Development of radiation-induced albumin-based nanoparticles
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2023
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LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th
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Introduction and objective: Proteins have been the subject of studies in nanotechnology because they have
properties such as high biocompatibility and low toxicity. In this work, we emphasize albumin for the
development of nanostructured systems developed by radiation-induced cross-linking [1], intending to deliver
melatonin for antitumor purposes, as well as determining its morphological, physicochemical characteristics
and evaluating the profile on normal and tumor cells. The development of the study is significant, as new
strategies based on nanoparticles make it possible to combat existing challenges in conventional therapy in
the treatment of cancer, along with improvements in targeting drugs in the tumor.
Methodology: The synthesis was carried out in the presence of ethanol (30%, v/v) and variation in protein
concentration from 0.5 to 10mg/mL in different buffers (phosphate and tris-HCl), both at a concentration of
50mM and gamma radiation (1-20 kGy) for nanoparticle formation. The samples were evaluated using the
dynamic light scattering technique assess the hydrodynamic diameter, infrared spectroscopy, and
fluorescence. The encapsulation efficiency was made by high-performance liquid chromatography, and the
cytotoxicity of the nanoparticles was evaluated for the proliferation in different tumor cells.
Results and discussion: In this work, albumin nanoparticles were developed containing melatonin, aiming for
better melatonin availability related to the free drug. The use of radiation for cross-linking of nanoparticles
has advantages since it is not restricted to the lack of monomers in the process, and, additionally, the absence
of cross-linking agents ensures low residual toxicity and reduces possible purification steps of remaining
monomers. Through the characterization analysis of the nanoparticles, it was possible to observe that the
average diameter obtained varies from 30-60nm. It was possible to analyze that the increase in the size of the
nanoparticles is directly linked to the change of buffer, albumin concentration, and irradiation dose, the
average encapsulation efficiency was above 50%, the nanoparticles remained stable for at least 60 days, both
at room temperature and in a refrigerated environment. The analyzed nanoparticles did not demonstrate a
cytotoxicity profile in healthy cells.
Conclusions: The development protocol of the nanostructured system presented nanoparticles with desirable
sizes and stability in the time studied and also observed the efficiency of incorporation of the drug in the
nanoparticles, demonstrating promising nanocarriers for the loading of radiopharmaceuticals and
physicochemical compatible with the nanostructured systems for application biological.
Como referenciar
CRUZ, CASSIA P.C. da; LEBRE, DANIEL T.; SANTOS, LARRISSA S. dos; LUGAO, ADEMAR B. Development of radiation-induced albumin-based nanoparticles. In: LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th, December 12-15, 2023, Mar del Plata, Argentina. Abstract... p. 238. Disponível em: https://repositorio.ipen.br/handle/123456789/47928. Acesso em: 04 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.