Production of a double-network hydrogel using sodium alginate and nano-structured cellulose to 3D cell cultures
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2023
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LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th
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Introduction and objective
2D cell cultures have limitations regarding on tissue representativity. 3D cell cultures can use hydrogels of
alginate with cellulose with adequate viscoelasticity properties for cell growth, being from plant sources,
abundant and low cost. This work consisted of producing a biocompatible gel from plant sources for threedimensional cultures, promoting polymeric matrices for cells, helping in cell interactions and nutrient
transport, providing mechanical support, self-assembly capacity, biodegradation, ability to reticulation,
stability control and mechanical resistance.
Methodology
Transformation of microcrystalline cellulose into nanofibers was achieved freezing aqueous suspensions on
presence of 4M NaOH to proper dissociation of fibers. To obtain suitable dispersion, sodium citrate was
added to prevent aggregation. Suspensions were analyzed by Scanning Electron Microscope (SEM), Fourier
Transform Infrared Spectroscopy (FTIR), Zeta Potential. For cell viability analysis, murine fibroblastic cell lines
(NIH/3T3) were plated (2.5 x 105 cells per well) 24-well plate embedded in gel (100µL).
Results and discussion
The analysis of cellulose suspensions through SEM, showed a significant change in the size and shape of the
structures after hydrolysis, indicating the obtention of structures on a nanometric scale. For the analysis of
cellulose aggregation, the zeta potential values indicated that after the addition of sodium citrate, greater
dispersion was obtained between the cellulose structures, enabling resistance to the structure in a uniform
way. FTIR analysis showed changes in the covalent bonds of the products. Cell viability assay showed
structures containing fibroblast cells, alginate and cellulose with 1 cycle of freezing with citrate showed an
intact gel structure, with cell aggregates indicating possible cell growth, while the one with only alginate
showed dead cells and showed that the hydrogel did not induce cellular toxicity. These results suggest that
the hydrolysis of microcrystalline cellulose can lead to obtaining cellulose nanofibers with potential for
applications in tissue engineering.
Conclusions
Hydrogels, they have potential for applications in tissue engineering, since they have mechanical resistance
and cell viability. In addition, hydrogels from exclusively vegetable sources, since these are in large quantity,
low cost and environmental impact, given that the alginate comes from brown algae found in several coastal
regions and the cellulose can be extracted from renewable sources or various vegetable waste from
agroindustry.
Como referenciar
SILVA, GIOVANA D. da; SAKATA, SOLANGE K.; ASSIS, JOAO V.A. de; SANTOS, ESTHER C. dos; PRUDENTE, SULEYNA R.; RODRIGUES, ALEX A.; FALCAO, PATRICIA L.; VIEIRA, DANIEL P. Production of a double-network hydrogel using sodium alginate and nano-structured cellulose to 3D cell cultures. In: LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th, December 12-15, 2023, Mar del Plata, Argentina. Abstract... p. 76-76. Disponível em: https://repositorio.ipen.br/handle/123456789/47951. Acesso em: 06 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.