MARIA ELISA CHUERY MARTINS ROSTELATO

Resumo

Graduated in Physics at the Catholic University of São Paulo (1979), master's degree in Nuclear Technology from the University of São Paulo (1997) and PhD in Nuclear Technology from the University of São Paulo (2005). It is currently a senior technologist of the National Nuclear Energy Commission. It has experience in coordinating projects and Nuclear Engineering, with emphasis in Radioisotope Production, acting on the following topics: sources for brachytherapy, Iodine-125 seeds, iridium-192 wires, sources for cancer treatment, radioactive nanosources and dosimetry for brachytherapy. The award for best publication of the year the American Nuclear Society- Latin American Section. The doctoral thesis was awarded honorable mention in the Ministry of Health and UNESCO prize. It acts as reviewer for several journals and as a consultant Ad 'hoc funding agencies. Meets missions International Atomic Energy Agency - IAEA as a consultant. Member of the Science Committee of the International Organization of Medical Physics - IOMP. Vice Coordinator of the Postgraduate Course in Radiation Technology in Health Sciences of the Nuclear and Energy Research Institute. Advisor and professor of the Postgraduate Course in Nuclear Technology of the University of São Paulo. (Text obtained from the Currículo Lattes on October 14th 2021)


Possui graduação em Física pela Pontifícia Universidade Católica de São Paulo (1979), mestrado (1997) e doutorado (2005) em Tecnologia Nuclear pela Universidade de São Paulo. Atualmente é tecnologista senior da Comissão Nacional de Energia Nuclear. Tem experiência na coordenação de projetos e na área de Engenharia Nuclear, com ênfase em Produção de Radioisótopos, atuando principalmente nos seguintes temas: fontes para braquiterapia, sementes de iodo-125, fios de irídio-192, fontes para tratamento de cancer, nanofontes radioativas e dosimetria para braquiterapia. Recebeu o prêmio de melhor publicação do ano da American Nuclear Society- Latin American Section. A tese de doutorado foi distinguida com menção honrosa em prêmio do Ministério da Saúde e UNESCO. Atua como revisora de vários periódicos e como consultora Ad' Hoc de agências de fomento. Atende a missões da International Atomic Energy Agency - IAEA como consultora. Membro do Comitê de Ciência da International Organization of Medical Physics - IOMP. Vice Coordenadora do Curso de Posgraduação de Tecnologia das Radiações em Ciências da Saúde do Instituto de Pesquisas Energéticas e Nucleares. Orientadora e professora do Curso de Posgraduação em Tecnologia Nuclear da Universidade de São Paulo.Coordenadora do Núcleo de Avaliação de Tecnologias em Saúde- IPEN pertencente ao REBRATS- Ministério da Saúde. (Texto extraído do Currículo Lattes em 14 out. 2021)

Projetos de Pesquisa
Unidades Organizacionais
Cargo

Resultados de Busca

Agora exibindo 1 - 10 de 187
  • Artigo IPEN-doc 30371
    Calculation of the budget of uncertainty on measurements size nanoparticles using dynamic light scattering
    2024 - GERALDES, ADRIANA N.; ROSERO, WILMMER A.A.; ROSTELATO, MARIA E.C.M.; SARKIS, JORGE E.S.
    Accurate nanoparticle characterization is essential since it can significantly affect its physicochemical and biological properties. Among physicochemical properties used to characterize nanomaterials, size and size distribution are essential and should be assessed before surveying poisonousness or biocompatibility. Several methods are suitable to evaluate these characteristics including the dynamic light scattering. The aims of the present paper were to propose a methodology to measure nanoparticle size and present the estimation of the particle size uncertainty using the dynamic light scattering technique. The reliability of measurements was ensured by a series of handling precautions and quality criteria for good measurements to be applied for methodology validation using reference material polyvinylpyrrolidone coated silver nanoparticles. The identification and quantification of input quantities to the measurement uncertainties were estimated. The uncertainty concerning the equipment was 1.2% while the repeatability obtained was 1.4%, within the range of values stipulated in the reference standard (less than 5%). The relative standard uncertainties of trueness and repeatability were below the thresholds defined by the International Organization for Standardization. The result of the expanded uncertainty was 3.9% with 95% coverage probability for the reference material.
  • Artigo IPEN-doc 30360
    Review of advances in coating and functionalization of gold nanoparticles
    2024 - ROSERO, WILMMER A.A.; BARBEZAN, ANGELICA B.; SOUZA, CARLA D. de; ROSTELATO, MARIA E.C.M.
    Nanoparticles, especially gold nanoparticles (Au NPs) have gained increasing interest in biomedical applications. Used for disease prevention, diagnosis and therapies, its significant advantages in therapeutic efficacy and safety have been the main target of interest. Its application in immune system prevention, stability in physiological environments and cell membranes, low toxicity and optimal bioperformances are critical to the success of engineered nanomaterials. Its unique optical properties are great attractors. Recently, several physical and chemical methods for coating these NPs have been widely used. Biomolecules such as DNA, RNA, peptides, antibodies, proteins, carbohydrates and biopolymers, among others, have been widely used in coatings of Au NPs for various biomedical applications, thus increasing their biocompatibility while maintaining their biological functions. This review mainly presents a general and representative view of the different types of coatings and Au NP functionalization using various biomolecules, strategies and functionalization mechanisms.
  • Artigo IPEN-doc 30354
    Radioactive gold nanoparticles coated with BSA
    2024 - BARBEZAN, ANGELICA B.; ROSERO, WILMMER A.A.; VIEIRA, DANIEL P.; RIGO, MARIA E.Z.; SILVA, GIOVANA D. da; RODRIGUES, ALEX A.; ALMEIDA, LUIS F. de; SILVA, FABIO F.A. da; RIVERA, ANDY G.; SILVA, NATANAEL G. da; BERNARDES, EMERSON S.; ZEITUNI, CARLOS A.; ROSTELATO, MARIA E.C.M.
    Background: Nanotechnology has revolutionized medicine, especially in oncological treatments. Gold nanoparticles (AuNPs) stand out as an innovative alternative due to their biocompatibility, potential for surface modification, and effectiveness in radiotherapeutic techniques. Given that prostate cancer ranks as one of the leading malignancies among men, there's a pressing need to investigate new therapeutic approaches. Methods: AuNPs coated with bovine serum albumin (BSA) were synthesized and their cytotoxicity was assessed against prostate tumor cell lines (LNCaP and PC-3), healthy prostate cells (RWPE-1), and endothelial control cells (HUVEC) using the MTS/PMS assay. For in vivo studies, BALB/C Nude mice were employed to gauge the therapeutic efficacy, biodistribution, and hematological implications post-treatment with BSA-coated AuNPs. Results: The BSA-coated AuNPs exhibited cytotoxic potential against PC-3 and LNCaP lines, while interactions with RWPE-1 and HUVEC remain subjects for further scrutiny. Within animal models, a diverse therapeutic response was observed, with certain instances indicating complete tumor regression. Biodistribution data emphasized the nanoparticles' affinity towards particular organs, and the majority of hematological indicators aligned with normative standards. Conclusions: BSA-coated AuNPs manifest substantial promise as therapeutic tools in treating prostate cancer. The present research not only accentuates the nanoparticles' efficacy but also stresses the imperative of optimization to ascertain both selectivity and safety. Such findings illuminate a promising trajectory for avant-garde therapeutic modalities, holding substantial implications for public health advancements.
  • Artigo IPEN-doc 29702
    Radioactive seed localization for nonpalpable breast lesions
    2024 - FERREIRA, HORTENCIA H.J.; SOUZA, CARLA D. de; POZZO, LORENA; RIBEIRO, MARTHA S.; ROSTELATO, MARIA E.C.M.
    Background: This study is a systematic review with meta-analysis comparing radioactive seed localization (RSL) versus radio-guided occult lesion localization (ROLL) and wire-guided localization (WGL) for patients with impalpable breast cancer undergoing breast-conserving surgery and evaluating efficacy, safety, and logistical outcomes. The protocol is registered in PROSPERO with the number CRD42022299726. Methods: A search was conducted in the Embase, Lilacs, Pubmed, Scielo, Web of Science, and clinicaltrials.gov databases, in addition to a manual search in the reference list of relevant articles, for randomized clinical trials and cohort studies. Studies selected were submitted to their own data extraction forms and risk of bias analysis according to the ROB 2 and ROBINS 1 tools. A meta-analysis was performed, considering the random effect model, calculating the relative risk or the mean difference for dichotomous or continuous data, respectively. The quality of the evidence generated was analyzed by outcome according to the GRADE tool. Overall, 46 articles met the inclusion criteria and were included in this systematic review; of these, 4 studies compared RSL and ROLL with a population of 1550 women, and 43 compared RSL and WGL with a population of 19,820 women. Results: The results showed that RSL is a superior method to WGL in terms of surgical efficiency in the impalpable breast lesions’ intraoperative localization, and it is at least equivalent to ROLL. Regarding security, RSL obtained results equivalent to the already established technique, the WGL. In addition to presenting promising results, RSL has been proven to be superior to WGL and ROLL technologies.
  • Resumo IPEN-doc 30301
    Gold radioactive nanoparticles for brachytherapy
    2023 - ROSERO, WILMMER A.A.; BARBEZAN, ANGELICA B.; ZEITUNI, CARLOS A.; ROSTELATO, MARIA E.C.M.
    The development of new materials emerges as an alternative to the treatment of cancer, Nanobrachytherapy is born from the union of nanotechnology and brachytherapy being a modality of radiotherapy in which the nanosources are placed close to or in contact with the region to be treated. In nanoparticle synthesis, a key role is played by coatings, certain sizes of uncoated nanoparticles tend to accumulate in tumors due to vessel irregularities. When coated with gum arabic, the size of the nanoparticles is controlled, allowing the particles to properly penetrate the vasculature. The choice of radionuclide depends on the radiobiology of the cancer and the dose deposited in the tissue, which takes into account the type of decay (beta particles penetrate less into the tissue than X-rays and gamma rays), energy and half-life time (which influence the deposited dose per duration of radioisotope). In this work, 198Au will be used, which presents Beta as the main type of emission with an energy of 314.55 KeV, gamma 411.8 KeV, and a half-life of 2.7 days. The synthesis of radioactive nanoparticles is carried out in a closed chemical reactor, developed during the tests. The chemical reactor gold nanoparticles and precursor chloroauric acid are obtained. The morphology of nanoparticles and their size is guaranteed cold by TEM and radioactive by DLS. With an average diameter (TEM) of 5 nm, gum arabic nanoparticles have a curious property, when solution they form stable agglomerates of 45 nm (DLS), optimal size for in vitro and in vivo tests. They were tested with cancer cells of prostate and mice. In one of the evaluated LNCaP strains, 11% cytotoxicity was observed at the lowest concentration (0.9 μCi/well). At the concentration of 1.8 μCi/well, there was cell proliferation and at the highest concentration (2.7 μCi/well) it showed 32% cytotoxicity. After statistical analysis, the results revealed that although none of the animals showed regression of tumor mass. Tumor growth was slower at treated animals when compared to the tumor growth of the control animals (zero dose). Signaling us then that there is a positive effect on therapeutic efficacy, but these data suggest that the activity used was relatively low to achieve the regression of this mass. In the next experiments we will increase the injected radioactive activity to confirm these results and evaluate its real therapeutic potential.
  • Resumo IPEN-doc 30158
    Development of radioactive nanoparticles functionalized with gum arabic to be used in nanobrachytherapy
    2023 - ROSERO, WILMMER A.A.; BARBEZAN, ANGELICA B.; RIGO, MARIA E.Z.; RODRIGUES, PRISCILA S.; TEODORO, LARA E.H.; TALACIMON, CRISTHIAN F.; ZEITUNI, CARLOS A.; ROSTELATO, MARIA E.C.M.
    The development of new materials emerges as an alternative to the treatment of cancer, from the combination of nanotechnology and brachytherapy a new area of research was born, Nanobrachytherapy, which through the properties of nanometric materials can achieve better results in the fight against cancer. The objective of this work is classified as radiotherapy, which consists of the use of ionizing radiation to destroy or inhibit the growth of abnormal cells that form a tumor. [1] The ability to integrate NPsAu into biological systems is due to the nanometric dimensions of NPsAu probes which facilitate their incorporation into biological systems, as well as their bioconjugation and non-cytotoxic potential. [2] Taking into account the previous objective, gold was selected as the base element for obtaining nanometric systems, which due to its chemical richness and especially due to the intrinsic properties of one of its radioisotopes, which would allow us in theory to meet the stated objective. . Initially, it was based on the knowledge of the literature, and non-radioactive nanometric systems were obtained, and after a series of stability, characterization and application tests, the radioactive nanometric systems were obtained. Working with radioactive systems posed a great challenge, and up to now it presents us with situations to solve, but we have managed to create a methodology for obtaining, characterizing and applying radioactive gold nanoparticles, and also obtaining positive results from their application.
  • Resumo IPEN-doc 30157
    Possible molds for a new brachytherapy source for spinal and intracranial cancer
    2023 - TEODORO, LARA E.H.; TALACIMON, CRISTHIAN F.; RIGO, MARIA E.Z.; RODRIGUES, PRISCILA S.; NOGUEIRA, THUANY C.; ROSERO, WILMMER A.A.; VIEIRA, JOSE M.; ZEITUNI, CARLOS A.; ROSTELATO, MARIA E.C.M.
    Central Nervous System (CNS) cancer is the 18th most incident cancer in the world, according to the World Health Organization (WHO), and in 2020, in Brazil, it represented 2.5% of all new cancer deaths [1]. Brachytherapy is a type of radiotherapy that positions the radioactive source close to (or in contact with) the tumor. Thus, the targeted region receives most of the dose, protecting the healthy tissues adjacent to the tumor. A promising radioactive source for use in brachytherapy for the treatment of CNS cancer is phosphorus-32 in a polymeric substrate. This source has been prominent as a minimally invasive treatment for craniopharyngiomas and in the treatment of metastatic bone diseases in general [2]. To produce such a source, it is necessary to search for possible molds that can conform the polymeric material into the desired format. Several tests were carried out to determine the best mold for the radioactive source manufacture. Different materials were tested to observe if the mold material would react with the source's components, silicone rubber mixed with orthophosphoric acid. In order to be considered a possible mold, the mixture should cure; if the cure did not concretize, the mold would be descarted. It was concluded that polystyrene (PS) obtained the best result, due to the ease of unmold after the resin curing process.
  • Resumo IPEN-doc 30149
    Thermoluminescent dosimetry planning through MNCP
    2023 - NOGUEIRA, THUANY; TALACIMON, CRISTHIAN; TEODORO, LARA; RIGO, MARIA; RODRIGUES, PRISCILA; ANGELOCCI, LUCAS; NOVAES, HAMONA; ZEITUNI, CARLOS; ROSTELATO, MARIA
  • Resumo IPEN-doc 30146
    Nationalization of brachytherapy radioactive sources in Brazil and the importance of IAEA cooperation
    2023 - ROSTELATO, MARIA E.C.M.; FEHER, ANSELMO; ZEITUNI, CARLOS A.; ROSERO, WILMMER A.A.; SOUZA, CARLA D. de; MOURA, JOÃO A.
    Brazil has a cancer incidence of about 625,000 cases a year. It is a public health problem, demanding constant efforts to deliver for patients the most efficient treatment modalities, improving their life expectancy and quality. Brachytherapy is a type of Radiotherapy where the radioactive source is placed close to or inside the tumor. The main advantage of the technique is to deliver the maximum dose in the target, saving healthy tissues. In Brazil, Our group had the objective of producing sources nationally, diminishing treatment costs, enabling the treatment to more patients. Some of our projects are developed in collaboration with the International Atomic Energy Agency-IAEA by technical cooperation projects. The IAEA participation is very important to provide technological transfer through scientific visits, expert missions, and contacts with more advanced centers. The financial support is also important, allowing us to buy the necessary equipment to make these cancer treatment sources production feasible in Brazil. Our team has received training through fellowships. We received some experts and organized several workshops to propagate the Brachytherapy technique at national and Latin American level. For producing new sources, five major areas must be considered: 1) source production: nuclear activation and/or radiochemical reaction; 2) welding; 3) quality control: leakage tests; 4) dosimetry and metrology; 5) operational procedures; 6) validation studies. To perform all steps, a multidisciplinary team works together to overcome difficulties. Our major projects are: Iridium-192 pellets: In Brazil there are 150 afterloading machines with pellets that replacement every 4 months (about 450 Iridium-192 sources a year). Our new production line, with the support of IAEA, is in progress, with the hot-cell being installed in a brand-new facility. Iridium-192 wires: In production since 1997, also supported by IAEA. The wire is activated at IPEN’s IEA-R1 reactor for 30 hours with 5x1013 n/cm-2.s-1 neutron flux resulting in 7.1 GBq (192 mCi) maximum activity. Iridium-192 seed: New seed for ophthalmic cancer treatment. The core presented 90% activity homogeneity. We are making the experimental dosimetry and Monte Carlo simulation. Iodine-125 seeds: Largely used in low dose brachytherapy. I-125 binding yield achieved with our new reaction was 90%; Laser welding presented 70% efficiency. Approved in all leakage tests. Our Iodine-125 seeds laboratory production is 90% ready. Other ongoing projects: polymeric Phosphorus-32 source for spinal cancer treatment, Gold-198 nanoparticles for prostate, breast, and liver cancer treatment, Iodine-125 seed as markers for non-palpable cancers, and dosimetry calculations for all new sources. All the projects are advancing, despite national funding difficulties. Withing those, several mSc, Phd, and Post-doc are getting their degrees. We will continue to develop new products hoping to help the Brazilian population fight against cancer. The support of IAEA has proven to be of the utmost importance for these projects not only in direct funding, but in providing knowledge to our team, the possibility to share information with the scientific community, and to form the next generation of scientists.
  • Resumo IPEN-doc 30145
    Synthesis, activation and application testing of gold nanoparticles for nanobrachytherapy
    2023 - ROSERO, WILMMER A.A.; BARBEZAN, ANGELICA B.; ROSTELATO, MARIA E.C.M.; SOUZA, CARLA D. de; NOGUEIRA, BEATRIZ R.; ZEITUNI, CARLOS A.
    For more than 50 years, the Energy and Nuclear Research Institute IPEN, has been offering solutions to Brazil through nuclear technology. Thus, one of the main areas where IPEN has contributed assertively is medicine. Reaching the level of 32 radiopharmaceuticals and radioactive sources intended both for therapy and for the diagnosis of several pathologies, including cancer, which are obtained with the help of the two nuclear reactors and two cyclotrons present in the institution. The Institute has a team for the development, production and distribution of radioactive sources for brachytherapy, such as 192 Ir wires and 125 I seeds. Brachytherapy is a cancer treatment technique where the radioactive source is placed close to or in contact with the lesion. The great advantage of the technique is to save healthy tissues. Currently, we are working on obtaining nanometric materials that can be applied in the emerging nano brachytherapy, because of its properties and characteristics at the nanometric level, gold has been the subject of studies and tests. Elemental Au gold can be activated 198 Au inside a nuclear reactor, and has β- decay and a half-life of 2.7 days, which makes it ideal for short-term irradiations. In addition, gold in the form of nanoparticles has a completely different chemistry, with gold nanoparticles (AuNPs) being easily functionalized by a large part of molecular and polymeric binders, which may present favorable characteristics for the studies, and together with AuNPs they are able to work synergistically to achieve greater efficiencies. Currently, AuNPs have been successfully functionalized with gum arabic (GA), a coating widely used in the cosmetic and food industry, which is low cost and along with nanoparticles has shown biocompatibility with different cell groups and has been shown to be very stable over time. The project includes studies regarding the synthesis of nanoparticles, coating, cytotoxicity of AuNPs in vitro "cold" (non-radioactive) and the development of activation protocols in the nuclear reactor. In the next phase, after activation, in the reactor, "hot" tests will be performed in vitro and in vivo.