AECIO MASSAYOSHI YAMADA JUNIOR

AECIO MASSAYOSHI YAMADA JUNIOR

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Bacterial reduction by photodynamic therapy in peri-implantitis
2004 - YAMADA JUNIOR, A.M.; HAYEK, R.R.A.; GIOSO, M.A.; FERREIRA, J.; BATISTA SOBRINHO, C.A.; RIBEIRO, M.S.
Progressive peri-implantar bone losses, which are accompanied by inflammatory process in the soft tissues is referred to as peri-implantitis. The aim of this study was to compare the effects of lethal photosensitization with the conventional technique on bacterial reduction in ligature induced peri-implantitis in dogs. Seventeen third premolars of eight Labrador dogs were extracted and, immediately after, the implants were submerged. After osseointegration, peri-implantitis was induced. After 4 months, ligature were removed and the same period was waited for natural induction of bacterial plaque. The dogs were randomly divided into two groups. In the conventional group, they were treated with the conventional techniques of mucoperiosteal flaps for scaling the implant surface and irrigation with chlorexidine. In the laser group, only mucoperiosteal scaling was carried out before photodynamic therapy. On the peri-implantar pocket an azulene paste was introduced and a GaAlAs low-power laser (l= 660 nm, P= 30 mW, E= 5,4 J and Dt= 3 min.) was applied. Microbiological samples were obtained before and immediately after treatment. The results of this study showed that Prevotella sp., Fusobacterium e S. Beta-haemolyticus were significantly reduced for the conventional and laser groups (100%,99.8%; 100%,100%; 85.7%,97.6%, respectively).
Comparation between photodynamic therapy and a bactericidal solution in the treatment of dental alveolitis microbiological evaluation
2004 - HAYEK, R.R.A.; YAMADA JUNIOR, A.M.; GARCEZ, A.S.; NUNEZ, S.C.; SUZUKI, L.C.; RIBEIRO, M.S.
Photodynamic therapy is a new therapeutic modality to treat microbial infections. The aim of this pilot study was to compare this new therapeutic approach with clorhexidine in mini pigs induced alveolitis. Alveolitis is an inflammatory disease that involves the superficial bone layer in the dental alveolus. Eight dental elements were extracted from mini pigs and immediately after the alveolus were contaminated with Streptoccocus aureus. After seven days the area presented edema and hyperemia. In the clorhexidine group the alveolus were debrided and irrigated with a 0.12% solution of clorhexidine. In Laser group the alveolus were debrided and photodynamic therapy was performed consisting in the application of a azulene paste inside the alveolus with a preirradiation time of 5 minutes followed by irradiation with a low power diode laser, ?= 685 nm, P= 50 mW, E= 9 J, for 3 minutes. Microbiological samples were harvested before and after treatment for both groups with sterile paper points. The results showed bacterial reduction in both groups, although photodynamic therapy was significantly more effective to reduce the population of S. aureus. This finding indicates that photodynamic therapy can be an alternative method to the treatment of alveolitis.
Terapia fotodinamica: mecaniscos e aplicacoes
2010 - PRATES, RENATO A.; YAMADA JUNIOR, AECIO M.; HASHIMOTO, MARIA C.; RIBEIRO, MARTHA S.
Photosensitization of Aggregatibacter actinomycetemcomitans with methylene blue: A microbiological and spectroscopic study
2008 - YAMADA JUNIOR, AECIO; PRATES, RENATO A.; CAI, SILVANA; RIBEIRO, MARTHA S.
The aim of this study was to determinate the efficiency of methylene blue (MB) to kill cultures of Aggregatibacter actinomycetemcomitans under red light and to investigate MB photobleaching by optical absorption spectroscopy. Bacteria were diluted in aqueous solution, putted in glass tubes and distributed in 5 groups: (L-MB-) control group; (L+MB-) laser alone by 5min; (L-MB+) MB alone through 5min; (3L+MB+) MB+laser 3min; (5L+MB+) MB+laser 5min. Laser parameters were P=30mW, λ=660nm, E=9J in 5min and E=5.4J in 3min. The samples were diluted and bacterial colonies were counted and converted into colony forming units (CFU). Absorption spectra of the MB-stained bacterial suspension and photosensitized bacterial suspension were obtained. Groups L-MB-, L+MB-, and L-MB+ did not show a decrease in CFU/mL. L+MB+ groups showed a significant decrease in CFU/mL but no statistically significant differences were observed between 3min and 5min. Spectroscopy showed that MB is photodegraded after irradiation and that dimer species are more notably consumed than monomeric species. These results suggest that MB is a suitable photosensitizer to reduce A. actinomycetemcomitans, and that 3min of irradiation are enough to produce a significant effect. Due to the spectral changes observed on MB solution after irradiation a type I mechanism may be involved.
Histomorphometric and microbial assessment of photodynamic therapy as an adjuvant treatment for periodontilis: A short-term evaluation of inflammatory periodontal conditions and bacterial reduction in a rat model
2011 - PRATES, RENATO A.; YAMADA JUNIOR, AECIO M.; SUZUKI, LUIS C.; FRANÇA, CRISTIANE M.; CAI, SILVANA; MAYER, MARCIA P.A.; RIBEIRO, ADRIANA C.; RIBEIRO, MARTHA S.