Effect of virulence factors on the photodynamic inactivation of Cryptococcus neoformans

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dc.contributor.authorPRATES, RENATO A.pt_BR
dc.contributor.authorFUCHS, BETH B.pt_BR
dc.contributor.authorMIZUMO, KAZUEpt_BR
dc.contributor.authorNAQVI, QURATpt_BR
dc.contributor.authorKATO, ILKA T.pt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.contributor.authorMYLONAKIS, ELEFTHERIOSpt_BR
dc.contributor.authorTEGOS, GEORGE P.pt_BR
dc.contributor.authorHAMBLIN, MICHAEL R.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2014-07-31T11:35:53Zpt_BR
dc.date.accessioned2014-07-31T11:53:05Z
dc.date.available2014-07-31T11:35:53Zpt_BR
dc.date.available2014-07-31T11:53:05Z
dc.date.issued2013pt_BR
dc.description.abstractOpportunistic fungal pathogens may cause an array of superficial infections or serious invasive infections, especially in immunocompromised patients. Cryptococcus neoformans is a pathogen causing cryptococcosis in HIV/AIDS patients, but treatment is limited due to the relative lack of potent antifungal agents. Photodynamic inactivation (PDI) uses the combination of non-toxic dyes called photosensitizers and harmless visible light, which produces singlet oxygen and other reactive oxygen species that produce cell inactivation and death. We report the use of five structurally unrelated photosensitizers (methylene blue, Rose Bengal, selenium derivative of a Nile blue dye, a cationic fullerene and a conjugate between poly-L-lysine and chlorin(e6)) combined with appropriate wavelengths of light to inactivate C. neoformans. Mutants lacking capsule and laccase, and culture conditions that favoured melanin production were used to probe the mechanisms of PDI and the effect of virulence factors. The presence of cell wall, laccase and melanin tended to protect against PDI, but the choice of the appropriate photosensitizers and dosimetry was able to overcome this resistance.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP:10/13313-9pt_BR
dc.format.extente54387pt_BR
dc.identifier.citationPRATES, RENATO A.; FUCHS, BETH B.; MIZUMO, KAZUE; NAQVI, QURAT; KATO, ILKA T.; RIBEIRO, MARTHA S.; MYLONAKIS, ELEFTHERIOS; TEGOS, GEORGE P.; HAMBLIN, MICHAEL R. Effect of virulence factors on the photodynamic inactivation of Cryptococcus neoformans. <b>PLoS ONE</b>, v. 8, n. 1, p. e54387, 2013. DOI: <a href="https://dx.doi.org/10.1371/journal.pone.0054387">10.1371/journal.pone.0054387</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/8448.
dc.identifier.doi10.1371/journal.pone.0054387
dc.identifier.fasciculo1pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/8448pt_BR
dc.identifier.vol8pt_BR
dc.relation.ispartofPLoS ONEpt_BR
dc.rightsopenAccessen
dc.subjectfungipt_BR
dc.subjectfungal diseasespt_BR
dc.subjecttherapypt_BR
dc.subjectinactivationpt_BR
dc.subjectphotosensitivitypt_BR
dc.subjectantimicrobial agentspt_BR
dc.subjectmethylene bluept_BR
dc.subjectrose bengalpt_BR
dc.subjectyeastspt_BR
dc.subjectscanning electron microscopypt_BR
dc.titleEffect of virulence factors on the photodynamic inactivation of Cryptococcus neoformanspt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorMICHAEL R HAMBLIN
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorILKA TIEMY KATO
ipen.autorRENATO ARAÚJO PRATES
ipen.codigoautor6893
ipen.codigoautor574
ipen.codigoautor3089
ipen.codigoautor4028
ipen.contributor.ipenauthorMICHAEL R HAMBLIN
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorILKA TIEMY KATO
ipen.contributor.ipenauthorRENATO ARAÚJO PRATES
ipen.date.recebimento13-10pt_BR
ipen.identifier.fi3.534pt_BR
ipen.identifier.ipendoc19115pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi3.000 - 4.499
ipen.type.genreArtigo
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sigepi.autor.atividadePRATES, RENATO A.:4028:920:Spt_BR
sigepi.autor.atividadept_BR
sigepi.autor.atividadeFUCHS, BETH B.:-1:-1:Npt_BR
sigepi.autor.atividadept_BR
sigepi.autor.atividadeMIZUMO, KAZUE:-1:-1:Npt_BR
sigepi.autor.atividadeNAQVI, QURAT:-1:-1:Npt_BR
sigepi.autor.atividadept_BR
sigepi.autor.atividadeKATO, ILKA T.:3089:920:Npt_BR
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
sigepi.autor.atividadeMYLONAKIS, ELEFTHERIOS:-1:-1:Npt_BR
sigepi.autor.atividadept_BR
sigepi.autor.atividadept_BR
sigepi.autor.atividadeTEGOS, GEORGE P.:-1:-1:Npt_BR
sigepi.autor.atividadeHAMBLIN, MICHAEL R.:6893:-1:Npt_BR
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