In vivo evaluation of genotoxic potential of liver cells from rats fed with irradiated diet using flow cytometry
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2017
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ANNUAL MEETING ON COUNCIL ON IONIZING RADIATION MEASUREMENTS AND STANDARDS, 25th
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Resumo
Radiation sources became widely available since 1960’s, and between its main uses are
the applications in food irradiation and research of effects of ionizing radiation on food
products. Despite some public concern, the process is safe, free from chemical residues
and presents advantages for preservation and storage. Nevertheless, safety dose
parameters must be adopted in irradiation procedures to inhibit formation of undesirable
and/or toxic products, for example, 2-ACB’s (2-alkylcyclobutanones) that are cyclic
compounds containing four carbon rings that can be formed in food when its fat content
is irradiated through breakdown of fatty acids. 2-ACBs are considered a unique class of
compounds due to divergences between results of its mutagenicity potential collected
from different studies. In this study, a cell population collected from rat livers were chosen
for in vivo genotoxicity analysis because the importance of the liver in the metabolization
of compounds. Analysis was performed using the micronuclei test using flow cytometry,
allowing faster analysis, use of few materials and reduction in the number of animals,
what is a subject much addressed currently in research. Irradiated rat diet did not show
any genotoxic effect on liver cell populations. The improvement of the techniques is
important for the future of the research since the irradiation process is already
consolidated.
Como referenciar
MARTINS, R.; CARVALHO, L.R. de; VIEIRA, D.P.; BARBEZAN, A.B.; VILLAVICENCIO, A.L.C.H. In vivo evaluation of genotoxic potential of liver cells from rats fed with irradiated diet using flow cytometry. In: ANNUAL MEETING ON COUNCIL ON IONIZING RADIATION MEASUREMENTS AND STANDARDS, 25th, March 27-29, 2017, Gaithersburg, MD, USA. Proceedings... Disponível em: http://repositorio.ipen.br/handle/123456789/28434. Acesso em: 20 Mar 2026.
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