Effects of methylene blue-mediated photodynamic inactivation associated to NO-releasing chitosan nanoparticles on cutaneous leishmaniasis in mice
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2017
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INTERNATIONAL PHOTODYNAMIC ASSOCIATION WORLD CONGRESS, 16th
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Cutaneous leishmaniasis (C L) is a ehronie disease developed by parasites of the genus
Leishmania that promotes destruetive.and ulcerated lesions. The available treatments are limited
beeause of side effeets, resistanee and toxieity. Reaetive oxygen speeies and nitrie oxide (NO)
are potentially toxic to these parasites; Photodynamie inaetivation (PDI) involves the generation
af oxidative stress and has been explored as an altemative treatment once it is less expensive
and no reports about resistanee have been describedY Additionally, several studies indicate that
the administration of exogenous NO donors represents an interesting strategy against CL.3 The
aim of this work was to explore the effects Df methylene blue (MB)-mediated PDI in assoeiation
with encapsulated NO donors (S-nitroso-MSA) in chitosan nanoparticles (CSNPs) on CL in
BALB/e mice using real time bioluminescence.
Promastigotes of L. (L) amazonensis transgenie line expressing luciferase were used. Sixteen
BALB/e miee were infected in the left footpad with 1.106 promastigotes. After 4 weeks, mice
were randomly assigned to experimental groups (n=4): Control (non-treated), PDI (treated only
with PDI), PDI+CSNP (submitted to PDI and S-nitroso-MSA-CSNPs) and CSNP (treated only
with S-nitroso-MSA-CSNPs). PDI was administered in two sessions separated by 24 h and
CSNPs (80 eM) were applied immediately after the second PDI session. PDI was performed
using a red LED (0= 660 ± 22 nm), MB (100 IlM), irradiance of 100 mW/cm2 and radiant
exposure of 150 J/cm2
• parasite burden was analyzed through luciferase deteetion by bioimaging
in the first 96 h following treatment and every week during 4 weeks. Statistically significant
differences were considered when p < 0.05.
Test groups presented significant reduction in parasite load compared to control during all
experimental period. Twenty-four-h after treatments, parasite burden was lower for PDI+CSNP
group but no statistically signifieant difference was observed when compared to other test
groups. After 48 h, all test groups were similar. Besides, parasite load in test groups remained
lower than control following 1, 2,3 and 4 weeks post-treatment.
Under conditions used in this study, we conclude that CSNPs were not able to enhance MBmediated
PDI efficiency in L. (L) amazonensis-induced CL in mice.
Como referenciar
CABRAL, F.V.; PELEGRINO, M.T.; SEABRA, A.B.; RIBEIRO, M.S. Effects of methylene blue-mediated photodynamic inactivation associated to NO-releasing chitosan nanoparticles on cutaneous leishmaniasis in mice. In: INTERNATIONAL PHOTODYNAMIC ASSOCIATION WORLD CONGRESS, 16th, June 08-13, 2017, Coimbra, Portugal. Abstract... p. 272-272. Disponível em: http://repositorio.ipen.br/handle/123456789/29244. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.