Aptasensing of beta-amyloid (Aβ(1− 42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendrites
dc.contributor.author | NEGAHDARY, MASOUD | pt_BR |
dc.contributor.author | VELOSO, WILLIAM B. | pt_BR |
dc.contributor.author | BACIL, RAPHAEL P. | pt_BR |
dc.contributor.author | BUORO, RAFAEL M. | pt_BR |
dc.contributor.author | GUTZ, IVANO G.R. | pt_BR |
dc.contributor.author | PAIXAO, THIAGO R.L.C. | pt_BR |
dc.contributor.author | LAGO, CLAUDIMIR L. do | pt_BR |
dc.contributor.author | SAKATA, SOLANGE K. | pt_BR |
dc.contributor.author | MELONI, GABRIEL N. | pt_BR |
dc.contributor.author | FRANCA, MESAQUE C. | pt_BR |
dc.contributor.author | OLIVEIRA, THAWAN G. de | pt_BR |
dc.contributor.author | AMEKU, WILSON A. | pt_BR |
dc.contributor.author | DURAZZO, MICHELANGELO | pt_BR |
dc.contributor.author | ANGNES, LUCIO | pt_BR |
dc.coverage | Internacional | |
dc.date.accessioned | 2024-01-29T13:01:09Z | |
dc.date.available | 2024-01-29T13:01:09Z | |
dc.date.issued | 2023 | pt_BR |
dc.description.abstract | In this study, beta-amyloid (Aβ(1− 42)), an essential biomarker for diagnosing Alzheimer’s disease (AD), was detected via an electrochemical aptasensing platform. Here, an innovative signal transducer was developed using a CO2 laser-ablated 3D-printed electrode modified with leaf-shaped gold nanodendrites (LSG NDs, mean diameter: ~ 92 nm), which could provide an efficient interface for immobilizing aptamer strands. The modified electrode with LSG NDs exhibited an enhancement in its electrochemically active surface area about 7 times, compared with the bare electrode. This modification showed that the size, morphology, and distributions of LSG NDs in amplifying electrochemical signals might effectively provide a highly sensitive infrastructure for analyte detection. The strands of a thiol-functionalized aptamer sequence interacted with the gold surface, which created an optimized biointerface to detect Aβ(1− 42) in a linear range from 0.1 pg mL− 1 to 10 ng mL− 1 (limit of detection (LOD): 84 fg mL− 1 , (S/N = 3)). The developed aptasensor confirmed satisfactory stability, desired reproducibility and regeneration, and minimal impact of interfering agents. In addition, the application of this aptasensor was monitored via an assay of spiked analyte concentrations in 20 samples, including cerebrospinal fluid (CSF) and human serum. | pt_BR |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | pt_BR |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | pt_BR |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipID | FAPESP: 019/27021–4; 18/08782–1; 17/13137–5; 14/50867–3 | pt_BR |
dc.description.sponsorshipID | CNPq: 311847–2018-8; 302839/2020–8; 465389/2014–7 | pt_BR |
dc.description.sponsorshipID | CAPES: 001 | |
dc.format.extent | 1-11 | pt_BR |
dc.identifier.citation | NEGAHDARY, MASOUD; VELOSO, WILLIAM B.; BACIL, RAPHAEL P.; BUORO, RAFAEL M.; GUTZ, IVANO G.R.; PAIXAO, THIAGO R.L.C.; LAGO, CLAUDIMIR L. do; SAKATA, SOLANGE K.; MELONI, GABRIEL N.; FRANCA, MESAQUE C.; OLIVEIRA, THAWAN G. de; AMEKU, WILSON A.; DURAZZO, MICHELANGELO; ANGNES, LUCIO. Aptasensing of beta-amyloid (Aβ(1− 42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendrites. <b>Sensors and Actuators B: Chemical</b>, v. 393, p. 1-11, 2023. DOI: <a href="https://dx.doi.org/10.1016/j.snb.2023.134130">10.1016/j.snb.2023.134130</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/34385. | |
dc.identifier.doi | 10.1016/j.snb.2023.134130 | pt_BR |
dc.identifier.issn | 0925-4005 | |
dc.identifier.orcid | 0000-0002-2147-8295 | pt_BR |
dc.identifier.orcid | 0000-0001-6072-5853 | pt_BR |
dc.identifier.orcid | https://orcid.org/0000-0002-2147-8295 | |
dc.identifier.orcid | https://orcid.org/0000-0001-6072-5853 | |
dc.identifier.percentilfi | 92.8 | |
dc.identifier.percentilfiCiteScore | 94.30 | |
dc.identifier.uri | http://repositorio.ipen.br/handle/123456789/34385 | |
dc.identifier.vol | 393 | pt_BR |
dc.relation.ispartof | Sensors and Actuators B: Chemical | |
dc.rights | openAccess | pt_BR |
dc.subject | electrochemistry | |
dc.subject | biological markers | |
dc.subject | lasers | |
dc.subject | pyrolysis | |
dc.subject | electrodes | |
dc.subject | 3d printing | |
dc.subject | gold | |
dc.subject | dendrites | |
dc.subject | nervous system diseases | |
dc.title | Aptasensing of beta-amyloid (Aβ(1− 42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendrites | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dspace.entity.type | Publication | |
ipen.autor | MICHELANGELO DURAZZO | |
ipen.autor | SOLANGE KAZUMI SAKATA | |
ipen.codigoautor | 90 | |
ipen.codigoautor | 7038 | |
ipen.contributor.ipenauthor | MICHELANGELO DURAZZO | |
ipen.contributor.ipenauthor | SOLANGE KAZUMI SAKATA | |
ipen.date.recebimento | 24-01 | |
ipen.identifier.fi | 8.0 | |
ipen.identifier.fiCiteScore | 14.6 | |
ipen.identifier.ipendoc | 29946 | |
ipen.identifier.iwos | WoS | pt_BR |
ipen.range.fi | 6.000 ou mais | |
ipen.range.percentilfi | 75.00 - 100.00 | |
ipen.type.genre | Artigo | |
relation.isAuthorOfPublication | 6596a057-886c-4d04-b5eb-ac1a38eb82a9 | |
relation.isAuthorOfPublication | fb62f288-104d-4352-aed0-a18e0da21a1b | |
relation.isAuthorOfPublication.latestForDiscovery | fb62f288-104d-4352-aed0-a18e0da21a1b | |
sigepi.autor.atividade | DURAZZO, MICHELANGELO:90:410:N | pt_BR |
sigepi.autor.atividade | SAKATA, SOLANGE K.:7038:220:N | pt_BR |