SOLANGE KAZUMI SAKATA

SOLANGE KAZUMI SAKATA

(Fonte: Lattes)

Resumo

Possui graduação em Química bacharelado e licenciatura pela Universidade de São Paulo. Doutorado na área de Química Orgânica, com ênfase em Eletrossintese Orgânica pelo Instituto de Química da Universidade de São Paulo. Pós - doutorados em Biotecnologia no Scripps Institution of Oceanography na University of California - San Diego -USA) e no Instituto de Química da Universidade de São Paulo. Foi pesquisadora visitante no Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB-Stuttgart - Alemanha no estudo do metagenoma na produção de enzimas para fins catalíticos e no Centro Tecnológico da Marinha de São Paulo (CTM-SP) no desenvolvimento e caracterização de polímeros. Atualmente é pesquisadora do Instituto de Pesquisas Energéticas e Nucleares (IPEN- SP / CNEN) no Centro de Tecnologia das Radiações e estuda o efeitos das radiações em nano materiais de carbono. (Texto extraído do Currículo Lattes em 27 dez. 2021).

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Aptasensing of beta-amyloid (Aβ(1− 42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendrites
2023 - NEGAHDARY, MASOUD; VELOSO, WILLIAM B.; BACIL, RAPHAEL P.; BUORO, RAFAEL M.; GUTZ, IVANO G.R.; PAIXAO, THIAGO R.L.C.; LAGO, CLAUDIMIR L. do; SAKATA, SOLANGE K.; MELONI, GABRIEL N.; FRANCA, MESAQUE C.; OLIVEIRA, THAWAN G. de; AMEKU, WILSON A.; DURAZZO, MICHELANGELO; ANGNES, LUCIO
In this study, beta-amyloid (Aβ(1− 42)), an essential biomarker for diagnosing Alzheimer’s disease (AD), was detected via an electrochemical aptasensing platform. Here, an innovative signal transducer was developed using a CO2 laser-ablated 3D-printed electrode modified with leaf-shaped gold nanodendrites (LSG NDs, mean diameter: ~ 92 nm), which could provide an efficient interface for immobilizing aptamer strands. The modified electrode with LSG NDs exhibited an enhancement in its electrochemically active surface area about 7 times, compared with the bare electrode. This modification showed that the size, morphology, and distributions of LSG NDs in amplifying electrochemical signals might effectively provide a highly sensitive infrastructure for analyte detection. The strands of a thiol-functionalized aptamer sequence interacted with the gold surface, which created an optimized biointerface to detect Aβ(1− 42) in a linear range from 0.1 pg mL− 1 to 10 ng mL− 1 (limit of detection (LOD): 84 fg mL− 1 , (S/N = 3)). The developed aptasensor confirmed satisfactory stability, desired reproducibility and regeneration, and minimal impact of interfering agents. In addition, the application of this aptasensor was monitored via an assay of spiked analyte concentrations in 20 samples, including cerebrospinal fluid (CSF) and human serum.
Disposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samples
2021 - BALDO, THAISA A.; PROENCA, CAMILA dos A.; FELIX, FABIANA da S.; FREITAS, TAYANE A.; SAKATA, SOLANGE K.; ANGNES, LUCIO; FARIA, RONALDO C.
Food allergies have been increasing all over the world. Egg is an important component in the food industries and the second most common cause of food allergy, shortly after milk. In the wine industry, egg white is applied as a fining agent for tannin removal. In this study, a sandwich-based immunoassay for ultrasensitive detection of ovalbumin (OVA) in wine samples was developed. The assay involves the use of magnetic beads (MBs) decorated with a polyclonal anti-OVA antibody (Ab2) and horseradish peroxidase (HRP), used as label for the quantification in a disposable electrochemical microfluidic device (DEμD) here developed. The Ab2-MB-HRP prepared was applied to capture, separate, and pre-concentrate OVA from wine samples. In the DEμD, OVA was immune-magnetically captured (OVA-Ab2-MB-HRP), producing a sandwich structure (GO-Ab1-OVA-Ab2-MB-HRP) on the electrode's surface. This arrangement results in an ultrasensitive device, achieving the ultralow limit of detection of 0.2 fg mL−1 OVA. Five samples of wines were analyzed by using the immuno-magneto-assay which presents excellent accuracy compared with enzyme-linked immunosorbent assay (ELISA).