Study of blood porphyrin spectral profile for diagnosis of chronic renal failure
Carregando...
Data
2010
Data de publicação:
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
Journal of Fluorescence
Exportar
Resumo
The progression to end-stage renal failure is independent of the initial pathogenic mechanism. Metabolic acidosis is a common consequence of chronic renal failure that results from inadequate ammonium excretion and decreased tubular bicarbonate reabsorption. Protoporphyrin IX (PpIX) is the immediate metabolic precursor of the heme molecule. The purpose of this study was to evaluate the levels of erythrocytes protoporphyrin IX at an animal model during progressive renal disease. A total of 36 eightweek-old male Wistar rats were divided into six groups: Normal, 4 and 8 weeks after 5/6 nephrectomy (NX). Renal function was evaluated by creatinine clearance and plasma creatinine levels. The autofluorescence of erythrocytes porphyrin of healthy and NX rats was analyzed using fluorescence spectroscopy. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences between normal and NX rats autofluorescence shape occurred in the 600–700 nm spectral region. A correlation was observed between emission band intensity at 635 nm and progression of renal disease.
Como referenciar
TRISTAO, VIVIAN R.; CARVALHO, FERNANDO F. de; GOMES, CINTHIA Z.; MIRANDA, ADRIANA R.; VEQUI-SUPLICY, CINTIA C.; LAMY, MARIA T.; SCHOR, NESTOR; BELLINI, MARIA H. Study of blood porphyrin spectral profile for diagnosis of chronic renal failure. Journal of Fluorescence, v. 20, n. 3, p. 665-669, 2010. DOI: 10.1007/s10895-010-0600-x. Disponível em: http://repositorio.ipen.br/handle/123456789/4606. Acesso em: 23 Feb 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.