Low power light triggers opposite effects on stem cells
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2021
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CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th
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Photobiomodulation (PBM) has been gaining importance in a wide range of medical fields in the past few
years, particularly in stem cell-based regenerative medicine. Improving in vitro cell proliferation,
differentiation and viability are ways where PBM could play a pivotal role optimizing biotechnological and
bioengineering applications. Here we investigated whether different wavelengths (blue, green and red)
would promote distinct outcomes in human adipose-derived stem cells (hADSCs) cultured in regular and
supplemented media for tenocyte differentiation. MATERIALS AND METHODS. Freshly isolated hADSCs
were cultured in a specific stem cell medium (MSCGM, Lonza), DMEM or a tenogenic medium (TEN-M:
DMEM supplemented with growth factors and ascorbic acid). Cells were irradiated every 48 h (23.28
mW/cm 2, 17 min 10 s delivering 24 J/cm 2 per session) using a LED irradiator (LEDbox, BioLambda).
MTT and crystal violet assays were used to evaluate cell metabolic activity and proliferation. Red
wavelength (660 nm) significantly increased metabolic activity after five irradiations, but only for cells
cultured in TEN-M. Oppositely, blue (450 nm) and green (520 nm) light decreased both cell proliferation
and metabolic rate, with more pronounced effects for blue light in TEN-M. Considering these findings, we
examined whether irradiating only the media would generate toxic compounds that could impair cell
viability. We therefore assessed reactive oxygen species (ROS) production by p-nitrosodimethylaniline/
histidine assay while irradiating the three different media under the same conditions
as mentioned above. Immediately after blue and green light exposure, an increment in ROS production
was observed for DMEM and TEN-M, that continuously increased until reaching between 4.5 and 7.1 μM
one-hour after irradiation – with higher values for TEN-M exposed to blue light. Since no significant ROS
formation was observed following red light exposure, we concluded that medium composition was
responsible for the different effects on metabolic activity and proliferation observed after irradiation with
different wavelengths.
Como referenciar
YOSHIMURA, TANIA M.; SAUTER, ISMAEL P.; RIBEIRO, MARTHA S. Low power light triggers opposite effects on stem cells: influence of the wavelength and culture conditions. In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 41-41. Disponível em: http://repositorio.ipen.br/handle/123456789/32861. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.