Efficiency of NaOCl and laser-assisted photosensitization on the reduction of Enterococcus faecalis in vitro
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Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics
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Objective: To investigate the action of a red laser associated with a photosensitizer on the reduction of Enterococcus faecalis in dental root canal in vitro. Study design: Thirty prepared teeth with single canals were contaminated. The chemical group was irrigated with 0.5% NaOCl and left flooded for 30 minutes. In the laser group, a paste-based photosensitizer was maintained in the root canals for 5 minutes, and then irradiated with a laser at 685 nm using an optical fiber for an E of 1.8 J during 3 minutes. After treatment, the canal content was collected, serially diluted, and cultured to determine the number of colony-forming units. Results: Photosensitizer alone or laser alone did not have any bactericidal effect. Chemical solution reduced viable bacteria in 93.25%. Laser photosensitization resulted in a reduction of 99.2%, a significantly higher bacterial reduction than NaOCl. Conclusion: Laser photosensitization was effective for reducing E. faecalis in root canals and could be an adjunct to endodontic treatment.
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GARCEZ, AGUINALDO S.; NUNEZ, SILVIA C.; LAGE MARQUES, JOSE L.; JORGE, ANTONIO O.C.; RIBEIRO, MARTHA S. Efficiency of NaOCl and laser-assisted photosensitization on the reduction of Enterococcus faecalis in vitro. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics, v. 102, p. e93-e98, 2006. DOI: 10.1016/j.tripleo.2006.02.015. Disponível em: http://repositorio.ipen.br/handle/123456789/5346. Acesso em: 30 Dec 2025.
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