Aptasensing of beta-amyloid (Aβ(1− 42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendrites
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2023
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Sensors and Actuators B: Chemical
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In this study, beta-amyloid (Aβ(1− 42)), an essential biomarker for diagnosing Alzheimer’s disease (AD), was
detected via an electrochemical aptasensing platform. Here, an innovative signal transducer was developed using
a CO2 laser-ablated 3D-printed electrode modified with leaf-shaped gold nanodendrites (LSG NDs, mean diameter: ~ 92 nm), which could provide an efficient interface for immobilizing aptamer strands. The modified
electrode with LSG NDs exhibited an enhancement in its electrochemically active surface area about 7 times,
compared with the bare electrode. This modification showed that the size, morphology, and distributions of LSG
NDs in amplifying electrochemical signals might effectively provide a highly sensitive infrastructure for analyte
detection. The strands of a thiol-functionalized aptamer sequence interacted with the gold surface, which created
an optimized biointerface to detect Aβ(1− 42) in a linear range from 0.1 pg mL− 1 to 10 ng mL− 1 (limit of detection
(LOD): 84 fg mL− 1
, (S/N = 3)). The developed aptasensor confirmed satisfactory stability, desired reproducibility
and regeneration, and minimal impact of interfering agents. In addition, the application of this aptasensor was
monitored via an assay of spiked analyte concentrations in 20 samples, including cerebrospinal fluid (CSF) and
human serum.
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NEGAHDARY, MASOUD; VELOSO, WILLIAM B.; BACIL, RAPHAEL P.; BUORO, RAFAEL M.; GUTZ, IVANO G.R.; PAIXAO, THIAGO R.L.C.; LAGO, CLAUDIMIR L. do; SAKATA, SOLANGE K.; MELONI, GABRIEL N.; FRANCA, MESAQUE C.; OLIVEIRA, THAWAN G. de; AMEKU, WILSON A.; DURAZZO, MICHELANGELO; ANGNES, LUCIO. Aptasensing of beta-amyloid (Aβ(1− 42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendrites. Sensors and Actuators B: Chemical, v. 393, p. 1-11, 2023. DOI: 10.1016/j.snb.2023.134130. Disponível em: http://repositorio.ipen.br/handle/123456789/34385. Acesso em: 15 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.