FABIANE NUNES RIELLO
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Artigo IPEN-doc 28073 The state of the art of theranostic nanomaterials for lung, breast, and prostate cancers2021 - FREITAS, LUCAS F.; FERREIRA, ARYEL H.; THIPE, VELAPHI C.; VARCA, GUSTAVO H.C.; LIMA, CAROLINE S.A.; BATISTA, JORGE G.S.; RIELLO, FABIANE N.; NOGUEIRA, KAMILA; CRUZ, CASSIA P.C.; MENDES, GIOVANNA O.A.; RODRIGUES, ADRIANA S.; SOUSA, THAYNA S.; ALVES, VICTORIA M.; LUGAO, ADEMAR B.The synthesis and engineering of nanomaterials offer more robust systems for the treatment of cancer, with technologies that combine therapy with imaging diagnostic tools in the so‐called nanotheranostics. Among the most studied systems, there are quantum dots, liposomes, polymeric nanoparticles, inorganic nanoparticles, magnetic nanoparticles, dendrimers, and gold nanoparticles. Most of the advantages of nanomaterials over the classic anticancer therapies come from their optimal size, which prevents the elimination by the kidneys and enhances their permeation in the tumor due to the abnormal blood vessels present in cancer tissues. Furthermore, the drug delivery and the contrast efficiency for imaging are enhanced, especially due to the increased surface area and the selective accumulation in the desired tissues. This property leads to the reduced drug dose necessary to exert the desired effect and for a longer action within the tumor. Finally, they are made so that there is no degradation into toxic byproducts and have a lower immune response triggering. In this article, we intend to review and discuss the state‐of‐the‐art regarding the use of nanomaterials as therapeutic and diagnostic tools for lung, breast, and prostate cancer, as they are among the most prevalent worldwide.Resumo IPEN-doc 27661 Screen-printed electrodes functionalization using polimeric matrices2020 - NOTARIO, A.O.; RIELLO, F.N.; FERREIRA, K.d.; MEDEIROS, E.S.; FILHO, L.G.Biosensors are analytical devices able of converting a biological response into a signal of another nature. In electrochemical biosensors electrode functionalization is a fundamental step. The surface of the electrode, where the interaction with the biological sample occur, must be properly treated so that the signal can be captured in the best way possible, without noise interference and for reproducibility. We aim in this work to use polymeric structures, called blanket, to stabilize the surface of screen-printed electrodes. The blankets are composed of hydrophilic and hydrophobic polymers blend enriched with nanomaterials and were manufactured using the solution blow spinning (SBS) technique. The blankets were placed in contact with the electrode surface and the functionalization by polymer deposition was induced through the current flow. Subsequently, the modification was validated from voltammetry readings and impedance spectroscopy. Scanning electron microscopy showed that there was no change in the microscopic surface of the treated electrodes. However, the blankets were able to improve the reading signal, increasing the active area and current flow and homogenizing the readings between the electrodes. These observed effects may be related to a chemical change in the electrodes and not a physical one. The strategy presented here has the advantage that the polymeric matrices are easy to obtain and inexpensive and can be enriched with various materials. Ensuring that the electrode functionalization step is efficient is essential for the construction of a biosensor, as it also ensures that the capture molecules deposit in a similar manner in each repetition. Finally, this standardization step enables new platforms to be built for disease diagnosis and detection of specific targets.Resumo IPEN-doc 27660 Electrochemical immunosensor using magnetic capture for disease diagnosis2020 - RIELLO, F.N.; NOTARIO, A.O.; GOULART, I.B.; GOULART, L.R.Immunosensors are small devices that use biological reactions, relying on antigen-antibody binding to form an immune complex. Methods involving this detection have shown great possibilities for the diagnosis of diseases, but there are still some limitations. In a search for new techniques to increase specific recognition between biomolecules and electrode surface adhesion with faster, lower cost and portability for point-of-care tests, an antibody-coupled magnetic nanoparticle capture system was developed in order to detect antigens in an electrochemical biosensor. Mycobacterium leprae samples were used as an experimental model of more accurate diagnostic tools for this disease. Magnetic iron oxide (Fe3O4) nanoparticles were bioconjugated by covalent binding with M. leprae specific antibody (anti-PGL-I) using EDC promoting direct binding and NHS for stability. Slit-skin smear from leprosy patients with different bacillus concentrations and healthy contacts (negative control) previously quantified by real-time PCR (qPCR / RLEP) were incubated with the bioconjugate and adsorbed on the modified screen-electrode work area. The readings were taken in cyclic voltammetry with portable potentiostat support electrolyte and the PSTouch smartphone software was used to interpret the results. Voltammogram curves have qualitatively discriminated positive from negative samples. Quantitative differences were given by means of logarithmic calculations of the highest values of oxidation peaks in cyclic voltammetry and calibrated based on the number of bacilli previously quantified by qPCR. The novel biosensor presented a detection range from 1 to 1,000,000 bacilli. Briefly, our immunosensor was the first successfully prototype demonstrated for M. leprae detection in direct biological samples from patients. The strategy of magnetic antigen capture proved to be efficient by increasing the sensitivity of the test, because this technique allows the recognition and precipitation of specific antigens. Although it has been used for a specific model, this type of sensor can be applied to different types of diagnostics using antigen and antibody recognition, as the methodology used for bioconjugation is not restricted to the antibody used here. It is also efficient for samples that are difficult to process and where the analyte concentration is low. It is important to emphasize that the new biosensor is portable, fast, sensitive, specific, low-cost and ideal for field screening programs.Resumo IPEN-doc 27644 Synthesis and purification of albumin-based nanoparticles crosslinked by radiation2020 - RIELLO, F.N.; VARCA, G.H.; LIMA, C.S.; FREITAS, L.F.; FERREIRA, A.H.; LUGAO, A.B.Protein-based nanoparticles have been proved a promissing alternative for the loading and delivery of chemotherapeutic agents, radiopharmaceutics and other drugs of interests, constituting a less toxic therapeutic option due to its biocompatibility and low or null side effects. The use of radiation to crosslink or form covalent bonds enables the controll of the crosslinking process, without the need for crosslinking agents, as well as provides sterilizations simultaneously, withouth generating toxic compounds or products. The present work targets the synthesis an purification of albumin-based nanocarrier crosslinked by gamma radiation for biomedical applications. For such purpose, albumin nanoparticles were synthesized using BSA at 20% ethanol (v/v) in 50 mM phosphate buffer on an ice bath prior to and after irradiation. Samples were exposed to gamma radiation at a minimun absrobed dose of 10 kGy at 5kGy.h-1 and purified using a SuperdexTM 200 Increase 10/300GL for isolating the crosslinked protein (high molecular weight) from the native BSA. After the purification, the fractions were characterized by electrophoresis, Uv, fluorescence and dynamic light scaterring. The nanoparticles were obtained in the range of 25-40 nm and purified into fractions of high molecular weight and the native ones. The high molecular weight fractions presented increased bityrosine levels if compared to the fraction corresponded to the native BSA. The yields of nanoparticle formation remains to be determined, but our results provided a clear evidence of the formation of radiation-crosslinked BSA nanoparticles and the role of bityrosine in the nanoparticle assembly.