Red LED irradiation impacts the cytotoxic response of murine breast cancer cells to ionizing radiation
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2021
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CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th
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Breast cancer is a disease of worldwide importance since it is considered the 5th leading cause of cancer deaths.
Triple-negative breast cancer (TNBC) is a molecular subtype that presents resistance to conventional radiotherapy,
demanding high doses of ionizing radiation (IR) for a prolonged period of treatment. On the other hand, low-level light
irradiation (LLLI) has been studied to sensitize cells before IR exposure. However, the literature is poor regarding the
association of both techniques in TNBC cells. Thus, we aimed to assess the effect of LLLI before IR exposure on two
TNBC cell lineages. MDA-MB-231 (human TNBC) and 4T1 (murine TNBC) were cultivated, seeded at a density of
2.5 x 105 cells/cm², and maintained in an incubator (37ºC, 5% of CO2) overnight. LLLI was performed with a red LED
(λ= 660 ± 11 nm, 38.2 mW/cm²) delivering energies of 1.2 J and 6.0 J. One-h after LLLI, the cells were submitted to
both 2.5 and 5.0 Gy doses from a 60CO source. After 24-h, mitochondrial activity (MA) was quantified by MTT assay
with n= 9/group. Our data showed that 4T1 cells exposed to LLLI at 1.2 J exhibited higher MA than cells exposed to
IR2.5. In contrast, cells exposed to 6 J of LLLI showed lower MA than IR5. Concerning MDA-MB231 cells, no
statistically significant differences were noticed among groups regardless of IR and LLLI doses. These findings
indicate that LLLI before IR could sensitize only murine breast cancer. Besides, an appropriate combination of IR and
LLLI doses seems to play a role to kill TNBC cells.
Como referenciar
PINTO, MAYARA S.; SILVA, CAMILA R.; SALVEGO, CAMILA de A.; RIBEIRO, MARTHA S. Red LED irradiation impacts the cytotoxic response of murine breast cancer cells to ionizing radiation. In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 256-256. Disponível em: http://repositorio.ipen.br/handle/123456789/32872. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.