CARLOS HENRIQUE CAMILLO DA SILVA
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Resumo IPEN-doc 29573 FTIR imaging on glass substrates evaluation of histological skin burn injuries specimens treated by femtosecond laser pulses2022 - ZEZELL, DENISE; CASTRO, PEDRO; DEL-VALLE, MATHEUS; CAMILLO-SILVA, CARLOS; SAMAD, RICARDO; DE ROSSI, WAGNER; SANTOS, MOISESBurn injuries continue to be one of the leading causes of unintentional death and injury in low- and middle-income countries [1]. Burns are considered an important public health problem, because in addition to physical problems that can lead the patient to death, they cause psychological and social damage. An estimated 180,000 deaths every year are caused by burns [2]. The use of infrared (IR) spectroscopy for studying biological specimens is nowadays a wide and active area of research. The IR microspectroscopy has proved to be an ideal tool for investigating the biochemical composition of biological samples at the microscopic scale, as well as its fast, sensitive, and label-free nature [3]. IR image spectral histopathology has shown great promise as an important diagnostic tool, with the potential to complement current pathological methods, reducing subjectivity in biopsy samples analysis. However, the use of IR transmissive substrates which are both fragile and prohibitively very expensive, hinder the clinical translation. The goal of this study is to evaluate the potential of discriminating healing process, in burned skin specimens treated with ultrashort pulses laser 3 days after the burn. This study is considering a previous paper [4], in which it analyzed only micro-ATR-FTIR spectra of a frozen sample point. The specimens were obtained from third degree burn wound. The wounds treatment were performed three days after the burn, and the animals were sacrificed 3 and 14 days post-treatment. Using coverslipped H&E stained tissue on glass from previous histopathological analysis and applying the analytical techniques PCA and K-means on N−H, O−H, and C−H stretching regions occurring at 2500−3800 cm−1 (high wavenumber region), were possible to discriminate burned epidermal and dermal regions from irradiated in same regions on sample. In the figures is shown the average spectrum at (a) day 3 and (b) day 14. , in both there were increase of burned+laser treated bands. The great potential of this study was to analyse coverslipped H&E stained tissue on glass, without compromising the histopathologist practices and contribute for clinical translation.Resumo IPEN-doc 29571 Burn wound healing by infrared spectroscopy imaging2022 - CASTRO, P.; SILVA, C.; ZEZELL, D.Aim: Burns are one of the major causes of morbidity and the most costly traumatic injuries worldwide1. The traditional techniques used to assess the biochemical events related to wound repair are laborious, time-consuming and require multiple staining. Thus, the present study aims to evaluate the feasibility of Fourier transform infrared (FTIR) spectroscopy in order to monitor the progress and healing status of burn wounds. Methods : Third-degree burn injuries were induced on Wistar rats by water vapor exposure. Afterwards, biopsies specimen was extracted for further histopathological examination and IR imaging evaluation at 7 days. The spectral imaging was performed using a micro- FTIR equipment in transflection mode (MirrIR, Kevley Technologies), with a 32 x 32 FPA of 5.5 μm pixel size. All spectra data were baseline corrected and vector normalized. The preprocessing and image comparison was performed using Cytospec software (Cytospec, version 2.00.5)2. Results : The pairwise analysis was evaluated in the wavenumber region maps of 1200-1300 cm-1. The biomolecule band associated to collagen content (1200 – 1300 cm-1) was most prominent3 on the 7th day image in the post-burn that in healthy group. Conclusions : Therefore, our pairwise comparison revealed that metabolic activity induced by thermal injury increases the chemical activity associated to the healing progresses. Our findings show that FTIR spectroscopy has potential to identify the biochemical signatures induced by burn injury.Resumo IPEN-doc 27518