Micro-Raman spectroscopy characterization of dental pulp stem cells differentiation induced by calcium phosphate nanoparticles

Página do item simplificado
dc.contributor.authorSILVA, FLAVIA R.O.pt_BR
dc.contributor.authorPASCOAL, DIEGO R.C.pt_BR
dc.contributor.authorBERECZKI, ALLANpt_BR
dc.contributor.authorSIPERT, CARLA R.pt_BR
dc.contributor.authorBRAGA, ROBERTO R.pt_BR
dc.contributor.authorBELLINI, MARIA H.pt_BR
dc.contributor.authorSILVA, LUIS F.T. dapt_BR
dc.contributor.authorFREITAS, ANDERSON Z.pt_BR
dc.contributor.authorWETTER, NIKLAUS U.pt_BR
dc.coverageNacionalpt_BR
dc.creator.eventoENCONTRO BRASILEIRO DE ESPECTROSCOPIA RAMAN, 7.pt_BR
dc.date.accessioned2023-02-01T11:36:45Z
dc.date.available2023-02-01T11:36:45Z
dc.date.evento5-8 de dezembro, 2022pt_BR
dc.description.abstractCalcium phosphates are chemical compounds used in medicine for tissue engineering. This work analyzes the process of cell differentiation by nanoparticles in dental pulp stem cells for tissues regeneration and the development of new therapeutic methods. The most widely used synthetic calcium phosphate based bioceramic is hydroxyapatite [HA, Ca10(PO4)6(OH)2]. Micro-Raman spectroscopy assays are a powerful tool for measuring and characterizing calcium phosphates nanoparticles internalized by cells because of its capability to detect the chemical bonds of nanoparticles and collagen simultaneously and evidencing their interaction within the cell-nanoparticle system. Microscope images (Fig.1a) and Raman spectra (Fig.1b) were obtained for HA-incorporated stem cells where it was possible to observe the formed nodules of calcium phosphate and the matrix in the incorporated samples. HA and collagen peaks were detected in the samples, showing that the nanoparticles induced osteogenic differentiation of the stem cells. The spectra of the nodules showed HA characteristic peaks, while matrix spectra displayed characteristic collagen peaks. Studies have been carried out for the development of new and modified calcium phosphate nanoparticles that should further improve biostimulation.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP: 16/02326-9; 17/10765-5; 18/19240-5; 19/06334-4; 21/11316-5; 22/07199-6pt_BR
dc.event.siglaEnBraERpt_BR
dc.identifier.citationSILVA, FLAVIA R.O.; PASCOAL, DIEGO R.C.; BERECZKI, ALLAN; SIPERT, CARLA R.; BRAGA, ROBERTO R.; BELLINI, MARIA H.; SILVA, LUIS F.T. da; FREITAS, ANDERSON Z.; WETTER, NIKLAUS U. Micro-Raman spectroscopy characterization of dental pulp stem cells differentiation induced by calcium phosphate nanoparticles. In: ENCONTRO BRASILEIRO DE ESPECTROSCOPIA RAMAN, 7., 5-8 de dezembro, 2022, São Pedro, SP. <b>Resumo...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/33684.
dc.identifier.orcid0000-0002-9379-9530pt_BR
dc.identifier.orcid0000-0002-5018-9126pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9379-9530
dc.identifier.orcidhttps://orcid.org/0000-0002-5018-9126
dc.identifier.orcidhttps://orcid.org/0000-0003-2852-6189
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/33684
dc.local.eventoSão Pedro, SPpt_BR
dc.rightsopenAccesspt_BR
dc.titleMicro-Raman spectroscopy characterization of dental pulp stem cells differentiation induced by calcium phosphate nanoparticlespt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorDIEGO ROBERTO DA CUNHA PASCOAL
ipen.autorALLAN BERECZKI
ipen.autorNIKLAUS URSUS WETTER
ipen.autorANDERSON ZANARDI DE FREITAS
ipen.autorLUIZ FELIPE TEIXEIRA DA SILVA
ipen.autorMARIA HELENA BELLINI MARUMO
ipen.autorFLAVIA RODRIGUES DE OLIVEIRA SILVA
ipen.codigoautor15807
ipen.codigoautor12080
ipen.codigoautor919
ipen.codigoautor880
ipen.codigoautor14146
ipen.codigoautor1242
ipen.codigoautor5910
ipen.contributor.ipenauthorDIEGO ROBERTO DA CUNHA PASCOAL
ipen.contributor.ipenauthorALLAN BERECZKI
ipen.contributor.ipenauthorNIKLAUS URSUS WETTER
ipen.contributor.ipenauthorANDERSON ZANARDI DE FREITAS
ipen.contributor.ipenauthorLUIZ FELIPE TEIXEIRA DA SILVA
ipen.contributor.ipenauthorMARIA HELENA BELLINI MARUMO
ipen.contributor.ipenauthorFLAVIA RODRIGUES DE OLIVEIRA SILVA
ipen.date.recebimento23-02
ipen.event.datapadronizada2022pt_BR
ipen.identifier.ipendoc29318pt_BR
ipen.notas.internasResumopt_BR
ipen.type.genreResumo
relation.isAuthorOfPublicationb349e332-17e2-418c-bf10-855c9f63aa5a
relation.isAuthorOfPublicationc1a4f374-98c2-4d09-a07b-df64015613ed
relation.isAuthorOfPublication464db0c6-6072-480b-b899-81848893f7eb
relation.isAuthorOfPublicationa8d821f0-a0a4-4d52-b582-5c5d0afce8f5
relation.isAuthorOfPublication1ccbe3d6-1fec-40ee-9090-54f8ccd43c4d
relation.isAuthorOfPublication6a450cbf-91b1-4386-b4a8-7304afac99cc
relation.isAuthorOfPublicationb1aa6134-5ebc-46f0-ada3-605eaec3a986
relation.isAuthorOfPublication.latestForDiscoveryb1aa6134-5ebc-46f0-ada3-605eaec3a986
sigepi.autor.atividadeWETTER, NIKLAUS U.:919:910:Npt_BR
sigepi.autor.atividadeFREITAS, ANDERSON Z.:880:920:Npt_BR
sigepi.autor.atividadeSILVA, LUIS F.T. da:14146:810:Npt_BR
sigepi.autor.atividadeBELLINI, MARIA H.:1242:810:Npt_BR
sigepi.autor.atividadeBERECZKI, ALLAN:12080:910:Npt_BR
sigepi.autor.atividadeSILVA, FLAVIA R.O.:5910:810:Spt_BR
sigepi.autor.atividadePASCOAL, DIEGO R.C.:15807:910:N
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
29318.pdf
Tamanho:
251.53 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar
Coleções
Eventos - Resumos