Antimicrobial photodynamic therapy on Streptococcus mutans is altered by glucose in the presence of methylene blue and red LED
Carregando...
Data
Data de publicação
Autores IPEN
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
Photodiagnosis and Photodynamic Therapy
Exportar
Resumo
Background: Dental caries are a multifactorial disease that progressively produces tooth destruction as a result of
bacterial colonization of enamel surface, especially Streptococcus mutans. The objective of this work was to
investigate the role of glucose in antimicrobial photodynamic therapy (aPDT) on S. mutans.
Methods: S. mutans ATCC 25175 were cultured on microaerophilia at 37 °C for 48 h, and we tested aPDT in the
presence of 50 mM glucose. Bacterial suspension was used to investigate aPDT with 100 μM methylene blue
(MB) under LED emitting radiation at ʎ =660 nm and parameters as following (P =473 mW; I =166.8 mW/
cm2, and doses of 5, 10 and 20 J/cm2). A seventy-two hours biofilm was grown on 96 flat buttoned well-plate
and irradiation was performed from 10 to 80 J/cm2 at similar conditions.
Results: There was no dark toxicity nor bacterial death regarding LED irradiation on suspension and on biofilm.
Nevertheless, aPDT presented expressive bacterial inactivation following 1 and 2 min of irradiation on cell
suspension. On the other hand, there was no inactivation in the presence of glucose under the same conditions.
Biofilm was completely inactivated by MB-mediated aPDT after 6 min of irradiation. However, the presence of
glucose delayed the complete inactivation of the biofilm.
Conclusion: The presence of glucose in the suspension drastically delayed the effect of aPDT on S. mutans and this
effect is more pronounced in bacterial suspension than on biofilm.
Como referenciar
LEAL, CINTIA R.L.; ALVARENGA, LETICIA H.; OLIVEIRA-SILVA, TAMIRES; KATO, ILKA T.; GODOY-MIRANDA, BIANCA; BUSSADORI, SANDRA K.; RIBEIRO, MARTHA S.; PRATES, RENATO A. Antimicrobial photodynamic therapy on Streptococcus mutans is altered by glucose in the presence of methylene blue and red LED. Photodiagnosis and Photodynamic Therapy, v. 19, p. 1-4, 2017. DOI: 10.1016/j.pdpdt.2017.04.004. Disponível em: http://repositorio.ipen.br/handle/123456789/27893. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.