Aptasensing of beta-amyloid (Aβ(1− 42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendrites

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dc.contributor.authorNEGAHDARY, MASOUDpt_BR
dc.contributor.authorVELOSO, WILLIAM B.pt_BR
dc.contributor.authorBACIL, RAPHAEL P.pt_BR
dc.contributor.authorBUORO, RAFAEL M.pt_BR
dc.contributor.authorGUTZ, IVANO G.R.pt_BR
dc.contributor.authorPAIXAO, THIAGO R.L.C.pt_BR
dc.contributor.authorLAGO, CLAUDIMIR L. dopt_BR
dc.contributor.authorSAKATA, SOLANGE K.pt_BR
dc.contributor.authorMELONI, GABRIEL N.pt_BR
dc.contributor.authorFRANCA, MESAQUE C.pt_BR
dc.contributor.authorOLIVEIRA, THAWAN G. dept_BR
dc.contributor.authorAMEKU, WILSON A.pt_BR
dc.contributor.authorDURAZZO, MICHELANGELOpt_BR
dc.contributor.authorANGNES, LUCIOpt_BR
dc.coverageInternacional
dc.date.accessioned2024-01-29T13:01:09Z
dc.date.available2024-01-29T13:01:09Z
dc.date.issued2023pt_BR
dc.description.abstractIn this study, beta-amyloid (Aβ(1− 42)), an essential biomarker for diagnosing Alzheimer’s disease (AD), was detected via an electrochemical aptasensing platform. Here, an innovative signal transducer was developed using a CO2 laser-ablated 3D-printed electrode modified with leaf-shaped gold nanodendrites (LSG NDs, mean diameter: ~ 92 nm), which could provide an efficient interface for immobilizing aptamer strands. The modified electrode with LSG NDs exhibited an enhancement in its electrochemically active surface area about 7 times, compared with the bare electrode. This modification showed that the size, morphology, and distributions of LSG NDs in amplifying electrochemical signals might effectively provide a highly sensitive infrastructure for analyte detection. The strands of a thiol-functionalized aptamer sequence interacted with the gold surface, which created an optimized biointerface to detect Aβ(1− 42) in a linear range from 0.1 pg mL− 1 to 10 ng mL− 1 (limit of detection (LOD): 84 fg mL− 1 , (S/N = 3)). The developed aptasensor confirmed satisfactory stability, desired reproducibility and regeneration, and minimal impact of interfering agents. In addition, the application of this aptasensor was monitored via an assay of spiked analyte concentrations in 20 samples, including cerebrospinal fluid (CSF) and human serum.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 019/27021–4; 18/08782–1; 17/13137–5; 14/50867–3pt_BR
dc.description.sponsorshipIDCNPq: 311847–2018-8; 302839/2020–8; 465389/2014–7pt_BR
dc.description.sponsorshipIDCAPES: 001
dc.format.extent1-11pt_BR
dc.identifier.citationNEGAHDARY, MASOUD; VELOSO, WILLIAM B.; BACIL, RAPHAEL P.; BUORO, RAFAEL M.; GUTZ, IVANO G.R.; PAIXAO, THIAGO R.L.C.; LAGO, CLAUDIMIR L. do; SAKATA, SOLANGE K.; MELONI, GABRIEL N.; FRANCA, MESAQUE C.; OLIVEIRA, THAWAN G. de; AMEKU, WILSON A.; DURAZZO, MICHELANGELO; ANGNES, LUCIO. Aptasensing of beta-amyloid (Aβ(1− 42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendrites. <b>Sensors and Actuators B: Chemical</b>, v. 393, p. 1-11, 2023. DOI: <a href="https://dx.doi.org/10.1016/j.snb.2023.134130">10.1016/j.snb.2023.134130</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/34385.
dc.identifier.doi10.1016/j.snb.2023.134130pt_BR
dc.identifier.issn0925-4005
dc.identifier.orcid0000-0002-2147-8295pt_BR
dc.identifier.orcid0000-0001-6072-5853pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2147-8295
dc.identifier.orcidhttps://orcid.org/0000-0001-6072-5853
dc.identifier.percentilfi92.8
dc.identifier.percentilfiCiteScore94.30
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/34385
dc.identifier.vol393pt_BR
dc.relation.ispartofSensors and Actuators B: Chemical
dc.rightsopenAccesspt_BR
dc.subjectelectrochemistry
dc.subjectbiological markers
dc.subjectlasers
dc.subjectpyrolysis
dc.subjectelectrodes
dc.subject3d printing
dc.subjectgold
dc.subjectdendrites
dc.subjectnervous system diseases
dc.titleAptasensing of beta-amyloid (Aβ(1− 42)) by a 3D-printed platform integrated with leaf-shaped gold nanodendritespt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorMICHELANGELO DURAZZO
ipen.autorSOLANGE KAZUMI SAKATA
ipen.codigoautor90
ipen.codigoautor7038
ipen.contributor.ipenauthorMICHELANGELO DURAZZO
ipen.contributor.ipenauthorSOLANGE KAZUMI SAKATA
ipen.date.recebimento24-01
ipen.identifier.fi8.0
ipen.identifier.fiCiteScore14.6
ipen.identifier.ipendoc29946
ipen.identifier.iwosWoSpt_BR
ipen.range.fi6.000 ou mais
ipen.range.percentilfi75.00 - 100.00
ipen.type.genreArtigo
relation.isAuthorOfPublication6596a057-886c-4d04-b5eb-ac1a38eb82a9
relation.isAuthorOfPublicationfb62f288-104d-4352-aed0-a18e0da21a1b
relation.isAuthorOfPublication.latestForDiscoveryfb62f288-104d-4352-aed0-a18e0da21a1b
sigepi.autor.atividadeDURAZZO, MICHELANGELO:90:410:Npt_BR
sigepi.autor.atividadeSAKATA, SOLANGE K.:7038:220:Npt_BR
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