Exploring the effects of low-level laser therapy on fibroblasts and tumor cells following gamma radiation exposure
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Journal of Biophotonics
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Ionizing radiation (IR) induces DNA damage and low-level
laser therapy (LLLT) has been investigated to prevent
or repair detrimental outcomes resulting from IR exposure.
Few in vitro studies, however, explore the biological
mechanisms underlying those LLLT benefits. Thus, in this
work, fibroblasts and tumor cells are submitted to IR with
doses of 2.5 Gy and 10 Gy. After twenty-four-h, the cells
are exposed to LLLT with fluences of 30 J cm–2, 90 J cm–2,
and 150 J cm–2. Cellular viability, cell cycle phases, cell
proliferation index and senescence are evaluated on days
1 and 4 after LLLT irradiation. For fibroblasts, LLLT
promotes – in a fluence-dependent manner – increments
in cell viability and proliferation, while a reduction in the
senescence was observed. Regarding tumor cells, no influences
of LLLT on cell viability are noticed. Whereas
LLLT enhances cell populations in S and G2/M cell cycle
phases for both cellular lines, a decrease in proliferation
and increase in senescence was verified only for tumor
cells. Putting together, the results suggest that fibroblasts
and tumor cells present different responses to LLLT following
exposure to gamma-radiation, and these promising
results should stimulate further investigations.
Senescence of tumor cells and fibroblasts on the 4th day
after ionizing radiation (IR) and low-level laser therapy
(LLLT) exposures. The number of senescent cells increased
significantly for tumor cells (a) while for fibroblasts
no increment was observed (b). The blue collor indicates
senescence activity.
Como referenciar
SILVA, CAMILA R.; CABRAL, FERNANDA V.; CAMARGO, CLAUDINEI F.M. de; NUNEZ, SILVIA C.; YOSHIMURA, TANIA M.; LUNA, ARTHUR C. de L.; MARIA, DURVANEI A.; RIBEIRO, MARTHA S. Exploring the effects of low-level laser therapy on fibroblasts and tumor cells following gamma radiation exposure. Journal of Biophotonics, v. 9, n. 11-12, p. 1157-1166, 2016. DOI: 10.1002/jbio.201600107. Disponível em: http://repositorio.ipen.br/handle/123456789/27214. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.