Global priority multidrug-resistant pathogens do not resist photodynamic therapy
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Journal of Photochemistry and Photobiology B: Biology
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Microbial drug-resistance demands immediate implementation of novel therapeutic strategies. Antimicrobial
photodynamic therapy (aPDT) combines the administration of a photosensitizer (PS) compound with low-irradiance
light to induce photochemical reactions that yield reactive oxygen species (ROS). Since ROS react with
nearly all biomolecules, aPDT offers a powerful multitarget method to avoid selection of drug-resistant strains. In
this study, we assayed photodynamic inactivation under a standardized method, combining methylene blue
(MB) as PS and red light, against global priority pathogens. The species tested include Acinetobacter baumannii,
Klebsiella aerogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecium,
Enterococcus faecalis, Staphylococcus aureus, Candida albicans and Cryptococcus neoformans. Our strain collection
presents resistance to all tested antimicrobials (> 50). All drug-resistant strains were compared to their drugsensitive
counterparts. Regardless of resistance phenotype, MB-aPDT presented species-specific dose-response
kinetics. More than 5log10 reduction was observed within less than 75 s of illumination for A. baumannii, E. coli,
E. faecium, E. faecalis and S. aureus and within less than 7 min for K. aerogenes, K. pneumoniae, P. aeruginosa, C.
albicans and C. neoformans. No signs of correlations in between drug-resistance profiles and aPDT sensitivity
were observed. Therefore, MB-aPDT can provide effective therapeutic protocols for a very broad spectrum of
pathogens. Hence, we believe that this study represents a very important step to bring aPDT closer to implementation
into mainstream medical practices.
Como referenciar
SABINO, CAETANO P.; WAINWRIGHT, MARK; RIBEIRO, MARTHA S.; SELLERA, FABIO P.; ANJOS, CAROLINA dos; BAPTISTA, MAURICIO da S.; LINCOPAN, NILTON. Global priority multidrug-resistant pathogens do not resist photodynamic therapy. Journal of Photochemistry and Photobiology B: Biology, v. 208, p. 1-9, 2020. DOI: 10.1016/j.jphotobiol.2020.111893. Disponível em: http://repositorio.ipen.br/handle/123456789/31380. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.