Micro-Raman spectroscopy characterization of dental pulp stem cells differentiation induced by calcium phosphate nanoparticles
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2022
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ENCONTRO BRASILEIRO DE ESPECTROSCOPIA RAMAN, 7.
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Calcium phosphates are chemical compounds used in medicine for tissue engineering. This work analyzes the
process of cell differentiation by nanoparticles in dental pulp stem cells for tissues regeneration and the development
of new therapeutic methods. The most widely used synthetic calcium phosphate based bioceramic is hydroxyapatite
[HA, Ca10(PO4)6(OH)2]. Micro-Raman spectroscopy assays are a powerful tool for measuring and characterizing
calcium phosphates nanoparticles internalized by cells because of its capability to detect the chemical bonds of
nanoparticles and collagen simultaneously and evidencing their interaction within the cell-nanoparticle system.
Microscope images (Fig.1a) and Raman spectra (Fig.1b) were obtained for HA-incorporated stem cells where it was
possible to observe the formed nodules of calcium phosphate and the matrix in the incorporated samples. HA and
collagen peaks were detected in the samples, showing that the nanoparticles induced osteogenic differentiation of
the stem cells. The spectra of the nodules showed HA characteristic peaks, while matrix spectra displayed
characteristic collagen peaks. Studies have been carried out for the development of new and modified calcium
phosphate nanoparticles that should further improve biostimulation.
Como referenciar
SILVA, FLAVIA R.O.; PASCOAL, DIEGO R.C.; BERECZKI, ALLAN; SIPERT, CARLA R.; BRAGA, ROBERTO R.; BELLINI, MARIA H.; SILVA, LUIS F.T. da; FREITAS, ANDERSON Z.; WETTER, NIKLAUS U. Micro-Raman spectroscopy characterization of dental pulp stem cells differentiation induced by calcium phosphate nanoparticles. In: ENCONTRO BRASILEIRO DE ESPECTROSCOPIA RAMAN, 7., 5-8 de dezembro, 2022, São Pedro, SP. Resumo... Disponível em: http://repositorio.ipen.br/handle/123456789/33684. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.