Effect of virulence factors on the photodynamic inactivation of Cryptococcus neoformans
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2013
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Opportunistic fungal pathogens may cause an array of superficial infections or serious invasive infections, especially in
immunocompromised patients. Cryptococcus neoformans is a pathogen causing cryptococcosis in HIV/AIDS patients, but
treatment is limited due to the relative lack of potent antifungal agents. Photodynamic inactivation (PDI) uses the
combination of non-toxic dyes called photosensitizers and harmless visible light, which produces singlet oxygen and other
reactive oxygen species that produce cell inactivation and death. We report the use of five structurally unrelated
photosensitizers (methylene blue, Rose Bengal, selenium derivative of a Nile blue dye, a cationic fullerene and a conjugate
between poly-L-lysine and chlorin(e6)) combined with appropriate wavelengths of light to inactivate C. neoformans.
Mutants lacking capsule and laccase, and culture conditions that favoured melanin production were used to probe the
mechanisms of PDI and the effect of virulence factors. The presence of cell wall, laccase and melanin tended to protect
against PDI, but the choice of the appropriate photosensitizers and dosimetry was able to overcome this resistance.
Como referenciar
PRATES, RENATO A.; FUCHS, BETH B.; MIZUMO, KAZUE; NAQVI, QURAT; KATO, ILKA T.; RIBEIRO, MARTHA S.; MYLONAKIS, ELEFTHERIOS; TEGOS, GEORGE P.; HAMBLIN, MICHAEL R. Effect of virulence factors on the photodynamic inactivation of Cryptococcus neoformans. PLoS ONE, v. 8, n. 1, p. e54387, 2013. DOI: 10.1371/journal.pone.0054387. Disponível em: http://repositorio.ipen.br/handle/123456789/8448. Acesso em: 16 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.