SABINE NEUSATZ GUILHEN

Resumo

Possui graduaĆ§Ć£o em QuĆ­mica com atribuiƧƵes TecnolĆ³gicas e BiotecnolĆ³gicas pelo Instituto de QuĆ­mica da Universidade de SĆ£o Paulo (2005), mestrado (2009) e doutorado (2018) em Tecnologia Nuclear (Materiais) pelo Instituto de Pesquisas EnergĆ©ticas e Nucleares (IPEN), Universidade de SĆ£o Paulo. Tem experiĆŖncia em QuĆ­mica AnalĆ­tica com ĆŖnfase em AnĆ”lise de TraƧos, atuando principalmente no desenvolvimento de mĆ©todos analĆ­ticos empregando tĆ©cnicas espectrofotomĆ©tricas (AAS, ICP OES e ICP-MS) para caracterizaĆ§Ć£o de amostras ambientais, arqueolĆ³gicas, biolĆ³gicas, forenses e nucleares. Atualmente, ocupa o cargo de Tecnologista em "CaracterizaĆ§Ć£o QuĆ­mica" no Centro de QuĆ­mica e Meio Ambiente (CQMA) do IPEN (CNEN/SP), onde desempenha atividades de pesquisa e desenvolvimento tecnolĆ³gico em atendimento Ć s demandas institucionais ligadas ao Ciclo do CombustĆ­vel Nuclear e aos Programas de Pesquisa de carĆ”ter multidisciplinar, em apoio a projetos de InovaĆ§Ć£o TecnolĆ³gica e ao Programa de PĆ³s-GraduaĆ§Ć£o do IPEN/USP. AlĆ©m disso, atua na geraĆ§Ć£o de produtos tecnolĆ³gicos e no desenvolvimento de materiais adsorventes de baixo custo e alto valor agregado visando o aproveitamento de materiais e resĆ­duos naturais e/ou renovĆ”veis no tratamento de efluentes e rejeitos. (Texto extraĆ­do do CurrĆ­culo Lattes em 4 maio 2023)

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Resultados de Busca

Agora exibindo 1 - 2 de 2
  • Artigo IPEN-doc 27194
    Variations in essential elements after malignant transformation of kidney epithelial tubular cells
    2020 - BELLINI, MARIA H.; SOUZA, ALEXANDRE L. de; SILVA, FABIO F. da; GUILHEN, SABINE N.; FERREIRA, RAFAEL V. de P.; ARAUJO, LEANDRO G. de; OLIVEIRA, JOAO E. de; MARUMO, JULIO T.
    Cell line-based research is a valuable tool for the study of cancer physiopathology and the discovery of new drugs for use in clinical practice. In this study, inductively coupled plasma mass spectrometry (ICP-MS) was used to estimate Ca, Co, Cu, Fe, K, Mg, Mn, Na, P, S, Se, and Zn in epithelial tubular cells (HK-2) and kidney tumor cells (Caki-1 cells). The most relevant difference was a decrease in the contents of Ca, Cu, Fe, K, Mg, Mn, Na, P, S, and Zn. A significant accumulation of Co was also detected in Caki-1 cells. The fold change variation of each element concentration between HK-2 and Caki-1 cells was Ca (ā€’0.40), Co (1.37), Cu (ā€’0.68), Fe (ā€’0.56), K (ā€’0.40), Mg (ā€’0.41), Mn (-0.54), Na (ā€’0.33), P (ā€’0.31), S (ā€’0.26), and Zn (ā€’0.73). These findings indicate that the elements mainly affect the metabolic pathways of epithelial kidney cells. Thus, our findings open a new avenue for RCC target therapy
  • Artigo IPEN-doc 27190
    Essential elements as biomarkers of metastatic renal cell carcinoma
    2020 - BELLINI, MARIA H.; SOUZA, ALEXANDRE L. de; SILVA, FABIO F. da; GUILHEN, SABINE N.; FERREIRA, RAFAEL V. de P.; OLIVEIRA, JOAO E. de; MARUMO, JULIO T.
    Renal cell carcinoma (RCC) represents 3% of human malignant tumors and approximately 90% of malignant renal neoplasms. Despite great therapeutic advances in the last decade, metastatic RCC (mRCC) is still considered an incurable disease. In this study, we examined the potential of essential elements as biomarkers of mRCC using an orthotropic metastatic mouse model. Frozen lung and plasma samples from healthy and mRCC-induced mice were lyophilized, digested, and analyzed using inductively coupled plasma mass spectrometry. In metastatic lungs, a significant increase in Ca concentration (268%) was observed, whereas a significant decrease in Cu (23.2%), Fe (17.4%), Mn (38.8%), and Na (11.7%) was observed. The plasma of mRCC-induced mice showed decreased concentrations of Mn (53%), Na (19.7%) and Zn (49,50%) and increased levels of Ca (53%), Cu (39.5%), Our findings revealed marked differences in the concentrations of essential elements in the lung and plasma of the metastatic mouse model. The circulating levels of Ca, Cu, Mn, Na, and Zn could be utilized as diagnostic and therapeutic response biomarkers.