ANNA LUCIA CASANAS HAASIS VILLAVICENCIO

Resumo

Anna Lucia C. H. Villavicencio has a degree in Biological Sciences from the Santa Ursula University (1979) at Rio de Janeiro State; Master Degree in Basic Nuclear Technology in the area of Radiobiology, University of São Paulo at the Institute of Energy and Nuclear Research (USP/IPEN), São Paulo State (1989); Doctorate in Food Science, Bromatology and Experimental Nutrition area at the Faculty of Pharmaceutical Sciences, University of São Paulo (1998), São Paulo State; Post-doctorate in searching about the influence of the radiation process on bioactive compounds of eatable flowers in the chemistry and biochemistry laboratories of the Agriculture School of the Polytechnic Institute of Bragança, Portugal, fellow CNPq (2015). CNPq productivity scholarship (level 2). She is professor of the graduation program of IPEN / USP, guides and teaches courses in Nuclear Technology post graduation program at the University of São Paulo (TNA-IPEN / USP), was member of the Commission of Graduate of Nuclear Technology Program (IPEN / USP, 2013-2015). Technologist position at National Nuclear Energy Commission (CNEN) and researcher at the Institute of Energy and Nuclear Research (IPEN-CNEN / SP). She has developed work aimed at Peaceful use of Nuclear Technology Application in support of Food Security in the field of Food Science and Technology, with emphasis on Food Irradiation and Detection Analysis, acting on the following themes: Food Irradiation, Food Detection source Irradiated animal and plant; Analysis Physical Chemistry, Biochemistry, Microbiological and Nutritional Irradiated Food, Assessment and Application of Quarentenário and Phytosanitary treatment in conjunction with the irradiation process in agricultural products; Analysis of Natural biocompounds and radiation processing; Sensorial analysis of irradiated foods; Food Safety and Public Acceptance of Studies Processed Food Radiation; National and international legislation on the use of radiation in food and agricultural products with ABNT; Food detection Transgenic and Biological Effects of Radiation. It has experience in coordinating national and international projects funded by the International Atomic Energy Agency (IAEA), Vienna, Austria, on the interaction of radiation in food. Participates in committees and short courses in theme update related to the irradiation of food and agricultural products. (Text obtained from the Currículo Lattes on October 4th 2021)


Possui licenciatura em Ciências Biológicas pela Universidade Santa Úrsula (1979), mestrado em Tecnologia Nuclear básica na area de Radiobiologia pela Universidade de São Paulo, Instituto de Pesquisas Energéticas e Nucleares (1989); doutorado em Ciências dos Alimentos, área de Bromatologia pela Faculdade de Ciências Farmacêuticas da Universidade de São Paulo (1998) e pós-doutorado no laboratório de química e bioquímica aplicada da Escola de Agricultura do Instituto Politécnico de Bragança, Portugal, bolsista CNPq (2015). Bolsista de produtividade do CNPq (nível 2). É professora titular do programa de pós-graduação do IPEN/USP, orienta e ministra disciplinas no programa de Tecnologia Nuclear da Universidade de São Paulo (TNA-IPEN/USP), foi membro da Comissão de Pós Graduação do Programa de Tecnologia Nuclear (IPEN/USP, 2013-2015). Tecnologista da Comissão Nacional de Energia Nuclear (CNEN) e pesquisadora do Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP). Tem desenvolvido trabalhos visando a Aplicação Pacífica do uso da Tecnologia Nuclear em prol da Segurança Alimentar na área de Ciência e Tecnologia de Alimentos, com ênfase em Análise de Alimentos Irradiados, atuando principalmente nos seguintes temas: Irradiação de Alimentos, Detecção de Alimentos Irradiados de origem animal e vegetal; Análises Físico-Química, Bioquímica, Microbiológica e Nutricional de Alimentos Irradiados, Avaliação e Aplicação do tratamento Quarentenário e Fitossanitário em conjunto com o processo de irradiação em produtos agrícolas; Análise de Biocompostos Naturais e o processamento por radiação; Análise Sensorial de alimentos irradiados; Segurança Alimentar e Estudos de Aceitação Pública de Alimentos Processados por Radiação; Legislação Nacional e Internacional sobre aplicação da Radiação em Alimentos e Produtos Agrícolas junto à ABNT; Detecção de Alimentos Transgênicos e Efeitos Biológicos da Radiação. Tem experiência em coordenar projetos nacionais e internacionais financiados pela Agência Internacional de Energia Atômica (IAEA), Viena, Áustria, sobre a interação da radiação em alimentos. Participa em comissões e cursos de curta duração na atualização do tema relacionado à irradiação de alimentos e produtos agrícolas. (Texto extraído do Currículo Lattes em 04 out. 2021)

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  • Artigo IPEN-doc 29781
    La interdisciplinariedad en la educación básica
    2022 - LEVY, D.; LEVY, P.J.; VILLAVICENCIO, A.L.C.H.
    La Base Nacional Común Curricular (BNCC) es un documento de carácter normativo que define el conjunto de aprendizajes esenciales, competencias y habilidades de la escolaridad básica para todas las escuelas brasileñas. El documento que define las etapas de la educación infantil y enseñanza fundamental fue homologado en 2017, y en 2018 se aprobaron las etapas para la Enseñanza Secundaria. La BNCC contempla cuestiones relacionadas con las ciencias nucleares, buscando el desarrollo de habilidades y competencias para una comprensión justa sobre reacciones nucleares y sus aplicaciones. Sin embargo, los propios profesores no fueron preparados para discutir el tema con profundidad y, generalmente, el tema se aborda de forma superficial a fin de cumplir el contenido mínimo requerido. El presente trabajo presenta el desarrollo de una propuesta pedagógica interdisciplinaria pasible de ser incorporada en la Enseñanza Básica, de forma transversal y integradora, desmitificando la irradiación de alimentos y la generación de energía nuclear, estes controvertidos temas que afectan a la vida humana en escala local, regional y global. El material consiste en una colección de libros ilustrados para los estudiantes, libros para docentes y un manual con actividades que se pueden desarrollar en diferentes disciplinas: portugués, matemáticas, ciencias, inglés, historia y geografía. Los libros son elaborados con alto rigor científico y articulan la tecnología nuclear con temas de interés a la sociedad en los más diversos aspectos, como el social, sanitario y económico, entre otros. El objetivo de esta propuesta pedagógica, a ser sometida al Ministerio de Educación de Brasil, es auxiliar a profesores y estudiantes en la construcción colectiva del conocimiento, incentivando la investigación de las ciencias nucleares, imprimiendo nuevos sentidos a la vida cotidiana, ampliando los horizontes de elecciones personales y profesionales de las nuevas generaciones.
  • Livro IPEN-doc 29577
    A mega master interessante vida de uma pastilha de urânio
    2022 - LEVY, DENISE; VILLAVICENCIO, ANNA L.
  • Livro IPEN-doc 29576
    A mega master interessante vida de uma manga irradiada
    2022 - LEVY, DENISE; VILLAVICENCIO, ANNA L.
  • Artigo IPEN-doc 27057
    Evaluation of cytotoxicity through MTS test of 2-ACBs (2-dDCB AND tDCB) after exposure to hepatic cells
    2019 - BARBEZAN, A.B.; CARVALHO, L.R.; VIEIRA, D.P.; MACHADO-SANTELLI, G.M.; MARTINS, R.; VILLAVICENCIO, A.L.C.H.
    Food irradiation has been approved in more than 60 countries for many applications in a wide variety of foods. 2-Alkylcyclobutanones (2-ACBs) are the only known radiolytic products formed when foods containing fatty acids are irradiated. Despite the importance of food irradiation, the toxicological potential of 2-ACBs in irradiated food is still no fully understood. In this study we investigated the cytotoxic effects of irradiated palmitic and stearic fatty acids byproducts, 2-dDCB and 2-tDCB, in hepatic cell lines (HepG2, BRL3A and HTC). The cytotoxic effects of 2-dDCB and 2-tDCB were evaluated at 100, 300 and 500 μM for 24 and 48 hours and the cell viability was measured using the MTS assay. While no toxicity was observed for 2-tDCB in all cells for all tested conditions, 2-dDCB was found to be toxic to BRL3A cells (at 100 μM after 48 hours) and HTC cells (at 24 hours in all tested concentrations). HepG2 cells on the other hand, were found to be resistant to 2-dDCB-induced toxicity. Overall our data shows that the byproduct 2-tDCB is not toxic for hepatic cells while 2-dDCB can be used has a potential marker for food irradiation-induced toxicity.
  • Resumo IPEN-doc 24769
    Utilization of DNA comet assay and half embryo test to identify irradiated lentil
    2001 - ROMANELLI, MARIA F.; VILLAVICENCIO, ANNA L.C.H.
    Legumes make an important contribution to human nutrition on a worldwide basis. Insect infestation cause extensive damage to stored grains. Over the last few decades some countries adopted food irradiation as a safe food process. Radiation's processing on foods improves hygienic quality and extends their shelf life. The use of radiation treatment to reduce the microbial population and thereby extend the shelf life in legumes has been reported in many papers. Irradiation has been shown to be an effective pest control method for these commodities and a good alternative to prohibited methyl bromide. Radiation disinfestation can facilitate trade in foods that often harbor insect pests of quarantine importance. Although the wholesomeness of irradiated food is no longer a question there is a need for irradiation control in the international trade of foods, in order to enhance the consumer confidence in the regulation. As a screening methods to identify irradiated lentils, processed by e-beam as a food treatment to disinfestation, the DNA Comet Assay and Half Embryo tests were performed. The methodologies used in this work are based upon biological changes that occur in Brazilian lentils. The samples were irradiated in an electron beam accelerator facility of Radiation Dynamics Inc., USA (E=1,5 MeV, l=25 mA). The irradiation doses were 0,7; 1,4 and 3,0 kGy at dry conditions. The thickness of samples was less than 0,5 cm. A sensitive technique to detect DNA fragmentation is the microgel electrophoresis of single cells or nuclei, also called 'comet assay'. Since the large molecule of DNA is an easy target for ionizing radiation, changes in DNA offer potential as a detection method. It is restricted to foods that have not been subjected to heat or other treatments, which also cause DNA fragmentation. Lentil samples were crushed with a mortar and pestle and was transferred to 3ml ice-cold PBS. This suspension was stirred for 5 minutes and filtered. 100μl cell suspension was mixed with 500μl of low-melting agarose (0,8% in PBS). 100μl of this mixture was spread on pre-coated slides. The slides were immersed in lysis buffer (0,045M TBE, pH 8.4, containing 2,5% SDS) for 15 minutes. Electrophoresis was carried out using the same TBE buffer, but devoid of SDS, at a potential of 2V/cm for 2 minutes. Silver staining was carried out for I hour following fixing. Duplicate measurements for each sample were carried out and 100 cells were counted for each dose level. The migration patterns of DNA was evaluated with a standard microscope. Germination tests were carried out in the irradiated and non-irradiated dry lentils, which allows observing characteristically variations on the shoots and roots. The half-embryo test is based on the inhibition of shooting in seeds or grains due to irradiation. It is characterized by its easy detection and sensitivity. The shoots and roots wore observed during 5 days of culturing period under specified conditions. The difference observed in this variety was analyzed only after irradiation treatment at room temperature. Irradiated half-embryo showed markedly reduced root grows and almost totally retarded shoot elongation. Differences between irradiated and non-irradiated half-embryo could be observed.
  • Resumo IPEN-doc 24746
    Chromatographic analysis of irradiated medicinal herbs
    2001 - KOSEKI, PAULA M.; RELA, PAULO R.; VILLAVICENCIO, ANNA L.C.H.
    Introduction: Nowadays the interest in phytotherapeutics is increasing; therefore the consumer attention to the medicinal active plants is growing. The rich Brazilian flora, represents more than 20% of the plant species know in the world as raw materials for pharmaceutical preparations. Since the last decade microbiological decontamination of medicinal herbs by irradiation has been carried out and presented in many scientific articles. The microbial contamination in these raw plant materials is the issue of several studies, which propose appropriate techniques for the reduction of micro-organisms. One of these techniques is radiation processing by gamma source industrial plants. Other is the utilisation of accelerators. In order to safeguard consumers, treatment by ionizing radiation is allowed now in Brazil to medicinal herbs and pharmaceutical products. The radiation process is known as safe for a large variety of products and applications as well as a effective in the reduction of pathogenic micro-organisms. The aim of our study is observe if flavonoids and alkaloids will be influenced by irradiation. Experimental: Samples - Local herbs companies in Sao Paulo, Brazil, provided dehydrated samples of Rhamnus purshiana D.C. and Paulinia cupana Kunth. Irradiation. The powdered samples were irradiated in on plastic package in a electron beam accelerator facility of Radiation Dynamics Inc., USA (E=1,5 MeV, l=25 mA, installed in IPEN Sao Paulo, Brazil. The irradiation doses were 10,20 and 30 kGy at room temperature. The thickness of samples was less than 0,5 cm. Sample analysis -flavonoids and alkaloids analysis was performed in a Thin Layer Chromatography according to Wagner (1995). Results and discussion: No alterations in the flavonoids and alkaloids, after irradiation treatment in that herbs was observed. Chromatographic analysis of the different extracts irradiated at increasing doses indicated that there were no great differences in the chemical constitution of the herbs. The extracts, presented no change in the color increasing the irradiation doses (results are similar in other herbs extracts) as showed in the absorption peaks. The samples showed similar curves, presenting no changes in the chemical compounds. Pharmacological activity of medicinal herbs has been found satisfactory after treatment by high doses radiation.
  • Resumo IPEN-doc 23990
    DNA comet assay as a rapid detection method of irradiated bovine meat by electron beam
    2001 - MARIN-HUACHACA, NELIDA S.; VILLAVICENCIO, ANNA L.C.H.
    Introduction: The presence in food of pathogenic microorganisms, such as Salmonella species, Escherichia coli 0157:H7, Listeria Monocytogenes or Yersinia enterolitica, is a problem of growing concern to public health authorities all over the world. Thus, irradiation of certain prepackaged meat products such as ground beef, minced meat, and hamburgers may help in controlling meatborne pathogens and parasites. Pathogenic microorganisms and parasites in meat products, which are commonly consumed raw, are of particular importance, Up to now, only electron-beam accelerators and gamma-ray cells have been used for commercial applications. At the international conference on 'The Acceptance, Control of, and Trade in Irradiated Food', it was recommended that governments should encourage research into detection methods (Anon, 1989), Already five international standards are available to food control agencies. A number of physical, chemical, and biological techniques of detection of irradiated foods have been discussed in the literature. A rapid and inexpensive screening test employing DNA Comet Assay to identify radiation treatment of food has been described by Cerda et al. (1997). This method is restricted to foods that have not been subjected to heat or other treatments, which also induce DNA fragmentation. Advantages are its simplicity, low cost and speed of measurement. This method was proposed to the European Committee for Standardization (CEN) as a screening protocol (presumptive) and not as a proof (definitive). The DNA comet assay have been yielded good results with chicken, pork, fish meat, exotic meat, hamburgers, fruits and cereals. In this work we studied a DNA fragmentation of bovine meat irradiated by electron beam. Experimental: Bovine meat was purchased in local shops in Sao Paulo. Irradiation was performed with electron beam of accelerator facility of Radiation Dynamics Inc., USA (E=1,5 MeV, l=25 mA). The irradiation doses were 3,5; 4,5, 5,5, and 7,0 kGy at chilled conditions, and 3,5,4,5; 6,0; 7,0 and 8,0 kGy at frozen conditions. The thickness of meat was less than 0,5 cm. Briefly, meat samples were crushed with a mortar and pestle and was transferred to 1ml ice-cold PBS. This suspension was stirred for 5 minutes and filtered. 100μl cell suspension was mixed with 500μl of low-melting agarose (0,8% in PBS). 100μl of this mixture was spread on pre-coated slides. The caste slide were immersed in lysis buffer (0.045M TBE, pH 8.4, containing 2,5% SDS) for 15 minutes. Electrophoresis was carried out using the same TBE buffer, but devoid of SDS, at a potential of 2V/cm for 2 minutes. Silver staining was carried out for 20 minutes following fixing. Duplicate measurements for each sample were carried out and 100 cells were counted for each dose level. The migration patterns of DNA was evaluated with a standard microscope. Results and Discussion: In the work with Comet Assay, increasing DNA degradation was characterized by different migration patterns of DNA, Some differences were observed between chilled and frozen conditions. The storage time influenced the DNA degradation. Conclusions: It was concluded that the comet assay could be used for detection of processing of irradiated bovine meat by electron beam. Also, this method could be used as a freshness indicator.
  • Artigo IPEN-doc 20147
    Gamma radiation as a potential phytosanitary squash treatment for the control of Anastrepha grandis (Macquart) (Diptera: Tephritidae)
    2021 - BALDO, FERNANDO B.; VILLAVICENCIO, ANNA L.C.H.; SCHMIDT, FLAVIO L.; RAGA, ADALTON
    Fruit flies (Diptera: Tephritidae) are the primary pests of horticultural crops worldwide, causing both direct and indirect damage to the fruit production, including Anastrepha grandis (Macquart, 1846), which exclusively infests Cucurbitaceae fruit. Dose-response tests were used to examine the effects of gamma radiation exposure on 24 – 48 h old eggs and third instar larvae, both in vitro and in Atlas squashes (Cucurbita moschata). The following physicochemical properties of Atlas squashes exposed to gamma radiation were evaluated: titratable total acidity, sugar, pH, external colouration, and texture. It was found that under in vitro conditions, a 20.0 Gy dose of gamma radiation was able to prevent larval eclosion, but a 200 Gy dose was necessary to prevent emergence after the exposure of third instar larvae. In Atlas squashes, 200 and 250 Gy doses of gamma radiation were able to prevent the emergence of A. grandis adults from eggs and larvae, respectively. The radiation doses estimated by Probit 9 to prevent emergence from squashes infested with eggs and third instar larvae were 257.13 Gy and 270.25 Gy respectively. The use of gamma radiation did not significantly affect the physicochemical properties of Atlas squashes, which suggested that gamma radiation may represent a potentially useful technique for the quarantine control of A. grandis in cucurbits.
  • Capítulo IPEN-doc 28542
    Irradiação de alimentos
    2021 - VILLAVICENCIO, ANNA L.
  • Artigo IPEN-doc 28257
    Physical properties of irradiated Sapodilla (Manilkara zapota)
    2021 - NEGRAO, B.G.; KOIKE, A.C.R; LANA, J.G.; VILLAVICENCIO, A.L.C.H.