Development of radiation-induced albumin-based nanoparticles

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dc.contributor.authorCRUZ, CASSIA P.C. da
dc.contributor.authorLEBRE, DANIEL T.
dc.contributor.authorSANTOS, LARRISSA S. dos
dc.contributor.authorLUGAO, ADEMAR B.
dc.coverageInternacional
dc.creator.eventoLATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th
dc.date.accessioned2024-03-14T15:24:46Z
dc.date.available2024-03-14T15:24:46Z
dc.date.eventoDecember 12-15, 2023
dc.description.abstractIntroduction and objective: Proteins have been the subject of studies in nanotechnology because they have properties such as high biocompatibility and low toxicity. In this work, we emphasize albumin for the development of nanostructured systems developed by radiation-induced cross-linking [1], intending to deliver melatonin for antitumor purposes, as well as determining its morphological, physicochemical characteristics and evaluating the profile on normal and tumor cells. The development of the study is significant, as new strategies based on nanoparticles make it possible to combat existing challenges in conventional therapy in the treatment of cancer, along with improvements in targeting drugs in the tumor. Methodology: The synthesis was carried out in the presence of ethanol (30%, v/v) and variation in protein concentration from 0.5 to 10mg/mL in different buffers (phosphate and tris-HCl), both at a concentration of 50mM and gamma radiation (1-20 kGy) for nanoparticle formation. The samples were evaluated using the dynamic light scattering technique assess the hydrodynamic diameter, infrared spectroscopy, and fluorescence. The encapsulation efficiency was made by high-performance liquid chromatography, and the cytotoxicity of the nanoparticles was evaluated for the proliferation in different tumor cells. Results and discussion: In this work, albumin nanoparticles were developed containing melatonin, aiming for better melatonin availability related to the free drug. The use of radiation for cross-linking of nanoparticles has advantages since it is not restricted to the lack of monomers in the process, and, additionally, the absence of cross-linking agents ensures low residual toxicity and reduces possible purification steps of remaining monomers. Through the characterization analysis of the nanoparticles, it was possible to observe that the average diameter obtained varies from 30-60nm. It was possible to analyze that the increase in the size of the nanoparticles is directly linked to the change of buffer, albumin concentration, and irradiation dose, the average encapsulation efficiency was above 50%, the nanoparticles remained stable for at least 60 days, both at room temperature and in a refrigerated environment. The analyzed nanoparticles did not demonstrate a cytotoxicity profile in healthy cells. Conclusions: The development protocol of the nanostructured system presented nanoparticles with desirable sizes and stability in the time studied and also observed the efficiency of incorporation of the drug in the nanoparticles, demonstrating promising nanocarriers for the loading of radiopharmaceuticals and physicochemical compatible with the nanostructured systems for application biological.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDCAPES: 001
dc.description.sponsorshipIDFAPESP: 17/50332-0
dc.event.siglaCOLAOB
dc.format.extent238-238
dc.identifier.citationCRUZ, CASSIA P.C. da; LEBRE, DANIEL T.; SANTOS, LARRISSA S. dos; LUGAO, ADEMAR B. Development of radiation-induced albumin-based nanoparticles. In: LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th, December 12-15, 2023, Mar del Plata, Argentina. <b>Abstract...</b> p. 238. Disponível em: https://repositorio.ipen.br/handle/123456789/47928.
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.urihttps://repositorio.ipen.br/handle/123456789/47928
dc.local.eventoMar del Plata, Argentina
dc.titleDevelopment of radiation-induced albumin-based nanoparticles
dc.typeResumo de eventos científicos
dspace.entity.typePublication
ipen.autorCASSIA PRISCILA CUNHA DA CRUZ
ipen.autorDANIEL TEMPONI LEBRE
ipen.autorLARISSA SABINO DOS SANTOS
ipen.autorADEMAR BENEVOLO LUGAO
ipen.codigoautor15070
ipen.codigoautor2053
ipen.codigoautor15667
ipen.codigoautor339
ipen.contributor.ipenauthorCASSIA PRISCILA CUNHA DA CRUZ
ipen.contributor.ipenauthorDANIEL TEMPONI LEBRE
ipen.contributor.ipenauthorLARISSA SABINO DOS SANTOS
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.event.datapadronizada2023
ipen.identifier.ipendoc30276
ipen.notas.internasAbstract
ipen.type.genreResumo
relation.isAuthorOfPublication296b0b7e-28f4-4658-b7d4-f52e0494fe8f
relation.isAuthorOfPublicationb1305c93-be39-477e-97ae-bd94c730015d
relation.isAuthorOfPublicationfde3378b-75a1-407d-84fd-5e26fcc6f739
relation.isAuthorOfPublication99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
relation.isAuthorOfPublication.latestForDiscovery296b0b7e-28f4-4658-b7d4-f52e0494fe8f
sigepi.autor.atividadeCRUZ, CASSIA P.C. da:15070:750:S
sigepi.autor.atividadeLEBRE, DANIEL T.:2053:520:N
sigepi.autor.atividadeSANTOS, LARRISSA S. dos:15667:750:N
sigepi.autor.atividadeLUGAO, ADEMAR B.:339:740:N
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