Production of a double-network hydrogel using sodium alginate and nano-structured cellulose to 3D cell cultures

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dc.contributor.authorSILVA, GIOVANA D. da
dc.contributor.authorSAKATA, SOLANGE K.
dc.contributor.authorASSIS, JOAO V.A. de
dc.contributor.authorSANTOS, ESTHER C. dos
dc.contributor.authorPRUDENTE, SULEYNA R.
dc.contributor.authorRODRIGUES, ALEX A.
dc.contributor.authorFALCAO, PATRICIA L.
dc.contributor.authorVIEIRA, DANIEL P.
dc.coverageInternacional
dc.creator.eventoLATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th
dc.date.accessioned2024-03-15T17:29:43Z
dc.date.available2024-03-15T17:29:43Z
dc.date.eventoDecember 12-15, 2023
dc.description.abstractIntroduction and objective 2D cell cultures have limitations regarding on tissue representativity. 3D cell cultures can use hydrogels of alginate with cellulose with adequate viscoelasticity properties for cell growth, being from plant sources, abundant and low cost. This work consisted of producing a biocompatible gel from plant sources for threedimensional cultures, promoting polymeric matrices for cells, helping in cell interactions and nutrient transport, providing mechanical support, self-assembly capacity, biodegradation, ability to reticulation, stability control and mechanical resistance. Methodology Transformation of microcrystalline cellulose into nanofibers was achieved freezing aqueous suspensions on presence of 4M NaOH to proper dissociation of fibers. To obtain suitable dispersion, sodium citrate was added to prevent aggregation. Suspensions were analyzed by Scanning Electron Microscope (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Zeta Potential. For cell viability analysis, murine fibroblastic cell lines (NIH/3T3) were plated (2.5 x 105 cells per well) 24-well plate embedded in gel (100µL). Results and discussion The analysis of cellulose suspensions through SEM, showed a significant change in the size and shape of the structures after hydrolysis, indicating the obtention of structures on a nanometric scale. For the analysis of cellulose aggregation, the zeta potential values indicated that after the addition of sodium citrate, greater dispersion was obtained between the cellulose structures, enabling resistance to the structure in a uniform way. FTIR analysis showed changes in the covalent bonds of the products. Cell viability assay showed structures containing fibroblast cells, alginate and cellulose with 1 cycle of freezing with citrate showed an intact gel structure, with cell aggregates indicating possible cell growth, while the one with only alginate showed dead cells and showed that the hydrogel did not induce cellular toxicity. These results suggest that the hydrolysis of microcrystalline cellulose can lead to obtaining cellulose nanofibers with potential for applications in tissue engineering. Conclusions Hydrogels, they have potential for applications in tissue engineering, since they have mechanical resistance and cell viability. In addition, hydrogels from exclusively vegetable sources, since these are in large quantity, low cost and environmental impact, given that the alginate comes from brown algae found in several coastal regions and the cellulose can be extracted from renewable sources or various vegetable waste from agroindustry.
dc.event.siglaCOLAOB
dc.format.extent76-76
dc.identifier.citationSILVA, GIOVANA D. da; SAKATA, SOLANGE K.; ASSIS, JOAO V.A. de; SANTOS, ESTHER C. dos; PRUDENTE, SULEYNA R.; RODRIGUES, ALEX A.; FALCAO, PATRICIA L.; VIEIRA, DANIEL P. Production of a double-network hydrogel using sodium alginate and nano-structured cellulose to 3D cell cultures. In: LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th, December 12-15, 2023, Mar del Plata, Argentina. <b>Abstract...</b> p. 76-76. Disponível em: https://repositorio.ipen.br/handle/123456789/47951.
dc.identifier.orcidhttps://orcid.org/0000-0001-6072-5853
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.urihttps://repositorio.ipen.br/handle/123456789/47951
dc.local.eventoMar del Plata, Argentina
dc.rightsopenAccess
dc.titleProduction of a double-network hydrogel using sodium alginate and nano-structured cellulose to 3D cell cultures
dc.typeResumo de eventos científicos
dspace.entity.typePublication
ipen.autorGIOVANA DIAS DA SILVA
ipen.autorSOLANGE KAZUMI SAKATA
ipen.autorJOAO VICTOR ALMEIDA DE ASSIS
ipen.autorESTHER CAROLINA DOS SANTOS
ipen.autorALEX ALVES RODRIGUES
ipen.autorPATRICIA LIMA FALCAO
ipen.autorDANIEL PEREZ VIEIRA
ipen.codigoautor15317
ipen.codigoautor7038
ipen.codigoautor15846
ipen.codigoautor15983
ipen.codigoautor15583
ipen.codigoautor16028
ipen.codigoautor3158
ipen.contributor.ipenauthorGIOVANA DIAS DA SILVA
ipen.contributor.ipenauthorSOLANGE KAZUMI SAKATA
ipen.contributor.ipenauthorJOAO VICTOR ALMEIDA DE ASSIS
ipen.contributor.ipenauthorESTHER CAROLINA DOS SANTOS
ipen.contributor.ipenauthorALEX ALVES RODRIGUES
ipen.contributor.ipenauthorPATRICIA LIMA FALCAO
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.event.datapadronizada2023
ipen.identifier.ipendoc30292
ipen.notas.internasAbstract
ipen.type.genreResumo
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sigepi.autor.atividadeSILVA, GIOVANA DIAS DA:15317:810:S
sigepi.autor.atividadeSAKATA,SOLANGE K.:7038:220:N
sigepi.autor.atividadeASSIS, JOAO V. A. DE:15846:810:N
sigepi.autor.atividadeESTHER CAROLINA DOS SANTOS:15983:-1:N
sigepi.autor.atividadeRODRIGUES, ALEX ALVES:15583:810:N
sigepi.autor.atividadePATRICIA LIMA FALCAO:16028:-1:N
sigepi.autor.atividadeVIEIRA, D.P.:3158:810:N
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