Photodynamic effect of zinc porphyrin on the promastigote and amastigote forms of Leishmania braziliensis
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Photochemical & Photobiological Sciences
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Leishmaniasis is a neglected disease present in more than 88 countries. The currently adopted chemotherapy
faces challenges related to side effects and the development of resistance. Photodynamic
therapy (PDT) is emerging as a therapeutic modality for cutaneous leishmaniasis. Zn(II) meso-tetrakis(Nethylpyridinium-
2-yl)porphyrin (ZnTE-2-PyP4+, ZnP) is a cationic, water-soluble, zinc porphyrin-based
photosensitizer whose photodynamic effect on Leishmania braziliensis was analyzed by evaluating the
number of visibly undamaged and motile cells, cell membrane integrity, mitochondrial membrane potential,
and ultrastructural damage. Treatment of parasites with ZnP and light induced damage in up to 90%
of L. braziliensis promastigote cells. Propidium iodide labeling suggested the loss of plasma membrane
integrity. In samples treated with ZnP and light, a hyperpolarization of the mitochondrial membrane
potential was also observed. Ultrastructural evaluation of promastigotes after photodynamic treatment
indicated a loss of cytoplasmic material and the presence of vacuoles. Scanning electron microscopy
showed wrinkling of the plasma membrane and a reduced cell volume. Additionally, the number of amastigotes
per macrophage was reduced by about 40% after photodynamic application. The treatment
showed no considerable toxicity against mammalian cells. Therefore, the results indicated that PDT
associated with ZnTE-2-PyP4+ represents a promising alternative to cutaneous leishmaniasis treatment.
Como referenciar
ANDRADE, C.G.; FIGUEIREDO, R.C.B.Q.; RIBEIRO, K.R.C.; SOUZA, L.I.O.; SARMENTO-NETO, J.F.; REBOUCAS, J.S.; SANTOS, B.S.; RIBEIRO, M.S.; CARVALHO JUNIOR, L.B.; FONTES, A. Photodynamic effect of zinc porphyrin on the promastigote and amastigote forms of Leishmania braziliensis. Photochemical & Photobiological Sciences, v. 17, n. 4, p. 482-490, 2018. DOI: 10.1039/c7pp00458c. Disponível em: http://repositorio.ipen.br/handle/123456789/29062. Acesso em: 24 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.