Candida albicans biofilm alterations during photodynamic therapy by optical coherence tomography video monitoring
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2010
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ENCONTRO NACIONAL DE FISICA DA MATERIA CONDENSADA, 33.
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Reports in literature show
that photodynamic therapy (PDT) presents a lethal
effect on fungi, e.g. Candida albicans. It is based on
the use of a photosensitizer (PS) in the presence of low
intensity light to generate reactive oxygen species in
biological systems. The purpose of this study was to
analyze modifications in C. albicans biofilm in vitro
during PDT using methylene blue (MB) and red light,
with real time Optical Coherence Tomography (OCT).
The OCT system used 930nm with 2mW of power and
sequential images of 2000x512 pixels were generated
at the frame rate of 2.5 frames/sec. The dimension of
the analyzed sample was 6000 microns wide by 1170
microns of depth corrected by refraction index of 1.35.
Images were recorded 1min before and 1min after the
irradiation with LED for PDT, generating 1,200 images
producing 8min of video. For biofilm growth, discs were
made from elastomeric silicone catheters. The PS was
dissolved in PBS solution, and a final concentration
of 1mM MB was applied on the biofilm. A red LED
(wavelenght = 630nm ± 20nm) was used for biofilm
irradiation. We performed a curve of survival fraction
versus time of irradiation and it was reduced by 100%
following 6min of irradiation. OCT was performed for measurement of biofilm thickness, and it was 110
microns in the end time of biofilm formation. During
the irradiation, the biofilm thickness changed in 70
microns. We conclude that OCT system is capable to
show real time optical changes in yeasts organized in
biofilm simultaneous to PDT.
Como referenciar
FREITAS, ANDERSON Z. de; SUZUKI, LUIS C.; PRATES, RENATO A.; RAELE, MARCUS P.; AMARAL, MARCELLO M.; RIBEIRO, MARTHA S. Candida albicans biofilm alterations during photodynamic therapy by optical coherence tomography video monitoring. In: ENCONTRO NACIONAL DE FISICA DA MATERIA CONDENSADA, 33., 10-14 de maio, 2010, Aguas de Lindoia, SP. Resumos... Disponível em: http://repositorio.ipen.br/handle/123456789/21205. Acesso em: 30 Dec 2025.
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