Evaluation of combined photodynamic inactivation and no releasing chitosan nanoparticles on cutaneous leishmaniasis
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2018
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CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOFISICA, 43.
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Resumo
Cutaneous leishmaniasis (CL) is a chronic disease
developed by parasites of the genus Leishmania that
promotes destructive lesions. The available treatments
are limited because of side effects, resistance and
toxicity. New strategies against CL have been studied
such as Photodynamic inactivation (PDI) and exogenous
NO donors. The aim of this work was to explore the effects
of methylene blue (MB)-mediated PDI in association
with encapsulated NO donors in chitosan nanoparticles
(NPNO) on Leishmania amazonensis. NPNOs were tested
in vitro with L. amazonensis transgenic line expressing
luciferase at increasing concentrations (25-200μM)
and inhibitory concentrations (IC50 and IC90 ) were
calculated. Based on inhibitory concentrations results,
twelve BALB/c mice were infected in the left footpad
and randomly assigned to experimental groups (n=4):
Control (non-treated), G1 (two PDI sessions), G2 (two
PDI sessions and 80 μM of NPNO, immediately after
PDI) and G3 (only 80 μM NPNO). PDI was performed
using a red LED (λ= 660±22 nm) at 150 J/cm² fluence
and MB at 100 μM. Parasite burden was obtained by
bioluminescence every day, in the first 96 h and for
the next 4 weeks, once a week. Test groups presented
significant reduction in parasite load compared to
control during all experimental period. In the first 24 h
after treatments, parasite burden was significant lower
for G2. After 96 h, all test groups were similar. Following
4 weeks, statistically significant differences were
noticed when test groups were compared to control but
parasite burden was similar among all treated groups.
Under conditions used in this study, our results show
that NPNOs were not able to sustain the parasite killing
promoted by MB-mediated PDI on CL induced in mice
24 h after treatments.
Como referenciar
PELEGRINO, M.T.; DIMMER, J.A.; SEABRA, A.B.; RIBEIRO, M.S.; CABRAL, F.V. Evaluation of combined photodynamic inactivation and no releasing chitosan nanoparticles on cutaneous leishmaniasis. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOFISICA, 43., 27-30 de setembro, 2018, Santos, SP. Resumo... Rio de Janeiro: Sociedade Brasileira de Biofísica, 2018. p. 64-64. Disponível em: http://repositorio.ipen.br/handle/123456789/29724. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.