ANDRE GUSTAVO TEMPONE CARDOSO
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Resumo IPEN-doc 07994 L-amino acid oxidase activity from Bothrops moojeni venom on Leishmania (Leishmania) amozonensis promastigotes1998 - TEMPONE, A.G.; LOURENCO, C.O.; SPENCER, P.J.; ANDRADE JUNIOR, H.F.; NASCIMENTO, N.Resumo IPEN-doc 07996 Growth inhibition of Leishmania (Leishmania) amazonensis by Australian snake venoms1998 - LOURENCO, C.O.; TEMPONE, A.G.; SPENCER, P.J.; ANDRADE JUNIOR, H.F.; MIRTSCHIN, P.J.; NASCIMENTO, N.Resumo IPEN-doc 08002 Bothrops moojeni venom action on Leishmania (Leishmania) amazonensis promastigotes1998 - ROCHA, D.C.A.; TEMPONE, A.G.; LOURENCO, C.O.; TAKAKURA, C.F.; ANDRADE JUNIOR, H.F.; NASCIMENTO, N.Artigo IPEN-doc 06602 Gamma radiation affects the anti-Leishmania activity of Bothrops moojeni venom and correlates with L-amino acid oxidase activity1999 - TEMPONE, A.G.; LOURENCO, C.O.; SPENCER, P.J.; ROGERO, J.R.; ANDRADE JUNIOR, H.F.; NASCIMENTO, N.Dissertação IPEN-doc 06648 Artigo IPEN-doc 11423 Synthesis and antieshmanial activities of novel 3 substituted quinolines2005 - TEMPONE, ANDRE G.; SILVA, ANA C.M.P.; BRANDT, CARLOS A.; MARTINEZ, FERNANDA S.; BORBOREMA, SAMANTA E.T.; SILVEIRA, MARIA A.B.; ANDRADE JUNIOR, HEITOR F.Artigo IPEN-doc 11368 Antiprotozoal activity of brazilian plant extracts from isoquinoline alkaloid-producing families2005 - TEMPONE, A.G.; BORBOREMA, S.E.T.; ANDRADE JUNIOR, H.F.; GUALDA, N.C.A.; YOGI, A.; CARVALHO, C.S.; BACHIEGA, D.; LUPO, F.N.; BONOTTO, S.V.; FISCHER, D.C.H.Artigo IPEN-doc 10477 Targeting Leishmania (L.) chagasi amastigotes trough macrophage scavenger receptors: the use of drugs entrapped in liposomes containing phosphatidylserine2004 - TEMPONE, A.G.; PEREZ, D.; RATH, S.; VILARINHO, A.L.; MORTARA, R.A.; ANDRADE JUNIOR, H.F.Artigo IPEN-doc 09773 Antimoniais empregados no tratamento da leishmaniose: estado da arte2003 - RATH, S.; TRIVELIN, L.A.; IMBRUNITO, T.R.; TOMAZELA, D.M.; JESUS, M.N.; MARZAL, P.C.; ANDRADE JUNIOR, H.F.; TEMPONE, A.G.Artigo IPEN-doc 08326 Bothrops moojeni venom kills Leishmania spp. with hydrogen peroxide generated by its L-amino acid oxidase2001 - TEMPONE, A.G.; ANDRADE JUNIOR, H.F.; SPENCER, P.J.; LOURENCO, C.O.; ROGERO, J.R.; NASCIMENTO, N.Leishmaniasis is an endemic tropical disease in South America, with few therapeutic approaches. Snake venoms are complex protein mixtures with biological actions that could be used as tools for drug development. Here we show that Bothrops moojeni crude venom presented a killing effect in vitro against Leishmania spp. promastigotes, but not with amastigotes, as determined by a viability assay using the mitochondrial oxidative function. Purification of active fractions from crude venom was performed by molecular exclusion and ion exchange chromatography. Anti-Leishmania and L-amino acid oxidase (L-AAO, EC.1.4.3.2.) activities co-eluted in the same fractions. The molecular weight of the active enzyme was estimated to be 140 kDa by molecular exclusion chromatography, and 69 kDa by SDS-PAGE, with a 4.8 isoelectric point. Using substrate subtraction and catalase for scavenging, the action of L-AAO was demonstrated to be hydrogen-peroxide-dependent.