Detection of Phakopsora pachyrhizi by polymerase chain reaction (PCR) and use of germination test and DNA comet assay afther e-beam processing in soybean
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Radiation Physics and Chemistry
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Soybean harvest is the main agribusiness in Brazil, which is the second largest exporter in the world and has a revenue of billions of
dollars. Asian dust is caused by the fungus Phakopsora pachyrhizi and its dissemination is difficult to control, since it occurs through
wind dispersion. Actually P. pachyrhizi is found in different parts of the world. Electron beam treatment could be an alternative process
to minimize these losses, especially for the grains exportation industry. Besides the possibility of being disconnected when not in use, this
source does not need to be reloaded, is easily available and, streamlines the process and reduces logistics costs. The present work aims to
identify, by the polymerase chain reaction technique (PCR), the P. pachyrhizi fungus presence in the irradiated soybeans and the
possibility to use radiation treatment as a sanitary alternative. Doses 0, 1.0, 2.0, 5.0, 6.0, 7.0, 8.0, 9.0 and 10.0 kGy (IPEN-CNEN/SP
Electron Accelerator) were applied and two fast-screening methods were performed: DNA comet assay (for the detection of DNA
damage) and germination test (for the measurement of roots inhibition). These tests are very easy to carry out and measure damage
response depending on radiation dose.
Como referenciar
VILLAVICENCIO, A.L.C.H.; FANARO, G.B.; ARAUJO, M.M.; AQUINO, S.; SILVA, P.V.; MANCINI FILHO, J. Detection of Phakopsora pachyrhizi by polymerase chain reaction (PCR) and use of germination test and DNA comet assay afther e-beam processing in soybean. Radiation Physics and Chemistry, v. 76, p. 1878-1881, 2007. DOI: 10.1016/j.radphyschem.2007.03.021. Disponível em: http://repositorio.ipen.br/handle/123456789/5157. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.