Biochemical changes in serum of obese mice related to photoactivation of brown adipose tissue
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2018
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CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOFISICA, 43.
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Obesity is closely related to the development of
insulin resistance and type-2 diabetes. Mammal fat
consists of white and brown adipose tissues (WAT
and BAT). Although most fat is energy-storing WAT,
brown adipocytes dissipate energy as heat. Thus, the
thermogenic capacity of BAT and its activation makes it an interesting target for treating metabolic syndrome
(MS). On the other hand, photobiomodulation (PBM)
has proven to be beneficial to manage the chronic
inflammatory component of obesity and hyperglycemia
by irradiating WAT. However, PBM has still not
been used to activate BAT. In this work, obese and
hyperglycemic mice were treated with PBM, and their
BAT was irradiated. The mouse serum was collected
and submitted to attenuated total reflection (ATR)-
Fourier transform infrared (FTIR) spectroscopy to
evaluate the biochemical changes promoted by PBM.
Five animals were fed with standard food (normal diet
– ND) and used as negative control. The remaining 10
animals were submitted to a hyperlipidic diet (high fat –
HF) and their body mass, Lee index and glycemia were
measured weekly during 15 weeks to induce MS. After
MS establishment, the HF animals were divided into two
groups: HF and HF660. The HF660 group was exposed
to six irradiation sessions using a 662 ± 20 nm LED.
The radiant exposure was 5.7 Jcm-2 at 19 mWcm-2
per session, delivered at 300 s, on days 1, 3, 7, 10,
14 and 21. The HF group was sham-irradiated. After
the treatment ended, the mouse serum was collected
from cardiac puncture under deep anesthesia. Serum
samples were prepared and analyzed by ATR-FTIR
spectroscopy. The lipid absorptions were responsible
for the clearest differences among the IR spectra.
An intense C=O stretching absorption at 1742 cm-1
was noticed only for HF group. Acyl CH2 stretching
absorptions at 2853 and 2925 cm−1 were also more
pronounced in HF group. After PBM, these major
lipid peaks decreased their vibrational modes. In fact,
hierarchical cluster analysis identified the similarities
between the spectra and grouped ND and HF660
groups into a same cluster. Taking together, our findings
suggest that PBM applied to the BAT is able to promote
biochemical changes in serum of obese mice mainly in
lipid bands.
Como referenciar
YOSHIMURA, T.M.; BAPTISTA, M.S.; RIBEIRO, M.S. Biochemical changes in serum of obese mice related to photoactivation of brown adipose tissue. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOFISICA, 43., 27-30 de setembro, 2018, Santos, SP. Resumo... Rio de Janeiro: Sociedade Brasileira de Biofísica, 2018. p. 35-35. Disponível em: http://repositorio.ipen.br/handle/123456789/29723. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.