A simple and quick method to generate in vitro tridimensional tumor bodies from a human breast adenocarcinoma (MCF7) using magnetic aggregation technique

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dc.contributor.authorLIMA, MAYELLE M.P.pt_BR
dc.contributor.authorNASCIMENTO, PAMELA F. dopt_BR
dc.contributor.authorNASCIMENTO, ANA C.G.pt_BR
dc.contributor.authorVIEIRA, DANIEL P.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoCONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13thpt_BR
dc.date.accessioned2022-03-25T19:46:00Z
dc.date.available2022-03-25T19:46:00Z
dc.date.eventoOctober 4-8, 2021pt_BR
dc.description.abstractTumor physiology studies have to rely on efficient and representative models, as animal-based or in vitro tridimensional cell constructs. The work used magnetite (Fe3O4) nanoparticles produced by electron-beam induced chemical reduction to give cells the ability to form aggregates when submitted to a magnetic field, and thus to produce micro tumors in vitro. The work aimed to produce human breast adenocarcinoma mini tumors (BAMT's) in vitro. Paramagnetic iron oxide nanoparticles (PION’s) were synthesized through electron-beam induced Fe3+ reduction and subsequent coprecipitation. Due to its poly-L-lysine coating, PION’s were adsorbed on cell membranes of MCF7 (human breast adenocarcinoma). Cells were seeded in 24-well cell culture plates pre-treated overnight with Pluronic® F-127 to prevent cell adhesion and kept in culture conditions under magnetic fields for at least 6 days. BAMT's were differentially stained with Hoescht 33342 and ethidium bromide and imaged by wide-field fluorescence microscopy. BAMT's appeared as integer and well-defined cellular aggregates, with sparse dead cells stained by ethidium bromide. These structures can be further used for in vitro tumor studies, as BAMT’s are supposed to be more reliable models than monolayer cultures. Treatment of wells with poloxamer caused a mild to moderated cell-repellent effect, similar to those found in commercially available products, only by a fraction of the cost. The experiments succesfully produced mini tumors prone to be used in in vitro studies.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP: 17/50332-0pt_BR
dc.event.siglaIUPAB; SBBq; SBBf; SBBNpt_BR
dc.format.extent382-382pt_BR
dc.identifier.citationLIMA, MAYELLE M.P.; NASCIMENTO, PAMELA F. do; NASCIMENTO, ANA C.G.; VIEIRA, DANIEL P. A simple and quick method to generate in vitro tridimensional tumor bodies from a human breast adenocarcinoma (MCF7) using magnetic aggregation technique. In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. <b>Abstract...</b> São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 382-382. Disponível em: http://repositorio.ipen.br/handle/123456789/32876.
dc.identifier.orcid0000-0002-0007-534Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32876
dc.localSão Paulo, SPpt_BR
dc.local.eventoSão Paulo, SPpt_BR
dc.publisherSociedade Brasileira de Bioquímica e Biologia Molecular (SBBq)pt_BR
dc.rightsopenAccesspt_BR
dc.subjectcarcinomas
dc.subjectmammary glands
dc.subjectnanoparticles
dc.subjectiron oxides
dc.titleA simple and quick method to generate in vitro tridimensional tumor bodies from a human breast adenocarcinoma (MCF7) using magnetic aggregation techniquept_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorPAMELA FERREIRA DO NASCIMENTO
ipen.autorDANIEL PEREZ VIEIRA
ipen.autorANA CRISTINA GOMES NASCIMENTO
ipen.autorMAYELLE MARIA PAZ LIMA
ipen.codigoautor15343
ipen.codigoautor3158
ipen.codigoautor14753
ipen.codigoautor14968
ipen.contributor.ipenauthorPAMELA FERREIRA DO NASCIMENTO
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.contributor.ipenauthorANA CRISTINA GOMES NASCIMENTO
ipen.contributor.ipenauthorMAYELLE MARIA PAZ LIMA
ipen.date.recebimento22-03
ipen.event.datapadronizada2021pt_BR
ipen.identifier.ipendoc28598pt_BR
ipen.notas.internasAbstractpt_BR
ipen.type.genreResumo
relation.isAuthorOfPublication82486792-9c8c-4d6e-aa27-d2e24704e42a
relation.isAuthorOfPublicationc2352608-be9c-4a73-be8c-571f10bb53d2
relation.isAuthorOfPublication12fd2681-92ad-4683-94d0-243825b4a97a
relation.isAuthorOfPublication83bccc9e-2167-4216-a6c2-b27567054fdc
relation.isAuthorOfPublication.latestForDiscovery83bccc9e-2167-4216-a6c2-b27567054fdc
sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:Npt_BR
sigepi.autor.atividadeNASCIMENTO, ANA C.G.:14753:810:Npt_BR
sigepi.autor.atividadeNASCIMENTO, PAMELA F. do:15343:810:Npt_BR
sigepi.autor.atividadeLIMA, MAYELLE M.P.:14968:810:Spt_BR
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