventionai surgery, cryotherapy, and, more recently, laser surgery and laser vaporization. The purpose of this report was to describe 2 eiinieai eases of iower-lip mueoeeies treated by exeision with a high-intensity diode iaser in pédiatrie patients. Diode toser surgery was rapid, bioodiess, and weil aeeepted by patients. Postoperative probiems, discomfort, and scarring were minlmaL Treatment of mucoceies with highintensity diode laser provided satisfactory results in the cases presented and allowed for a histopathologicai examination of the excised tissue.Artigo IPEN-doc 21297 Noninvasive monitoring of photodynamic therapy on skin neoplastic lesions using the optical attenuation coefficient measured by optical coherence tomography2015 - GOULART, VIVIANE P.; SANTOS, MOISES O. dos; LATRIVE, ANNE; FREITAS, ANDERSON Z.; CORREA, LUCIANA; ZEZELL, DENISE M.Photodynamic therapy (PDT) has become a promising alternative for treatment of skin lesions such as squamous cell carcinoma. We propose a method to monitor the effects of PDT in a noninvasive way by using the optical attenuation coefficient (OAC) calculated from optical coherence tomography (OCT) images. We conducted a study on mice with chemically induced neoplastic lesions and performed PDT on these lesions using homemade photosensitizers. The response of neoplastic lesions to therapy was monitored using, at the same time, macroscopic clinical visualization, histopathological analysis, OCT imaging, and OCT-based attenuation coefficient measurement. Results with all four modalities demonstrated a positive response to treatment. The attenuation coefficient was found to be 1.4 higher in skin lesions than in healthy tissue and it decreased after therapy. This study shows that the OAC is a potential tool to noninvasively assess the evolution of skin neoplastic lesions with time after treatment.Resumo IPEN-doc 20905 Biochemical changes in normal skin caused by squamous cell carcinoma using FTIR spectroscopy2015 - LIMA, CASSIO; GOULART, VIVIANE; CORREA, LUCIANA; ZEZELL, DENISEArtigo IPEN-doc 20857 Biochemical changes in cutaneous squamous cell carcinoma submitted to PDT using ATR-FTIR spectroscopy2015 - LIMA, CASSIO A.; GOULART, VIVIANE P.; CASTRO, PEDRO A.A. de; CORREA, LUCIANA; BENETTI, CAROLINA; ZEZELL, DENISE M.Nonmelanoma skin cancers are the most common form of malignancy in humans. Between the traditional treatment ways, the photodynamic therapy (PDT) is a promising alternative which is minimally invasive and do not requires surgical intervention or exposure to ionizing radiation. The understanding of the cascade of effects playing role in PDT is not fully understood, so that define and understand the biochemical events caused by photodynamic effect will hopefully result in designing better PDT protocols. In this study we investigated the potential of the FTIR spectroscopy to assess the biochemical changes caused by photodynamic therapy after 10 and 20 days of treatment using 5-aminolevulinic acid (ALA) as precursor of the photosensitizer photoporphyrin IX (PpIX). The amplitude values of second derivative from vibrational modes obtained with FTIR spectroscopy showed similar behavior with the morphological features observed in histopathological analysis, which showed active lesions even 20 days after PDT. Thus, the technique has the potential to be used to complement the investigation of the main biochemical changes that photodynamic therapy promotes in tissue.Artigo IPEN-doc 20832 ATR-FTIR spectroscopy for the assessment of biochemical changes in skin due to cutaneous squamous cell carcinoma2015 - LIMA, CASSIO A.; GOULART, VIVIANE P.; CORREA, LUCIANA; PEREIRA, THIAGO M.; ZEZELL, DENISE M.Nonmelanoma skin cancers represent 95% of cutaneous neoplasms. Among them, squamous cell carcinoma (SCC) is the more aggressive form and shows a pattern of possible metastatic profile. In this work, we used Fourier transform infrared spectroscopy (FTIR) spectroscopy to assess the biochemical changes in normal skin caused by squamous cell carcinoma induced by multi-stage chemical carcinogenesis in mice. Changes in the absorption intensities and shifts were observed in the vibrational modes associated to proteins, indicating changes in secondary conformation in the neoplastic tissue. Hierarchical cluster analysis was performed to evaluate the potential of the technique to differentiate the spectra of neoplastic and normal skin tissue, so that the accuracy obtained for this classification was 86.4%. In this sense, attenuated total reflection (ATR)-FTIR spectroscopy provides a useful tool to complement histopathological analysis in the clinical routine for the diagnosis of cutaneous squamous cell carcinoma.Artigo IPEN-doc 20709 Photodynamic therapy has antifungal effect and reduces inflammatory signals in Candida albicans-induced murine vaginitis2014 - SENA, R.M.M. de; CORREA, L.; KATO, I.T.; PRATES, R.A.; SENNA, A.M.; SANTOS, C.C.; PICANCO, D.A.; RIBEIRO, M.S.Resumo IPEN-doc 20643 Non-invasive monitoring of PDT on skin squamous cell carcinoma using the optical attenuation coefficient measured by OCT2014 - GOULART, VIVIANE P.; SANTOS, MOISES O. dos; CORREA, LUCIANA; LATRIVE, ANNE; LIMA, CASSIO A. de; ZEZELL, DENISE M.Artigo IPEN-doc 20442 Attenuated total reflection fourier transform infrared (ATR-FTIR) spectroscopic analysis of regenerated bone2014 - BENETTI, CAROLINA; KAZARIAN, SERGEI G.; ALVES, MARCO A.V.; BLAY, ALBERTO; CORREA, LUCIANA; ZEZELL, DENISE M.Resumo IPEN-doc 14798 Treatment of oral mucositis induced by ionizing radiation in hamsters with low power laser2008 - GALLETTA, VIVIAN; CORREA, MELISSA F.; CORREA, L.; GOUW SOARES, SHEILA; ZEZELL, D.M.