JORGE GABRIEL DOS SANTOS BATISTA

JORGE GABRIEL DOS SANTOS BATISTA

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Copper-Based Nanomaterials for Biologically Relevant Compounds
2024 - BATISTA, JORGE G. dos S.; RODRIGUES, MURILO A.V.; FREITAS, LUCAS F. de; FONSECA, ANA C.M.; RODRIGUES, ADRIANA de S.; CRUZ, CASSIA P.C. da; THIPE, VELAPHI C.; LUGAO, ADEMAR B.
Copper-based nanomaterials have emerged as a new platform in various scientific domains due to their unique physicochemical, biological, and compatibility properties. This review explores the multifaceted applications of copper nanomaterials in the areas of biosensing, drug delivery, cancer therapy, and environmental remediation. The innovative role of copper nanomaterials in biosensors and their exceptional sensitivity and specificity in detecting biomolecules are discussed. Moreover, the potential applications of copper-based nanomaterials in drug delivery systems, highlighting the controlled and targeted release of therapeutic agents’ approaches are explored. In the domain of cancer theragnostic, copper nanomaterials exhibit promising results thus offering practical tools for early diagnosis and advanced treatment modalities. Furthermore, the antimicrobial properties of copper nanoparticles are explored, emphasizing their importance in combating microbial threats and promoting a new era in antimicrobial research. Copper-based nanomaterials could have a significant role in environmental and bioremediation applications. The strong catalytic activity of copper nanoparticles makes them ideal candidates for environmental remediation. In summary, this comprehensive review consolidates the latest advances in copper nanomaterials research, providing valuable information for scientists and researchers involved in diverse fields to promote the development of new applications and technologies.
Desenvolvimento de nanopartículas de ouro (AuNPs) para teranóstica
2021 - RODRIGUES, ADRIANA de S.; LUGAO, ADEMAR B.; BATISTA, JORGE G. dos S.; KUCHINSKI, ADRIANA; FREITAS, LUCAS F. de; CRUZ, CASSIA P.C. da
The state of the art of theranostic nanomaterials for lung, breast, and prostate cancers
2021 - FREITAS, LUCAS F.; FERREIRA, ARYEL H.; THIPE, VELAPHI C.; VARCA, GUSTAVO H.C.; LIMA, CAROLINE S.A.; BATISTA, JORGE G.S.; RIELLO, FABIANE N.; NOGUEIRA, KAMILA; CRUZ, CASSIA P.C.; MENDES, GIOVANNA O.A.; RODRIGUES, ADRIANA S.; SOUSA, THAYNA S.; ALVES, VICTORIA M.; LUGAO, ADEMAR B.
The synthesis and engineering of nanomaterials offer more robust systems for the treatment of cancer, with technologies that combine therapy with imaging diagnostic tools in the so‐called nanotheranostics. Among the most studied systems, there are quantum dots, liposomes, polymeric nanoparticles, inorganic nanoparticles, magnetic nanoparticles, dendrimers, and gold nanoparticles. Most of the advantages of nanomaterials over the classic anticancer therapies come from their optimal size, which prevents the elimination by the kidneys and enhances their permeation in the tumor due to the abnormal blood vessels present in cancer tissues. Furthermore, the drug delivery and the contrast efficiency for imaging are enhanced, especially due to the increased surface area and the selective accumulation in the desired tissues. This property leads to the reduced drug dose necessary to exert the desired effect and for a longer action within the tumor. Finally, they are made so that there is no degradation into toxic byproducts and have a lower immune response triggering. In this article, we intend to review and discuss the state‐of‐the‐art regarding the use of nanomaterials as therapeutic and diagnostic tools for lung, breast, and prostate cancer, as they are among the most prevalent worldwide.
Hybrid gold-protein nanoparticles as radiosensitizers
2020 - FREITAS, L.F.; CRUZ, C.C. da; BATISTA, J.G.; VARCA, G.H.; LUGAO, A.B.; MATHOR, M.B.
Gold nanoparticles present unique optical properties which are dependent upon size and morphology, and consist on a differential interaction with radiation compared to the bulk material. Those nanoparticles can be modified in order to adjust their bioavailability and tissue-targeting, and one of the means to do so is by adsorbing one or more types of proteins onto their surface. Gamma radiation can be helpful in this regard, since it promotes intra- and intermolecular crosslinks in proteins and enables their adsorption onto the metallic nanoparticles’ surfaces. Here we present the results obtained for hybrid gold-protein nanoparticles as radiosensitizers. The nanoparticles were synthesized radiolytically by mixing 5 mmol L-1 NaAuCl4 with 1 mg mL-1 bovine serum albumin (BSA) or papain in the presence of 0.1 mol L-1 tert-butanol and 20% ethanol. The solutions were irradiated with 10 kGy in a multipurpose gamma irradiator (60Co source, 5 kGy per hour) for the radiolytic synthesis of the nanoparticles, and then the resulting red suspension was stored until use. 10^4 cells (MDA-MB-231 line) were seeded in 96-well plates and incubated with a 2:1 mixture of DMEM medium and nanoparticles suspension for 12 hours. Then, the wells were washed with sterile phosphate buffered saline, and fresh DMEM medium was added prior to irradiation in a gamma cell (60Co source, 0.6 kGy per hour) with 10, 30 and 50 Gy. 48 hours later, the cell viability was assessed by MTS assay. The results indicate that the radiation alone slightly stimulated the proliferation of the tumor cells, but this effect was more evident in the presence of gold-papain nanoparticles. The ablative effect due to radiosensitization was observed with 30 and 50 Gy for the cells incubated with gold-BSA nanoparticles, and 10 and 30 Gy for the cells incubated with gold-papain nanoparticles. This difference might be due to a more effective internalization or surface-attachment of nanoparticles when they are coated with papain, and one evidence for this assumption is the fact that the cell culture becomes red after the incubation with gold-papain nanoparticles. Therefore, protein-coated nanoparticles might be effective as radiosensitizers, depending on the coating and dose of radiation.
Green nanotechnology
2020 - BATISTA, J.G.; FREITAS, L.F.; CRUZ, C.C. da; LUGAO, A.B.; RODRIGUES, A.S.; SANTOS, L.O.; PIRES, M.A.
Cancer is among the diseases with the highest mortality rate, with more than 100 different types that occur due to mutations in the genetic material of cells. It is the third leading cause of death worldwide after cardiovascular and infectious diseases. In addition, the number of diseases caused by oxidative stress that results from an imbalance between the formation and neutralization of oxidizing species is increasingly perceived. Oxidative stress is initiated by free radicals and their interactions with biological macromolecules, such as proteins, lipids and DNA, healthy human cells and cause damage to proteins and DNA, with lipid peroxidation. These changes contribute to cancer, atherosclerosis, cardiovascular and inflammatory diseases. All cells are exposed to oxidative stress and therefore oxidation and free radicals play an important role in the development of cancer. Medicinal plants have a special place in cancer management. Several cancer researchers have been carried out studies using traditional medicinal plants, to discover new therapeutic agents that do not have side effects associated with chemotherapeutic agents. Studies using the extract of Annona muricata L., popularly known as araticum, have demonstrated potential anti-inflammatory and anticancer action, due to its antioxidant and immunological properties. The objective of the present work was to develop gold nanoparticles using Annona muricata L. dry extract and verify their physical-chemical characteristics, such as size, shape, and stability. The results obtained show that it is possible to synthesize gold nanoparticles with potential applications in medicine using the extract of araticum. Characterization was performed using the techniques of UV-Vis spectrophotometry, dynamic light scattering (DLS), transmission electron microscopy (TEM). The in vitro stability study of the gold nanoparticles synthesized with the araticum extract was carried out for thirty days. The nanoparticles showed to be stable in different media during this period and the monitoring was done by spectrophotometry.
Stability of gold nanoparticles in different ionic concentrations and pH
2020 - FREITAS, L.F.; CRUZ, C.C. da; BATISTA, J.G.; VARCA, G.H.; LUGAO, A.B.; PIRES, M.A.
There are several protocols for the synthesis of gold nanoparticles, and lately there is a trend for green methods in order to minimize the environmental impacts. The reduction of gold salts by epigallocatechin 3 gallate, for instance, generates stable and uniform nanoparticles without the use of toxic compounds, and so does the radiolytic synthesis protocol. For medical purposes, proteins like albumin and papain are useful coating agents, providing a better biological effectiveness. Here we present a comparison of different synthetic and protein coating protocols for gold nanoparticles regarding their stability in different NaCl concentrations and pH, aiming for the development of nanoparticles that are able to be administered in physiologic solutions to patients. The nanoparticles were synthesized via EGCG (2 mg mL 1) reduction of gold salt (5 mmol L 1) in phosphate buffer pH 7.0. Those nanoparticles were coated or not with albumin or papain (1 mg mL 1) using mercaptopropionic acid. Other protein coated gold nanoparticles were synthesized radiolytically by mixing 5 mmol L 1 NaAuCl4 with 1 mg mL 1 bovine serum albumin (BSA) or papain and 0.1 mol L 1 tert butanol. The solutions were irradiated with 10 kGy (60Co source, 5 kGy h 1) and the resulting suspensions were stored until use. The suspensions were added in 96 well plates to solutions with different pH and NaCl concentrations, and their absorption spectra were taken periodically to verify their stability. It was observed that BSA gold nanoparticles synthesized by both protocols were stable in concentrations of NaCl varying from 0.1% to 14.4% up to 72h. The papain gold nanoparticles synthesized by both protocols were stable in concentrations of NaCl varying from 0.1% to 14.4% up to 48h, but in 72h there was evidence of instability in the lowest and highest NaCl concentrations. The nanoparticles coated just with EGCG (without proteins) were stable in all NaCl concentrations and times, except in the highest concentration after 72h. Regarding the pH, BSA gold nanoparticles and papain gold nanoparticles synthesized radiolytically, as well as EGCG gold nanoparticles were stable at least in pH varying from 5 to 11, in all times analyzed. In conclusion, all the nanoparticles tested are able to be administered to patients in physiological solutions, which have pH around 7.4 and NaCl concentrations around 0.9%, without the risk of aggregation and loss of biological activity.
Nanotecnologia verde
2019 - BATISTA, J.G.S.; LUGAO, A.B.; DA CRUZ, C.C.; DE FREITAS, L.F.; SANTOS, L.O.; RODRIGUES, A.S.
O câncer está entre as doenças com maior índice de mortalidade, com mais de 100 tipos diferentes ocorrendo devido a mutações no material genético das células. É a terceira principal causa de morte em todo o mundo após doenças cardiovasculares e infecciosas. Além disso, percebe-se cada vez mais que muitas das doenças se devem ao “estresse oxidativo” que resulta de um desequilíbrio entre a formação e a neutralização de espécies oxidantes. O estresse oxidativo é iniciado pelos radicais livres, que buscam estabilidade através do pareamento de elétrons com macromoléculas biológicas, como proteínas, lipídios e DNA, em células humanas saudáveis e causam danos às proteínas e ao DNA, juntamente com a peroxidação lipídica. Essas mudanças contribuem para o câncer, aterosclerose, doenças cardiovasculares, envelhecimento e doenças inflamatórias. Todas as células são expostas ao estresse oxidativo e, portanto, a oxidação e os radicais livres desempenham um papel importante no desenvolvimento do câncer e na carcinogênese.Portanto, ainda estão sendo feitos esforços para procurar substâncias eficazes que ocorrem naturalmente e que possam impedir, desacelerar ou reverter o desenvolvimento do câncer. Plantas medicinais têm um lugar especial na gestão do câncer. Diversos estudos de pesquisa sobre o câncer foram realizados utilizando plantas medicinais tradicionais, em um esforço para descobrir novos agentes terapêuticos que não possuem efeitos secundários associados aos presentes agentes quimioterápicos. Estudos utilizando extrato de Annona muricata L., popularmente conhecida como araticum, demonstraram potencial ação anti-inflamatória e anticâncer, devido suas propriedades antioxidante e imunológica. O objetivo do presente trabalho foi sintetizar nanopartículas de ouro utilizando o extrato seco de Annona muricata L. e verificar suas propriedades físico-químicas, como tamanho, formato e estabilidade. E assim unir as propriedades do araticum com as das nanopartículas de ouro que possuem aplicações na terapia e diagnóstico de câncer. Os resultados obtidos, demonstram que é possível sintetizar nanopartículas de ouro com potencial aplicação na medicina utilizando o extrato de araticum. A caracterização foi realizada por meio das técnicas de espectrofotometria UV-Vis, espalhamento de luz dinâmico (DLS), microscopia eletrônica de transmissão (TEM). O estudo de estabilidade in vitro foi realizado misturando-se a solução de nanopartículas de ouro com NaCl, histidina, cisteína, BSA, pH 5, 7 e 9. As nanopartículas apresentaram tamanho médio de 15 nm por TEM e tamanho hidrodinâmico médio de 30 nm por DLS. No teste de estabilidade in vitro, as nanopartículas de ouro sintetizadas com o extrato seco de araticum apresentaram estabilidade em todos os meios testados por até 14 dias, não havendo diminuição na absorbância no comprimento de 525 nm, que é característico do dipolo gerado pela ressonância plasmônica de superfície em nanopartículas de ouro.
Comparison between gold nanoparticles synthesized by radiolysis and by EGCG-driven gold reduction
2020 - FREITAS, LUCAS F. de; CRUZ, CASSIA P.C. da; CAVALCANTE, ADRIANA K.; BATISTA, JORGE G. dos S.; VARCA, GUSTAVO H.C.; MATHOR, MONICA B.; LUGAO, ADEMAR B.
Radiolytic synthesis and phytochemical-driven gold reduction for the generation of nanoparticles are successful examples of Green Chemistry applied for nanomaterials. The present work compares these two green approaches focusing on hydrodynamic size, stability over time, optical properties and toxicity in NIH 3T3 (ATCC® CRL- 1658™) cells and Danio rerio (Zebra Fish). The radiolytic synthesis was performed by mixing 1 mM NaAuCl4; polyvinyl pyrrolidone 0.5%, AgNO3 6×10−5 M, propan-2-ol 0.2 M and acetone 0.06 M, followed by irradiation at 15 kGy (5 kGy h−1, 60Co source). The EGCG-functionalized nanoparticles were synthesized by mixing 1.6 mM of Au with 0.8 mM of EGCG in phosphate buffer (10 mM) for 2 h. Both methods yield the formation of gold nanoparticles featuring plasmon resonance bands at 520–530 nm, polydispersity above 0.3 was relevant only for the radiolytic protocol. Regarding stability over time, after 30 days, the nanoparticles synthesized radiolytically presented no relevant size changes, while some aggregation was observed for the EGCG-particles. The same nanoparticles demonstrated a lack of stability in high ionic strength medium. Slight toxicity was observed for the EGCG-nanoparticles in Danio rerio, with an IC50 calculated as 40.49%, while no IC50 was established within the concentration range of radiolysis-AuNPs used in this study. In conclusion, both green methods generated nanoparticles with good control of size and optical properties, especially via reduction by EGCG. However, the stability and toxicity results were found to be more promising for the radiolytically synthesized gold nanoparticles.