Characterization of cellulose biofilm obtained from production of Kombucha
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2022
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BRAZIL MRS MEETING, 20th
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Kombucha is fermentation of green tea with sugar that results in a probiotic drink and
cellulose biofilm, in addition to alcohol, acetic acid and carbon dioxide. The mechanism is
the same as that of Jun, however the association of yeasts and bacteria is slightly different
and moved by sugar, instead of honey. It is possible that the name “Kombucha” originates
from Japanese words “kombu” which means seaweed and “cha” that stands for tea. Jun, on
the other hand, has an origin shrouded in mysteries, and may come from Tibet or even be a
modern invention, according to Sandor Katz explains in his book “art of fermentation”. In
this study, research was carried out on the sugar type influence on the growth of cellulose
matrix, or biofilm, obtained from the fermentation process of Kombucha. The material was
obtained from fermentation process of green tea with sugar. Various parameters can be
modified in the production of cellulose, such as temperature, type of tea, type of sugar,
process time, among others. In this work, for each process, first, it was necessary to obtain
the initial fermented solution: for 4 days the fermentation took place in a mixture of green
tea, water, 10% of green already fermented in a previous process and a type of sugar
(mascavo, melaço, demerara, crystal and white sugar). Fermentation took place in an
environment with a controlled temperature of 25 °C and 60% humidity. In a second step, 40
ml of fermented Kombucha (with no more sugar added) was transferred to each of the petri
dishes, containing a microscope slide, to ferment for another 4 days in order to produce a
film of cellulose that was analyzed by XRF (X-ray fluorescence), UV-Vis spectroscopy and
microscope, in addition to having its density obtained by the Archimedes method. The films
produced were pressed and dried in halogen lamp. Fifteen samples were produced. The
fermented liquid from the second phase in the petri dishes was characterized with respect
to pH, BRIX and density.
Como referenciar
SPARVOLI, MARINA; CHUBACI, JOSE F.D.; MORAIS, FERNANDO G.; JORGE, FABIO de O.; MATTA, JOSE; LANDULFO, EDUARDO. Characterization of cellulose biofilm obtained from production of Kombucha. In: BRAZIL MRS MEETING, 20th, September 25-29, 2022, Foz do Iguaçu, PR. Abstract... São Carlos, SP: Aptor Software, 2022. Disponível em: http://repositorio.ipen.br/handle/123456789/33559. Acesso em: 24 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.