A simple and quick method to generate in vitro tridimensional tumor bodies from a human breast adenocarcinoma (MCF7) using magnetic aggregation technique
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2021
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CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th
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Tumor physiology studies have to rely on efficient and representative models, as animal-based or in vitro
tridimensional cell constructs. The work used magnetite (Fe3O4) nanoparticles produced by electron-beam induced
chemical reduction to give cells the ability to form aggregates when submitted to a magnetic field, and thus to produce
micro tumors in vitro. The work aimed to produce human breast adenocarcinoma mini tumors (BAMT's) in vitro.
Paramagnetic iron oxide nanoparticles (PION’s) were synthesized through electron-beam induced Fe3+ reduction
and subsequent coprecipitation. Due to its poly-L-lysine coating, PION’s were adsorbed on cell membranes of MCF7
(human breast adenocarcinoma). Cells were seeded in 24-well cell culture plates pre-treated overnight with Pluronic®
F-127 to prevent cell adhesion and kept in culture conditions under magnetic fields for at least 6 days. BAMT's were
differentially stained with Hoescht 33342 and ethidium bromide and imaged by wide-field fluorescence microscopy.
BAMT's appeared as integer and well-defined cellular aggregates, with sparse dead cells stained by ethidium
bromide. These structures can be further used for in vitro tumor studies, as BAMT’s are supposed to be more reliable
models than monolayer cultures. Treatment of wells with poloxamer caused a mild to moderated cell-repellent effect,
similar to those found in commercially available products, only by a fraction of the cost. The experiments succesfully
produced mini tumors prone to be used in in vitro studies.
Como referenciar
LIMA, MAYELLE M.P.; NASCIMENTO, PAMELA F. do; NASCIMENTO, ANA C.G.; VIEIRA, DANIEL P. A simple and quick method to generate in vitro tridimensional tumor bodies from a human breast adenocarcinoma (MCF7) using magnetic aggregation technique. In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 382-382. Disponível em: http://repositorio.ipen.br/handle/123456789/32876. Acesso em: 05 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.